| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D7 |
| Uniprot No | Q9P0N9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-293 aa |
| 活性数据 | MTEDSQRNFR SVYYEKVGFR GVEEKKSLEI LLKDDRLDTE KLCTFSQRFP LPSMYRALVW KVLLGILPPH HESHAKVMMY RKEQYLDVLH ALKVVRFVSD ATPQAEVYLR MYQLESGKLP RSPSFPLEPD DEVFLAIAKA MEEMVEDSVD CYWITRRFVN QLNTKYRDSL PQLPKAFEQY LNLEDGRLLT HLRMCSAAPK LPYDLWFKRC FAGCLPESSL QRVWDKVVSG SCKILVFVAV EILLTFKIKV MALNSAEKIT KFLENIPQDS SDAIVSKAID LWHKHCGTPV HSS |
| 分子量 | 33.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人TBC1D7蛋白的参考文献摘要(虚构示例，实际文献需通过数据库查询)：
1. **文献名称**：*TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1*
**作者**：Dibble, C.C. et al. (2013)
**摘要**：阐明了TBC1D7作为TSC1-TSC2复合物的第三亚基，调控mTORC1信号通路的作用机制，揭示其通过稳定复合物结构抑制细胞增殖。
2. **文献名称**：*Structural basis of TBC1D7 recognition by TSC complex*
**作者**：Dodonova, S.O. et al. (2017)
**摘要**：利用X射线晶体学解析TBC1D7与TSC1/TSC2的三维结构，揭示了该蛋白通过特定结构域介导复合物组装及mTOR通路调控的关键界面。
3. **文献名称**：*TBC1D7 phosphorylation regulates AMPK-mediated energy sensing*
**作者**：Pan, D. & Guan, K.L. (2016)
**摘要**：报道TBC1D7在能量应激下的磷酸化修饰，证明其通过AMPK信号通路调节细胞代谢平衡，为代谢疾病研究提供新靶点。
4. **文献名称**：*TBC1D7 mutations impair lysosomal function in cancer*
**作者**：Hahn, K. et al. (2020)
**摘要**：发现TBC1D7基因突变导致溶酶体功能障碍，促进肿瘤发生，提示其作为癌症治疗潜在靶标的可能性。
*注：以上文献及内容为模拟生成，实际研究需参考PubMed或Web of Science数据库。建议使用关键词"TBC1D7"+"mTOR"或"TBC1D7"+"TSC complex"检索最新论文。*
TBC1D7 is a conserved protein belonging to the Tre-2/Bub2/Cdc16 (TBC) domain-containing family, primarily involved in regulating cellular signaling and metabolic pathways. It acts as a key component of the mammalian target of rapamycin complex 1 (mTORC1), a central hub coordinating cell growth, proliferation, and autophagy in response to nutrient and energy availability. TBC1D7 stabilizes the mTORC1 complex by binding to Raptor, a core subunit, and helps maintain its structural integrity. Dysregulation of TBC1D7 has been implicated in metabolic disorders, tumorigenesis, and neurological conditions due to its role in mTORC1-mediated signaling.
The protein contains a C-terminal TBC domain, which typically functions as a GTPase-activating protein (GAP) for Rab-family GTPases, though TBC1D7's GAP activity remains unclear. Studies suggest it may instead act as a regulatory scaffold. Genetic mutations or altered expression of TBC1D7 are linked to conditions like cancer, tuberous sclerosis complex (TSC), and intellectual disability. Recombinant human TBC1D7 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables biochemical studies of its interactions, post-translational modifications (e.g., phosphorylation), and mechanistic contributions to mTORC1 signaling. Its recombinant form is critical for structural analysis and developing targeted therapies for mTOR-related diseases.
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