| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBX10 |
| Uniprot No | O75333 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-385 aa |
| 活性数据 | MAAFLSAGLG ILAPSETYPL PTTSSGWEPR LGSPFPSGPC TSSTGAQAVA EPTGQGPKNP RVSRVTVQLE MKPLWEEFNQ LGTEMIVTKA GRRMFPPFQV KILGMDSLAD YALLMDFIPL DDKRYRYAFH SSAWLVAGKA DPATPGRVHF HPDSPAKGAQ WMRQIVSFDK LKLTNNLLDD NGHIILNSMH RYQPRFHVVF VDPRKDSERY AQENFKSFIF TETQFTAVTA YQNHRITQLK IASNPFAKGF RESDLDSWPV APRPLLSVPA RSHSSLSPCV LKGATDREKD PNKASASTSK TPAWLHHQLL PPPEVLLAPA TYRPVTYQSL YSGAPSHLGI PRTRPAPYPL PNIRADRDQG GLPLPAGLGL LSPTVVCLGP GQDSQ |
| 分子量 | 42.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBX10蛋白的模拟参考文献(虚构内容，仅供示例参考)：
1. **文献名称**: "TBX10 regulates limb bud development through Wnt signaling modulation"
**作者**: Bamforth SD, et al.
**摘要**: 该研究通过构建重组人TBX10蛋白，探究其对小鼠胚胎肢体发育的影响，发现TBX10通过调控Wnt通路关键基因(如Wnt5a)的表达，协调肢芽细胞的增殖与分化。
2. **文献名称**: "Functional characterization of recombinant human TBX10 in cardiac progenitor cell differentiation"
**作者**: Papaioannou VE, et al.
**摘要**: 利用HEK293细胞表达系统获得高纯度重组人TBX10蛋白，并证明其通过结合心肌特异基因启动子区(如NKX2.5)，促进心脏前体细胞向心肌细胞分化。
3. **文献名称**: "TBX10 overexpression correlates with tumor suppression in gastric cancer models"
**作者**: Li H, et al.
**摘要**: 通过体外重组TBX10蛋白处理胃癌细胞系，发现其可抑制细胞迁移和侵袭能力，机制涉及下调EMT相关蛋白(如Snail和Vimentin)的表达。
4. **文献名称**: "Structural insights into the DNA-binding domain of TBX10 by recombinant protein crystallography"
**作者**: Smith J, et al.
**摘要**: 首次解析了重组人TBX10蛋白T-box结构域的晶体结构，揭示其与靶DNA结合的特异性氨基酸残基，为理解TBX家族蛋白的分子机制提供结构基础。
(注：以上文献及作者名为模拟生成，仅用于示意参考格式，实际研究需查阅真实数据库如PubMed等。)
Recombinant human TBX10 protein is a genetically engineered form of the transcription factor encoded by the *TBX10* gene, a member of the T-box family. TBX proteins are evolutionarily conserved regulators of embryonic development, particularly in organogenesis, cell fate determination, and tissue patterning. The TBX10 protein contains a conserved T-box DNA-binding domain that enables sequence-specific interactions with target genes, modulating their expression during critical developmental stages.
Studies suggest TBX10 plays roles in early cardiac development, skeletal muscle differentiation, and limb formation, though its functions are less characterized compared to other T-box members like TBX5 or TBX20. Mutations in *TBX10* have been tentatively linked to congenital abnormalities, though exact mechanisms remain under investigation. Recombinant TBX10 is typically produced in bacterial or mammalian expression systems, purified for functional studies, and used to explore its transcriptional activity, signaling pathways, or interactions with co-regulators.
This protein serves as a tool to model developmental disorders, dissect gene regulatory networks, or screen for therapeutic candidates targeting T-box-related diseases. Its recombinant form allows scalable production for biochemical assays, structural studies, or antibody development, advancing both basic research and translational applications.
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