| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBL3 |
| Uniprot No | Q12788 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-808 aa |
| 活性数据 | MAETAAGVGRFKTNYAVERKIEPFYKGGKAQLDQTGQHLFCVCGTRVNILEVASGAVLRSLEQEDQEDITAFDLSPDNEVLVTASRALLLAQWAWQEGSVTRLWKAIHTAPVATMAFDPTSTLLATGGCDGAVRVWDIVRHYGTHHFRGSPGVVHLVAFHPDPTRLLLFSSATDAAIRVWSLQDRSCLAVLTAHYSAVTSLAFSADGHTMLSSGRDKICIIWDLQSCQATRTVPVFESVEAAVLLPEEPVSQLGVKSPGLYFLTAGDQGTLRVWEAASGQCVYTQAQPPGPGQELTHCTLAHTAGVVLTATADHNLLLYEARSLRLQKQFAGYSEEVLDVRFLGPEDSHVVVASNSPCLKVFELQTSACQILHGHTDIVLALDVFRKGWLFASCAKDQSVRIWRMNKAGQVMCVAQGSGHTHSVGTVCCSRLKESFLVTGSQDCTVKLWPLPKALLSKNTAPDNGPILLQAQTTQRCHDKDINSVAIAPNDKLLATGSQDRTAKLWALPQCQLLGVFSGHRRGLWCVQFSPMDQVLATASADGTIKLWALQDFSCLKTFEGHDASVLKVAFVSRGTQLLSSGSDGLVKLWTIKNNECVRTLDAHEDKVWGLHCSRLDDHALTGASDSRVILWKDVTEAEQAEEQARQEEQVVRQQELDNLLHEKRYLRALGLAISLDRPHTVLTVIQAIRRDPEACEKLEATMLRLRRDQKEALLRFCVTWNTNSRHCHEAQAVLGVLLRREAPEELLAYEGVRAALEALLPYTERHFQRLSRTLQAAAFLDFLWHNMKLPVPAAAPTPWETHKGALP |
| 分子量 | 114.62 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于重组人TBL3蛋白的参考文献示例，涵盖其结构、功能及表达研究：
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1. **"Structural Characterization of Recombinant Human TBL3 Reveals a Novel Protein Interaction Domain"**
*Authors: Chen, L.; Wang, H.*
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了重组人TBL3蛋白，利用X射线晶体学解析其三维结构，发现一个独特的螺旋结构域，可能参与与其他调控蛋白(如HDAC3)的相互作用，为探究TBL3在转录抑制复合物中的作用机制提供结构基础。
2. **"TBL3 Mediates Lipid Metabolism by Regulating PPARγ Activity in Hepatocytes"**
*Authors: Park, S.; Jung, Y.K.*
**摘要**: 文章发现重组TBL3蛋白在肝细胞中与PPARγ直接结合，通过调控其靶基因表达影响脂质稳态。实验采用体外重组蛋白进行免疫共沉淀和荧光素酶报告基因分析，证明TBL3在代谢疾病中的潜在作用。
3. **"Optimization of Recombinant TBL3 Protein Production in Mammalian Cells for Functional Studies"**
*Authors: Müller, R.; Schmidt, B.*
**摘要**: 作者通过哺乳动物表达系统优化TBL3重组蛋白的分泌表达，验证其糖基化修饰对蛋白稳定性的影响。纯化后的蛋白用于细胞凋亡实验，显示TBL3可能通过调控Bcl-2家族蛋白抑制癌细胞死亡。
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**备注**：以上文献为示例性质，实际研究中建议通过数据库(如PubMed、Google Scholar)以“TBL3 protein recombinant”“human TBL3 function”等关键词检索最新论文。若研究较少，可扩展至TBL3同源蛋白(如TBL1XR1)或相关通路(如Wnt/β-catenin)的文献参考。
TBL3 (Transducin Beta-like Protein 3) is a member of the WD40 repeat-containing protein family, characterized by its conserved β-propeller structure that facilitates protein-protein interactions. It shares homology with β-transducin repeat-containing proteins (β-TrCPs), which are critical in ubiquitination pathways. Human TBL3. encoded by the *TBL3* gene on chromosome 16q12.2. plays roles in transcriptional regulation, cellular signaling, and protein degradation. Studies suggest its involvement in modulating Wnt/β-catenin and NF-κB pathways by interacting with key components like β-TrCP1. influencing processes such as cell proliferation, apoptosis, and inflammation. Dysregulation of TBL3 has been linked to cancers (e.g., breast, colorectal) and neurological disorders, though its exact mechanisms remain under investigation. Recombinant TBL3 protein is typically produced in *E. coli* or mammalian expression systems, often fused with tags (e.g., GST, His) for purification and functional studies. It serves as a tool to explore protein interactions, screen therapeutic agents targeting TBL3-associated pathways, or develop diagnostic biomarkers. Current research focuses on clarifying its physiological roles and potential as a drug target, particularly in diseases with disrupted ubiquitin-proteasome systems or aberrant signaling cascades.
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