| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBL2 |
| Uniprot No | Q9Y4P3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-447 aa |
| 活性数据 | MELSQMSELM GLSVLLGLLA LMATAAVARG WLRAGEERSG RPACQKANGF PPDKSSGSKK QKQYQRIRKE KPQQHNFTHR LLAAALKSHS GNISCMDFSS NGKYLATCAD DRTIRIWSTK DFLQREHRSM RANVELDHAT LVRFSPDCRA FIVWLANGDT LRVFKMTKRE DGGYTFTATP EDFPKKHKAP VIDIGIANTG KFIMTASSDT TVLIWSLKGQ VLSTINTNQM NNTHAAVSPC GRFVASCGFT PDVKVWEVCF GKKGEFQEVV RAFELKGHSA AVHSFAFSND SRRMASVSKD GTWKLWDTDV EYKKKQDPYL LKTGRFEEAA GAAPCRLALS PNAQVLALAS GSSIHLYNTR RGEKEECFER VHGECIANLS FDITGRFLAS CGDRAVRLFH NTPGHRAMVE EMQGHLKRAS NESTRQRLQQ QLTQAQETLK SLGALKK |
| 分子量 | 49.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBL2蛋白的3篇参考文献的简要信息，基于公开研究内容虚构整理(实际文献需通过数据库验证)：
---
1. **文献名称**: *Recombinant Human TBL2 Expression in E. coli: Purification and Structural Characterization*
**作者**: Smith A, et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达重组人TBL2蛋白的优化方案，通过亲和层析技术纯化蛋白，并利用圆二色光谱和X射线晶体学分析其二级结构和三维构象，证实其与WD40结构域功能的一致性。
2. **文献名称**: *TBL2 Interaction with Histone Deacetylases: A Role in Transcriptional Repression*
**作者**: Chen L, et al.
**摘要**: 作者通过体外Pull-down实验和免疫共沉淀技术，证明重组人TBL2蛋白与HDAC复合物直接互作，调控靶基因的组蛋白去乙酰化，进而抑制肿瘤细胞中的异常转录活性。
3. **文献名称**: *Functional Analysis of TBL2 Mutants in Wnt/β-catenin Signaling*
**作者**: Kimura T, et al.
**摘要**: 利用重组表达的TBL2及其突变体，研究其在Wnt通路中的作用。实验表明，TBL2通过结合β-catenin调控其稳定性，且特定位点突变导致结肠癌细胞增殖能力显著下降。
---
**注意**：以上文献信息为示例性虚构，实际引用请通过PubMed、Web of Science或Google Scholar检索真实文献，推荐关键词："recombinant TBL2 protein"或"TBL2 function"。
Recombinant human Transducin-beta-like 2 (TBL2) protein is a member of the WD40 repeat-containing protein family, characterized by its conserved beta-propeller structure that facilitates protein-protein interactions. TBL2 is encoded by the *TBL2* gene located on chromosome 7q22.1 and is ubiquitously expressed, with notable abundance in tissues like the liver, brain, and skeletal muscle. It functions as a critical regulatory component in chromatin remodeling complexes, particularly those involving histone acetyltransferases (HATs) or deacetylases (HDACs), thereby influencing transcriptional regulation and epigenetic modifications.
TBL2 interacts with transcriptional co-repressors like TBL1 and HDAC3. modulating pathways such as Wnt/β-catenin signaling and nuclear receptor-mediated gene expression. Dysregulation of TBL2 has been implicated in metabolic disorders, neurodegenerative diseases, and cancer progression. For instance, altered TBL2 expression correlates with hepatocellular carcinoma aggressiveness and lipid metabolism anomalies.
Recombinant TBL2 is produced via bacterial or mammalian expression systems, enabling biochemical studies on its structure, post-translational modifications, and interactions. Its applications include elucidating mechanisms in chromatin dynamics, drug target validation, and high-throughput screening for therapeutic agents. Recent research also explores its role in endoplasmic reticulum (ER) stress response and RNA processing. While TBL2’s precise molecular mechanisms remain under investigation, recombinant protein tools have advanced understanding of its dual roles in transcriptional regulation and cellular homeostasis, highlighting its potential as a therapeutic or diagnostic biomarker.
×
