| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBL1X |
| Uniprot No | O60907 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-577 aa |
| 活性数据 | MTELAGASSS CCHRPAGRGA MQSVLHHFQR LRGREGGSHF INTSSPRGEA KMSITSDEVN FLVYRYLQES GFSHSAFTFG IESHISQSNI NGTLVPPAAL ISILQKGLQY VEAEISINED GTVFDGRPIE SLSLIDAVMP DVVQTRQQAF REKLAQQQAS AAAAAAAATA AATAATTTSA GVSHQNPSKN REATVNGEEN RAHSVNNHAK PMEIDGEVEI PSSKATVLRG HESEVFICAW NPVSDLLASG SGDSTARIWN LNENSNGGST QLVLRHCIRE GGHDVPSNKD VTSLDWNTNG TLLATGSYDG FARIWTEDGN LASTLGQHKG PIFALKWNRK GNYILSAGVD KTTIIWDAHT GEAKQQFPFH SAPALDVDWQ NNTTFASCST DMCIHVCRLG CDRPVKTFQG HTNEVNAIKW DPSGMLLASC SDDMTLKIWS MKQEVCIHDL QAHNKEIYTI KWSPTGPATS NPNSNIMLAS ASFDSTVRLW DIERGVCTHT LTKHQEPVYS VAFSPDGKYL ASGSFDKCVH IWNTQSGNLV HSYRGTGGIF EVCWNARGDK VGASASDGSV CVLDLRK |
| 分子量 | 62.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TBL1X蛋白的模拟参考文献(基于已有研究背景，部分信息为简化示例)：
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1. **文献名称**: *Structural and functional characterization of recombinant human TBL1X in nuclear receptor co-repressor complexes*
**作者**: Smith A, et al.
**摘要**: 本研究通过表达和纯化重组人TBL1X蛋白，解析其与核受体共抑制复合物的相互作用，揭示了TBL1X通过招募组蛋白去乙酰化酶(HDACs)调控靶基因转录的分子机制。
2. **文献名称**: *TBL1X as a critical component of the Wnt/β-catenin signaling pathway*
**作者**: Lee J, et al.
**摘要**: 文章利用重组TBL1X蛋白进行体外结合实验，证明其在Wnt信号通路中通过与β-catenin直接互作，促进靶基因的激活，并揭示其在结直肠癌细胞中的致癌作用。
3. **文献名称**: *Recombinant TBL1X facilitates chromatin remodeling by interacting with the N-CoR/SMRT complex*
**作者**: Zhang Y, et al.
**摘要**: 通过体外重建染色质重塑系统，研究团队发现重组TBL1X蛋白通过桥接N-CoR/SMRT复合物与E3泛素连接酶，调控转录因子(如STAT3)的表观遗传修饰功能。
4. **文献名称**: *Biochemical analysis of TBL1X mutations linked to human hearing loss*
**作者**: Wang R, et al.
**摘要**: 该研究制备了多种重组TBL1X突变蛋白，发现其与耳蜗发育相关基因的异常互作导致蛋白质稳定性下降，从而阐明了TBL1X缺陷引发遗传性耳聋的分子基础。
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**说明**：以上内容根据TBL1X已知功能(如核受体调控、Wnt信号、表观遗传修饰)及重组蛋白研究场景模拟，实际文献需通过PubMed/Web of Science等平台检索。
Transducin beta-like 1 X-linked (TBL1X) is a member of the WD40 repeat protein family and a critical component of nuclear receptor corepressor (NCoR) and silencing mediator for retinoid and thyroid hormone receptors (SMRT) complexes. Functioning as a scaffold protein, it mediates transcriptional repression by recruiting histone deacetylases (HDACs) to target genes, thereby modulating chromatin structure and gene expression. TBL1X also plays a role in activating transcription by facilitating the exchange of corepressors for coactivators in response to ligand binding, particularly in nuclear receptor signaling pathways. Additionally, it interacts with the Wnt/β-catenin pathway, influencing cell proliferation, differentiation, and apoptosis.
Mutations in the TBL1X gene are linked to X-linked syndromes, including hearing loss and intellectual disability. Dysregulation of TBL1X is implicated in cancers (e.g., breast, leukemia) and neuropsychiatric disorders, often due to aberrant protein interactions or impaired HDAC recruitment. Recombinant TBL1X protein, typically expressed in *E. coli* or mammalian systems with affinity tags (e.g., His-tag), retains native structural and functional properties, enabling biochemical studies, interaction assays, and mechanistic investigations. Its phosphorylation and ubiquitination sites are key regulatory features. Current research focuses on therapeutic targeting of TBL1X in cancer and genetic disorders, leveraging recombinant forms for drug screening and structural analysis to design pathway-specific inhibitors or stabilizers.
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