| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBX19 |
| Uniprot No | O60806 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-448 aa |
| 活性数据 | MAMSELGTRK PSDGTVSHLL NVVESELQAG REKGDPTEKQ LQIILEDAPL WQRFKEVTNE MIVTKNGRRM FPVLKISVTG LDPNAMYSLL LDFVPTDSHR WKYVNGEWVP AGKPEVSSHS CVYIHPDSPN FGAHWMKAPI SFSKVKLTNK LNGGGQIMLN SLHKYEPQVH IVRVGSAHRM VTNCSFPETQ FIAVTAYQNE EITALKIKYN PFAKAFLDAK ERNHLRDVPE AISESQHVTY SHLGGWIFSN PDGVCTAGNS NYQYAAPLPL PAPHTHHGCE HYSGLRGHRQ APYPSAYMHR NHSPSVNLIE SSSNNLQVFS GPDSWTSLSS TPHASILSVP HTNGPINPGP SPYPCLWTIS NGAGGPSGPG PEVHASTPGA FLLGNPAVTS PPSVLSTQAP TSAGVEVLGE PSLTSIAVST WTAVASHPFA GWGGPGAGGH HSPSSLDG |
| 分子量 | 48.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**：*Functional analysis of TBX19 mutations in congenital adrenal hyperplasia*
**作者**：Pinto G et al.
**摘要内容**：研究揭示了重组人TBX19蛋白突变如何导致先天性肾上腺发育不良，通过体外实验表明突变破坏了其与DNA结合能力，影响促肾上腺皮质激素(ACTH)调控基因的表达。
2. **文献名称**：*Role of TBX19 in pituitary development and regulation of pro-opiomelanocortin transcription*
**作者**：Lamolet B et al.
**摘要内容**：阐明了重组TBX19蛋白在垂体前叶细胞中的关键作用，证明其通过结合特定DNA序列激活POMC基因转录，对肾上腺应激反应通路至关重要。
3. **文献名称**：*Generation and characterization of recombinant human TBX19 for structural studies*
**作者**：Zhang Y et al.
**摘要内容**：报道了重组人TBX19蛋白的高效原核表达及纯化方法，并通过X射线晶体学解析其T-box结构域的三维结构，为突变相关疾病机制提供分子基础。
4. **文献名称**：*TBX19 regulates endocrine cell differentiation in human pluripotent stem cells*
**作者**：Huang S et al.
**摘要内容**：利用重组TBX19蛋白诱导人多能干细胞定向分化为促肾上腺皮质激素细胞，证实其在体外模型中促进肾上腺相关激素表达和细胞功能成熟的作用。
**Background of Recombinant Human TBX19 Protein**
TBX19. also known as TPIT, is a member of the T-box transcription factor family characterized by a conserved DNA-binding domain called the T-box. This protein plays a critical role in embryonic development, particularly in the differentiation and function of pituitary corticotroph and melanotroph cells. TBX19 regulates the expression of proopiomelanocortin (POMC), a precursor peptide essential for synthesizing adrenocorticotropic hormone (ACTH) and other bioactive molecules involved in stress response and pigmentation.
Mutations in the TBX19 gene are linked to congenital isolated ACTH deficiency, a rare disorder marked by adrenal insufficiency due to impaired ACTH production. Recombinant human TBX19 protein, produced via genetic engineering in systems like *E. coli* or mammalian cells, retains the functional T-box domain, enabling studies on its DNA-binding activity and regulatory mechanisms. This recombinant form is vital for exploring TBX19's role in cell differentiation, hormone regulation, and disease pathology. It also serves as a tool for drug screening, disease modeling, and developing therapeutic strategies targeting pituitary disorders. High-purity recombinant TBX19. often fused with tags like His or GST, ensures reliability in biochemical assays, structural studies, and functional genomics research.
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