| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBX20 |
| Uniprot No | Q9UMR3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-447 aa |
| 活性数据 | MEFTASPKPQ LSSRANAFSI AALMSSGGSK EKEATENTIK PLEQFVEKSS CAQPLGELTS LDAHGEFGGG SGSSPSSSSL CTEPLIPTTP IIPSEEMAKI ACSLETKELW DKFHELGTEM IITKSGRRMF PTIRVSFSGV DPEAKYIVLM DIVPVDNKRY RYAYHRSSWL VAGKADPPLP ARLYVHPDSP FTGEQLLKQM VSFEKVKLTN NELDQHGHII LNSMHKYQPR VHIIKKKDHT ASLLNLKSEE FRTFIFPETV FTAVTAYQNQ LITKLKIDSN PFAKGFRDSS RLTDIERESV ESLIQKHSYA RSPIRTYGGE EDVLGDESQT TPNRGSAFTT SDNLSLSSWV SSSSSFPGFQ HPQSLTALGT STASIATPIP HPIQGSLPPY SRLGMPLTPS AIASSMQGSG PTFPSFHMPR YHHYFQQGPY AAIQGLRHSS AVMTPFV |
| 分子量 | 49.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TBX20蛋白的参考文献摘要示例(注意：部分信息为模拟内容，实际引用请核实原始文献)：
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1. **文献名称**: "TBX20 regulates angiogenesis through interaction with the VEGF signaling pathway"
**作者**: Wang, Y., et al.
**摘要**: 该研究发现TBX20蛋白通过直接结合血管内皮生长因子受体2(VEGFR2)的启动子区域调控其表达，促进血管生成。重组人TBX20蛋白在体外实验中显著增强内皮细胞迁移能力，提示其在缺血性疾病治疗中的潜在应用。
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2. **文献名称**: "Structural insights into TBX20-DNA interaction and its role in congenital heart defects"
**作者**: Smith, J.R., et al.
**摘要**: 通过X射线晶体学解析了TBX20蛋白的T-box结构域与DNA结合的复合物结构，揭示其特异性识别DNA基序的分子机制。实验发现特定错义突变(如Q195R)会破坏TBX20与DNA的结合能力，与人类先天性心脏病发病密切相关。
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3. **文献名称**: "Recombinant TBX20 protein promotes cardiomyocyte differentiation from human iPSCs"
**作者**: Tanaka, M., et al.
**摘要**: 研究证实重组TBX20蛋白可激活心脏发育关键基因NKX2-5和GATA4的表达。通过蛋白质微阵列分析发现，TBX20与Wnt/β-catenin通路存在交叉调控，为心脏再生医学提供了新的分子调控靶点。
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(注：以上为学术文献格式参考，实际文献请通过PubMed或Web of Science查询，关键词：TBX20 recombinant protein, transcriptional regulation, heart development)
T-box transcription factor 20 (TBX20) is a member of the evolutionarily conserved T-box gene family, which plays pivotal roles in embryonic development, particularly in heart formation and morphogenesis. The TBX20 protein contains a characteristic DNA-binding domain called the T-box, enabling it to regulate the expression of target genes involved in cellular differentiation and tissue patterning. In humans, TBX20 is essential during cardiovascular development, influencing heart chamber specification, atrioventricular septation, and valve formation. It also contributes to maintaining adult cardiac function by modulating cardiomyocyte proliferation, apoptosis, and electrical conduction pathways.
Mutations in the TBX20 gene are associated with congenital heart defects (CHDs), such as atrial/ventricular septal defects, dilated cardiomyopathy, and arrhythmias, highlighting its clinical relevance. Recombinant human TBX20 protein, typically produced via bacterial or mammalian expression systems, serves as a critical tool for studying molecular mechanisms of heart development and disease. Researchers utilize it to investigate DNA-protein interactions, transcriptional regulation networks, and its synergy with other cardiac transcription factors like NKX2-5 and GATA4. Additionally, it aids in drug discovery for cardiac regeneration therapies and diagnostic assays for genetic screening. The protein’s functional characterization continues to advance our understanding of cardiovascular biology and potential therapeutic strategies.
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