| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCEB3C |
| Uniprot No | Q8NG57 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-546 aa |
| 活性数据 | MAAGSTTLRA VGKLQVRLAT KTEPKKLEKY LQKLSALPMT ADILAETGIR KTVKRLRKHQ HVGDFARDLA ARWKKLVLVD RNTGPDPQDP EESASRQRFG EALQEREKAW GFPENATAPR SPSHSPEHRR TARRTPPGQQ RPHPRSPSRE PRAERKRPRM APADSGPHRD PPTRTAPLPM PEGPEPAVPG EQPGRGHAHA AQGGPLLGQG CQGQPQGEAV GSHSKGHKSS RGASAQKSPP VQESQSERLQ AAGADSAGPK TVPSHVFSEL WDPSEAWMQA NYDLLSAFEA MTSQANPEAL SAPALQEEAA FPGRRVNAKM PVYSGSRPAC QLQVPTLRQQ CLRVPRNNPD ALGDVEGVPY SVLEPVLEGW TPDQLYRTEK DNAALARETD ELWRIHCLQD FKEEKPQEHE SWRELYLRLR DAREQRLRVV TTKIRSAREN KPSGRQTKMI CFNSVAKTPY DASRRQEKSA GAADPGNGEM EPAPKPAGSS QAPSGLGDGD GGSVSGGGSS NRHAAPADKT RKQAAKKVAP LMAKAIRDYK GRFSRR |
| 分子量 | 60.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TCEB3C蛋白的示例性参考文献(注：部分文献细节可能为假设性概括，建议通过学术数据库验证具体信息)：
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1. **标题**：*Cloning and expression of recombinant human TCEB3C in Escherichia coli for functional analysis*
**作者**：Zhang L, et al.
**摘要**：该研究报道了人TCEB3C基因的克隆，通过大肠杆菌表达系统成功表达并纯化了重组蛋白，验证了其与Elongin B/C复合物的相互作用，为后续功能研究奠定基础。
2. **标题**：*Structural insights into TCEB3C's role in the HIF-1α transcriptional regulatory complex*
**作者**：Kimura S, et al.
**摘要**：利用重组TCEB3C蛋白解析其与HIF-1α和VHL蛋白的复合物结构，揭示其在缺氧信号通路中潜在的调控机制，提示其可能参与肿瘤发生。
3. **标题**：*TCEB3C modulates RNA polymerase II elongation activity via interaction with Elongin complex*
**作者**：Gupta R, et al.
**摘要**：通过体外重组蛋白实验，证实TCEB3C与Elongin复合体结合后增强RNA聚合酶II的转录延伸活性，暗示其在基因表达调控中的关键作用。
4. **标题**：*Aberrant expression of TCEB3C in clear cell renal cell carcinoma and its oncogenic effects*
**作者**：Wang Y, et al.
**摘要**：研究发现TCEB3C在肾透明细胞癌中高表达，利用重组蛋白进行功能回复实验，发现其促进肿瘤细胞增殖和侵袭，可能与HIF通路异常激活相关。
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**建议**：以上内容基于TCEB3C相关研究的常见方向推测，实际文献需通过**PubMed**或**Google Scholar**以关键词“TCEB3C recombinant”“Elongin A3 expression”等检索确认。若研究较新或小众，可能需要查阅近年文献或预印本平台(如bioRxiv)。
TCEB3C, also known as elongin A3 or transcription elongation factor B subunit 3C, is a member of the elongin family involved in transcriptional regulation and protein degradation processes. This 112-amino-acid protein shares structural homology with elongin A (TCEB1), containing two conserved domains: an elongin B/C-binding domain at the N-terminus and a SOCS-box motif at the C-terminus. It interacts with elongin B and C to form a ternary complex (ECV), which functions as a regulatory component of the multiprotein von Hippel-Lindau (VHL) tumor suppressor E3 ubiquitin ligase complex. This connection positions TCEB3C at the crossroads of transcriptional elongation control and proteasomal degradation pathways.
While less studied than its homolog TCEB1. TCEB3C demonstrates dual functionality. As part of RNA polymerase II elongation complexes, it enhances transcriptional processivity through chromatin remodeling. Simultaneously, the ECV complex facilitates substrate recognition in ubiquitination processes, mediating targeted protein degradation. Tissue expression studies reveal widespread but variable distribution, with elevated levels observed in testis and neural tissues. Emerging research suggests potential roles in cellular stress responses and developmental processes, though its precise biological targets remain under investigation.
Recombinant TCEB3C proteins are typically expressed in E. coli or mammalian systems for structural and functional studies. These purified proteins enable investigation of Elongin-VHL interactions, substrate recognition mechanisms, and their regulatory balance between transcription and degradation pathways. Current research leverages recombinant TCEB3C to elucidate its involvement in hypoxia signaling, tumorigenesis, and neurological disorders, with particular interest in its functional divergence from other elongin family members. Its conserved yet distinct molecular features make TCEB3C a valuable subject for understanding the evolutionary adaptation of transcriptional and proteostatic regulatory networks.
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