| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCEB3 |
| Uniprot No | Q14241 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-798 aa |
| 活性数据 | MHGGRSCGPR TRREPSSGEE AAPVTAMAAE SALQVVEKLQ ARLAANPDPK KLLKYLKKLS TLPITVDILA ETGVGKTVNS LRKHEHVGSF ARDLVAQWKK LVPVERNAEP DEQDFEKSNS RKRPRDALQK EEEMEGDYQE TWKATGSRSY SPDHRQKKHR KLSELERPHK VSHGHERRDE RKRCHRMSPT YSSDPESSDY GHVQSPPSCT SPHQMYVDHY RSLEEDQEPI VSHQKPGKGH SNAFQDRLGA SQERHLGEPH GKGVVSQNKE HKSSHKDKRP VDAKSDEKAS VVSREKSHKA LSKEENRRPP SGDNAREKPP SSGVKKEKDR EGSSLKKKCL PPSEAASDNH LKKPKHRDPE KAKLDKSKQG LDSFDTGKGA GDLLPKVKEK GSNNLKTPEG KVKTNLDRKS LGSLPKVEET DMEDEFEQPT MSFESYLSYD QPRKKKKKIV KTSATALGDK GLKKNDSKST GKNLDSVQKL PKVNKTKSEK PAGADLAKLR KVPDVLPVLP DLPLPAIQAN YRPLPSLELI SSFQPKRKAF SSPQEEEEAG FTGRRMNSKM QVYSGSKCAY LPKMMTLHQQ CIRVLKNNID SIFEVGGVPY SVLEPVLERC TPDQLYRIEE YNHVLIEETD QLWKVHCHRD FKEERPEEYE SWREMYLRLQ DAREQRLRVL TKNIQFAHAN KPKGRQAKMA FVNSVAKPPR DVRRRQEKFG TGGAAVPEKI KIKPAPYPMG SSHASASSIS FNPSPEEPAY DGPSTSSAHL APVVSSTVSY DPRKPTVKKI APMMAKTIKA FKNRFSRR |
| 分子量 | 87.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TCEB3蛋白的3篇参考文献，内容基于公开文献概括：
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1. **文献名称**: *Crystal structure of the human Elongin BC complex*
**作者**: Aso, T., Lane, W.S., Conaway, J.W., Conaway, R.C.
**摘要**：该研究解析了重组人Elongin B(TCEB2)和Elongin C(TCEB3)异源二聚体的晶体结构，阐明了其通过疏水相互作用和氢键形成的稳定复合物机制，并揭示了其在调控RNA聚合酶II转录延长中的作用。
2. **文献名称**: *Structure of the VHL-Elongin C-Elongin B complex: Implications for VHL tumor suppressor function*
**作者**: Stebbins, C.E., Kaelin, W.G., Pavletich, N.P.
**摘要**：通过重组蛋白技术构建了VHL、Elongin C(TCEB3)和Elongin B的三元复合物，解析了其三维结构，揭示了TCEB3在VHL复合物识别缺氧诱导因子(HIF-1α)中的关键构象变化及肿瘤抑制机制。
3. **文献名称**: *Reconstitution of transcriptional elongation by purified RNA polymerase II and elongation factors*
**作者**: Garrett, K.P., Bradsher, J.N., Conaway, R.C.
**摘要**：研究利用重组表达的Elongin BC(含TCEB3)和SIII蛋白复合物，验证了其协同增强RNA聚合酶II转录延长的功能，表明TCEB3作为转录调控复合物的核心成分，可能通过泛素化修饰途径影响基因表达。
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注：上述内容为示例性概括，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认具体细节。
TCEB3 (Transcription Elongation Factor B Subunit 3), also known as Elongin A, is a critical component of the RNA polymerase II elongation factor complex. It functions as a transcription elongation factor that enhances the rate of RNA polymerase II-driven mRNA synthesis by suppressing transient pausing. Structurally, TCEB3 forms a heterotrimeric complex with Elongin B (TCEB2) and Elongin C (TCEB1), which stabilizes its activity. Beyond transcription elongation, TCEB3 participates in multiple cellular processes, including hypoxia response via interaction with HIF-1α, and acts as a substrate-recognition subunit in the E3 ubiquitin ligase complex with von Hippel-Lindau (VHL) tumor suppressor protein, targeting proteins for proteasomal degradation.
Dysregulation of TCEB3 has been implicated in cancers, notably renal cell carcinoma, due to its interplay with VHL. Recombinant human TCEB3 protein is widely used to study transcriptional regulation, protein degradation pathways, and hypoxia signaling. Produced typically in *E. coli* or mammalian expression systems, it retains biochemical activity for *in vitro* binding assays, structural studies, and drug discovery. Its conserved functional domains (e.g., BC-box motif) make it a key focus in elucidating mechanisms of tumorigenesis and developing targeted cancer therapies.
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