| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCP11L1 |
| Uniprot No | Q9NUJ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-509 aa |
| 活性数据 | MSENLDKSNV NEAGKSKSND SEEGLEDAVE GADEALQKAI KSDSSSPQRV QRPHSSPPRF VTVEELLETA RGVTNMALAH EIVVNGDFQI KPVELPENSL KKRVKEIVHK AFWDCLSVQL SEDPPAYDHA IKLVGEIKET LLSFLLPGHT RLRNQITEVL DLDLIKQEAE NGALDISKLA EFIIGMMGTL CAPARDEEVK KLKDIKEIVP LFREIFSVLD LMKVDMANFA ISSIRPHLMQ QSVEYERKKF QEILERQPNS LDFVTQWLEE ASEDLMTQKY KHALPVGGMA AGSGDMPRLS PVAVQNYAYL KLLKWDHLQR PFPETVLMDQ SRFHELQLQL EQLTILGAVL LVTFSMAAPG ISSQADFAEK LKMIVKILLT DMHLPSFHLK DVLTTIGEKV CLEVSSCLSL CGSSPFTTDK ETVLKGQIQA VASPDDPIRR IMESRILTFL ETYLASGHQK PLPTVPGGLS PVQRELEEVA IKFARLVNYN KMVFCPYYDA ILSKILVRS |
| 分子量 | 57.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TCP11L1蛋白的虚构参考示例，涵盖不同研究方向：
1. **《Cloning and functional characterization of human TCP11L1 in reproductive tissues》**
- **作者**: Zhang Y. et al.
- **摘要**: 本研究成功克隆了TCP11L1基因，并在HEK293细胞中表达了重组蛋白。实验表明，TCP11L1在睾丸组织中高表达，可能与精子发生过程中的蛋白质相互作用有关。
2. **《Structural insights into TCP11L1: X-ray crystallography and biochemical analysis》**
- **作者**: Lee S. et al.
- **摘要**: 通过X射线晶体学解析了重组TCP11L1蛋白的三维结构，揭示了其保守的TPR(tetratricopeptide repeat)结构域，并探讨其可能参与的信号转导通路。
3. **《TCP11L1 overexpression promotes cancer cell migration via EGFR signaling》**
- **作者**: Chen X. et al.
- **摘要**: 利用重组TCP11L1蛋白进行体外功能实验，发现其在肺癌细胞中过表达可激活EGFR通路，促进细胞迁移和侵袭，提示其作为潜在肿瘤标志物的可能性。
4. **《Recombinant TCP11L1 purification and interaction screening by yeast two-hybrid》**
- **作者**: Gupta R. et al.
- **摘要**: 开发了一种高效的大肠杆菌表达系统用于重组TCP11L1蛋白的纯化，并通过酵母双杂交技术鉴定了多个互作蛋白，包括参与染色体稳定的候选分子。
(注：上述文献及作者为虚构示例，实际研究中需查询真实数据库并验证。)
**Background of Recombinant Human TCP11L1 Protein**
The T-complex 11-like 1 (TCP11L1) protein, a member of the TCP11/t-complex protein family, is a conserved eukaryotic protein implicated in diverse cellular processes. Structurally, it shares homology with chaperonin-containing TCP1 (CCT) complex components, characterized by conserved domains involved in protein folding and interactions. TCP11L1 is predominantly expressed in testicular tissues, suggesting a role in spermatogenesis or fertilization, though its exact physiological function remains under investigation.
Recombinant human TCP11L1 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*), enabling high-purity protein for functional studies. Studies indicate its involvement in cytoskeletal organization, cell cycle regulation, and possibly neurodegenerative pathways. It has also been linked to cancer progression, with aberrant expression observed in certain malignancies. Research utilizes recombinant TCP11L1 to explore its interactome, substrate specificity, and mechanism in disease models.
Despite progress, the protein's full biological significance and regulatory networks are unclear. Ongoing research aims to clarify its role in fertility, disease, and potential therapeutic targeting. Recombinant TCP11L1 serves as a critical tool for structural analysis, antibody development, and drug screening platforms.
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