| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TEKT1 |
| Uniprot No | Q969V4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418 aa |
| 活性数据 | MAKLLQPPPK FLPSEWHIAN KNQYHRADAQ RSRSERLVAE SQRLVDEIEK TTRKSQSDVN KKLEQRLEEV QFWKKELDDK LEQLVNVTDD LLIYKIRLEK ALETLKEPLH ITETCLAYRE KRIGIDLVHD TVEHELIKEA EIIQGIMALL TRTLEEASEQ IRMNRSAKYN LEKDLKDKFV ALTIDDICFS LNNNSPNIRY SENAVRIEPN SVSLEDWLDF SSTNVEKADK QRNNSLMLKA LVDRILSQTA NDLRKQCDVV DTAFKNGLKD TKDARDKLAD HLAKVMEEIA SQEKNITALE KAILDQEGPA KVAHTRLETR THRPNVELCR DVAQYRLMKE VQEITHNVAR LKETLAQAQA ELKGLHRRQL ALQEEIQVKE NTIYIDEVLC MQMRKSIPLR DGEDHGVWAG GLRPDAVC |
| 分子量 | 48.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性示例(非真实文献)，供参考：
1. **文献名称**: "重组人TEKT1蛋白的表达及其在精子鞭毛结构中的功能分析"
**作者**: Zhang Y, et al.
**摘要**: 研究通过大肠杆菌系统成功表达重组TEKT1蛋白，并证实其在体外能促进微管聚合，暗示其在精子运动中的作用。
2. **文献名称**: "TEKT1基因敲除小鼠模型揭示其不育表型机制"
**作者**: Tanaka H, et al.
**摘要**: 利用CRISPR技术构建TEKT1敲除小鼠，发现雄性不育，精细胞微管结构紊乱，表明TEKT1对精子形成至关重要。
3. **文献名称**: "TEKT1与卵巢癌迁移能力的关联研究"
**作者**: Li X, et al.
**摘要**: 首次发现TEKT1在卵巢癌细胞中高表达，并通过重组蛋白实验证明其通过调控细胞骨架加速癌细胞转移。
4. **文献名称**: "重组TEKT1晶体结构解析揭示其微管结合域"
**作者**: Wang R, et al.
**摘要**: 利用X射线衍射解析TEKT1三维结构，发现其C端结构域为微管相互作用关键区域，为靶向药物设计提供依据。
**注意**：以上为模拟数据，实际文献请通过 **PubMed/Google Scholar** 查询关键词 "TEKT1 recombinant protein" 或联系相关领域数据库获取。
Tektin-1 (TEKT1) is a member of the tektin protein family, which are evolutionarily conserved filamentous proteins critical for the structural integrity and function of microtubule-based organelles. Primarily localized in cilia, flagella, and centrosomes, TEKT1 contributes to the assembly and stability of axonemal microtubules in motile structures, such as sperm flagella and respiratory cilia. It is encoded by the *TEKT1* gene and shares characteristic coiled-coil domains with other tektins, enabling protein-protein interactions essential for forming structural scaffolds.
Studies highlight TEKT1's role in male fertility, as it is highly expressed in testicular germ cells and vital for sperm motility. Mutations or dysregulation of TEKT1 have been linked to asthenozoospermia (reduced sperm movement) and ciliopathies, disorders arising from defective cilia. Additionally, TEKT1 may participate in cell cycle regulation and mitotic spindle organization, suggesting broader cellular roles beyond motility.
Recombinant human TEKT1 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cell cultures) for functional studies, antibody development, and therapeutic exploration. Its recombinant form aids in elucidating molecular mechanisms in infertility and ciliary diseases, offering potential diagnostic or therapeutic targets. Despite progress, TEKT1's precise interactions and regulatory pathways remain under investigation, underscoring its complexity in cellular dynamics and human health.
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