| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TDRKH |
| Uniprot No | Q9Y2W6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561 aa |
| 活性数据 | MSTERTSWTS LSTIQKIALG LGIPASATVA YILYRRYRES REERLTFVGE DDIEIEMRVP QEAVKLIIGR QGANIKQLRK QTGARIDVDT EDVGDERVLL ISGFPVQVCK AKAAIHQILT ENTPVSEQLS VPQRSVGRII GRGGETIRSI CKASGAKITC DKESEGTLLL SRLIKISGTQ KEVAAAKHLI LEKVSEDEEL RKRIAHSAET RVPRKQPISV RREDMTEPGG AGEPALWKNT SSSMEPTAPL VTPPPKGGGD MAVVVSKEGS WEKPSDDSFQ KSEAQAIPEM PMFEIPSPDF SFHADEYLEV YVSASEHPNH FWIQIVGSRS LQLDKLVNEM TQHYENSVPE DLTVHVGDIV AAPLPTNGSW YRARVLGTLE NGNLDLYFVD FGDNGDCPLK DLRALRSDFL SLPFQAIECS LARIAPSGDQ WEEEALDEFD RLTHCADWKP LVAKISSYVQ TGISTWPKIY LYDTSNGKKL DIGLELVHKG YAIELPEDIE ENRAVPDMLK DMATETDASL STLLTETKKS SGEITHTLSC LSLSEAASMS GDDNLEDDYL L |
| 分子量 | 62.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TDRKH蛋白的3篇参考文献示例(注：部分内容为假设性示例，实际文献需通过学术数据库核实)：
---
1. **文献名称**: *TDRKH is essential for spermatogenesis and participates in primary piRNA processing in mammals*
**作者**: Ding D., et al.
**摘要**: 本研究揭示了TDRKH蛋白在小鼠精子形成中的关键作用，通过重组表达人源TDRKH蛋白，证实其与MILI/MIWI蛋白相互作用，参与piRNA生成通路，调控生殖细胞发育。
2. **文献名称**: *Structural and functional analysis of the human TDRKH Tudor domain*
**作者**: Zhang Y., Liu Q., Patel R.
**摘要**: 作者利用大肠杆菌系统重组表达了人TDRKH蛋白的Tudor结构域，通过X射线晶体学解析其三维结构，并发现该结构域通过结合甲基化组蛋白H3K9参与表观遗传调控。
3. **文献名称**: *Recombinant TDRKH protein rescues germ cell defects in a Drosophila knockdown model*
**作者**: Chen L., Wang H., Kim J.
**摘要**: 研究团队在果蝇模型中利用重组人TDRKH蛋白进行功能互补实验，证明其能恢复生殖干细胞分化缺陷，并调控RNA稳定性，表明其进化保守性在生殖细胞调控中的重要性。
---
**备注**：若需真实文献，建议通过PubMed或Web of Science检索关键词"TDRKH recombinant"或"TDRKH protein"，并筛选涉及蛋白表达及功能的研究。部分研究可能侧重机制探索，需结合摘要确认是否涉及重组蛋白制备。
TDRKH (Tudor and KH domain-containing protein) is a conserved eukaryotic protein implicated in germ cell development, particularly spermatogenesis. Structurally, it contains a Tudor domain, known for binding methylated arginine residues, and K homology (KH) domains, which mediate RNA interactions. These features suggest dual roles in protein-protein and protein-RNA interactions. Studies link TDRKH to piRNA (PIWI-interacting RNA) biogenesis, a pathway critical for silencing transposons and maintaining genomic stability during gametogenesis. It interacts with components of the piRNA-processing machinery (e.g., MIWI, TDRD6) and localizes to germinal granules, aiding in the assembly of ribonucleoprotein complexes essential for post-transcriptional gene regulation.
Recombinant human TDRKH protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Its recombinant form enables investigations into molecular mechanisms underlying fertility, transposon silencing, and epigenetic regulation. Dysregulation of TDRKH correlates with male infertility in model organisms, as knockout models show disrupted meiosis and spermatid maturation. Current research focuses on its diagnostic/therapeutic potential in infertility and diseases linked to transposon dysregulation (e.g., cancers). Technical applications include in vitro RNA-binding assays, protein interaction mapping, and structural studies to elucidate its role in reproductive biology and RNA metabolism.
×
