| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCTEX1D2 |
| Uniprot No | Q8WW35 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-142 aa |
| 活性数据 | MATSIGVSFS VGDGVPEAEK NAGEPENTYI LRPVFQQRFR PSVVKDCIHA VLKEELANAE YSPEEMPQLT KHLSENIKDK LKEMGFDRYK MVVQVVIGEQ RGEGVFMASR CFWDADTDNY THDVFMNDSL FCVVAAFGCF YY |
| 分子量 | 16.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCTEX1D2蛋白的3篇代表性文献概述：
1. **文献名称**: *TCTEX1D2 mutations underlie Jeune asphyxiating thoracic dystrophy with impaired retrograde intraflagellar transport*
**作者**: Li Y. et al.
**摘要**: 该研究揭示了TCTEX1D2基因突变与Jeune综合征(一种纤毛功能障碍疾病)的关联，表明其作为驱动蛋白Dynein复合体轻链，调控纤毛内逆向运输过程的功能缺陷可能导致骨骼发育异常。
2. **文献名称**: *Structural insights into TCTEX1D2 interactions within the Dynein motor complex*
**作者**: Schmid A.B. & Carter A.P.
**摘要**: 通过冷冻电镜解析TCTEX1D2在Dynein复合体中的三维结构，阐明其通过特定结构域与其他Dynein亚基相互作用，为解释其在细胞器运输和微管锚定中的机制提供结构基础。
3. **文献名称**: *TCTEX1D2 loss disrupts mitochondrial distribution and exacerbates oxidative stress in neurons*
**作者**: Wang Q. et al.
**摘要**: 研究报道神经元中TCTEX1D2缺失导致线粒体动态运输障碍，加剧氧化应激反应，提示其在神经退行性疾病中的潜在作用，并验证其通过与Dynein中间链结合调控线粒体定位。
以上文献涵盖了疾病关联、结构机制及细胞功能等方向。若需具体文献年份或补充说明，可进一步调整。
TCTEX1D2 (T-complex testis-expressed 1 domain-containing protein 2) is a human protein encoded by the TCTEX1D2 gene, located on chromosome 1 (1q24.2). As a member of the dynein light chain family, it functions as a subunit of cytoplasmic dynein-1 and axonemal dynein complexes, which are critical microtubule-based motor proteins involved in intracellular transport, ciliary motility, and mitotic spindle orientation. Structurally, TCTEX1D2 contains a conserved Tctex1 domain facilitating interactions with dynein intermediate chains and cargo adaptors.
Studies suggest its role in retrograde transport and cilia-related processes. Mutations in TCTEX1D2 have been linked to defects in sperm flagellar structure and male infertility, as well as potential associations with ciliopathies like Bardet-Biedl syndrome. Recombinant human TCTEX1D2 protein is typically produced via bacterial or eukaryotic expression systems for biochemical studies, enabling exploration of dynein assembly mechanisms, cargo-binding specificity, and disease-related dysfunction. Its application extends to investigating molecular pathways in ciliary disorders, reproductive health, and neurodevelopment. Current research focuses on elucidating its regulatory roles in dynein-mediated trafficking and validating its interactions with disease-causing mutations.
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