| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCP11 |
| Uniprot No | Q8WWU5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-441 aa |
| 活性数据 | MAPKGILGSFPTAMNLSLEGKVKETVHNAFWDHLKEQLSATPPDFSCALELLKEIKEILLSLLLPRQNRLRIEIEEALDMDLLKQEAEHGALKVLYLSKYVLNMMALLCAPVRDEAVQKLENITDPVWLLRGIFQVLGRMKMDMVNYTIQSLQPHLQEHSIQYERAKFQELLNKQPSLLNHTTKWLTQAAGDLTMSPPTCPDTSDSSSVAGPSPNEAANNPEPLSPTMVLCQGFLNLLLWDLENEEFPETLLMDRTRLQELKSQLHQLTVMASVLLVASSFSGSVLFGSPQFVDKLKRITKSLLEDFHSRPEEAILTVSEQVSQEIHQSLKNMGLVALSSDNTASLMGQLQNIAKKENCVCSVIDQRIHLFLKCCLVLGVQRSLLDLPGGLTLIEAELAELGQKFVNLTHHNQQVFGPYYTEILKTLISPAQALETKVESV |
| 分子量 | 75.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TCP11蛋白的3篇参考文献示例，按文献名称、作者、摘要内容概括列出：
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1. **文献名称**: *"Cloning and Characterization of Human TCP11. a Gene Involved in Spermatogenesis"*
**作者**: Li Y, et al.
**摘要**: 该研究克隆了人TCP11基因，并发现其特异性高表达于睾丸组织中。重组TCP11蛋白在大肠杆菌中表达并纯化，功能分析表明其可能参与精子形成过程中的同源重组修复机制，与男性不育存在潜在关联。
2. **文献名称**: *"Structural Insights into TCP11 Protein Function in Murine Gametogenesis"*
**作者**: Zhang X, Wang L.
**摘要**: 通过重组表达小鼠TCP11蛋白并进行结晶分析，揭示了其N端结构域的α螺旋特征。实验表明，TCP11与减数分裂过程中染色体联会的调控相关，提示其在生殖细胞发育中的保守作用。
3. **文献名称**: *"TCP11 as a Novel Cancer-Testis Antigen: Expression and Immunogenicity Analysis"*
**作者**: Chen H, et al.
**摘要**: 研究评估了TCP11在多种肿瘤中的表达模式，发现其作为癌睾抗原的特性。利用昆虫细胞系统重组表达人TCP11蛋白，并通过免疫实验验证其激发特异性T细胞反应的能力，为癌症疫苗开发提供潜在靶点。
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**备注**：若需实际引用文献，建议通过PubMed/Google Scholar使用关键词“recombinant TCP11”或“human TCP11 function”检索最新研究，以上为模拟示例。
**Background of Recombinant Human TCP11 (T-Complex Protein 11)**
T-complex protein 11 (TCP11), also known as testis-enhanced gene transfer (TEGT) protein, is a member of the TCP-1 chaperonin family involved in protein folding and assembly. Initially identified for its role in spermatogenesis, TCP11 is highly expressed in the testis and localizes to the sperm acrosome, playing a critical role in acrosome formation and sperm-egg interaction during fertilization. Structurally, it contains conserved chaperonin-like domains, including ATP-binding motifs and regions facilitating substrate protein interactions.
Recombinant human TCP11 is engineered using genetic cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active protein for functional studies. Research highlights its importance in reproductive biology, particularly in understanding male infertility linked to TCP11 mutations or dysregulation. Beyond reproduction, TCP11 has been implicated in cancer, where its aberrant expression correlates with tumor progression, possibly through modulating cytoskeletal dynamics or stress-response pathways.
Studies leveraging recombinant TCP11 focus on elucidating its mechanistic roles in protein homeostasis, gamete development, and disease pathways, offering potential applications in diagnostics, fertility treatments, and targeted cancer therapies. Ongoing work aims to map its interactome and therapeutic relevance in cellular stress adaptation.
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