| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL31RA |
| Uniprot No | Q8NI17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-732aa |
| 氨基酸序列 | MMWTWALWMLPSLCKFSLAALPAKPENISCVYYYRKNLTCTWSPGKETSYTQYTVKRTYAFGEKHDNCTTNSSTSENRASCSFFLPRITIPDNYTIEVEAENGDGVIKSHMTYWRLENIAKTEPPKIFRVKPVLGIKRMIQIEWIKPELAPVSSDLKYTLRFRTVNSTSWMEVNFAKNRKDKNQTYNLTGLQPFTEYVIALRCAVKESKFWSDWSQEKMGMTEEEAPCGLELWRVLKPAEADGRRPVRLLWKKARGAPVLEKTLGYNIWYYPESNTNLTETMNTTNQQLELHLGGESFWVSMISYNSLGKSPVATLRIPAIQEKSFQCIEVMQACVAEDQLVVKWQSSALDVNTWMIEWFPDVDSEPTTLSWESVSQATNWTIQQDKLKPFWCYNISVYPMLHDKVGEPYSIQAYAKEGVPSEGPETKVENIGVKTVTITWKEIPKSERKGIICNYTIFYQAEGGKGFSKTVNSSILQYGLESLKRKTSYIVQVMASTSAGGTNGTSINFKTLSFSVFEIILITSLIGGGLLILIILTVAYGLKKPNKLTHLCWPTVPNPAESSIATWHGDDFKDKLNLKESDDSVNTEDRILKPCSTPSDKLVIDKLVVNFGNVLQEIFTDEARTGQENNLGGEKNGYVTCPFRPDCPLGKSFEELPVSPEIPPRKSQYLRSRMPEGTRPEAKEQLLFSGQSLVPDHLCEEGAPNPYLKNSVTAREFLVSEKLPEHTKGEV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"IL-31 and IL-31RA: Role in Allergic Disease and Chronic Pruritus"**
*Authors: Sonkoly E, et al.*
摘要：探讨IL-31与其受体IL31RA在特应性皮炎中的作用，通过重组蛋白实验证明IL-31/IL31RA信号通路介导瘙痒反应，并揭示其作为治疗靶点的潜力。
2. **"Structural and Functional Characterization of IL-31 Receptor Complex"**
*Authors: Zhang Q, et al.*
摘要：通过重组IL31RA和OSMRβ蛋白解析IL-31受体复合物的晶体结构，阐明配体结合机制及下游JAK/STAT信号激活模式。
3. **"Recombinant IL-31RA Alleviates Pruritus in Murine Models of Atopic Dermatitis"**
*Authors: Nemoto O, et al.*
摘要：构建可溶性重组IL31RA蛋白(sIL31RA)，在动物模型中验证其通过阻断IL-31信号减轻皮肤炎症和瘙痒的效果。
4. **"IL-31RA Expression in Sensory Neurons Mediates Chronic Itch"**
*Authors: Cevikbas F, et al.*
摘要：利用基因敲除和重组蛋白技术，证明感觉神经元上的IL31RA直接参与IL-31诱导的慢性瘙痒，为神经-免疫互作机制提供依据。
**Background of IL31RA Recombinant Protein**
Interleukin-31 receptor alpha (IL31RA) is a transmembrane protein that forms a heterodimeric receptor complex with oncostatin M receptor beta (OSMRβ) to mediate signaling of interleukin-31 (IL-31), a cytokine implicated in inflammatory and immune responses. IL-31/IL31RA signaling is strongly associated with pruritus (itch) and skin inflammation, particularly in conditions like atopic dermatitis, allergic contact dermatitis, and prurigo nodularis. The IL31RA gene encodes a type I cytokine receptor with conserved structural motifs, including fibronectin type III domains and a WSXWS motif critical for ligand binding and signal transduction.
Recombinant IL31RA protein is engineered to mimic the extracellular domain of the native receptor, often produced in mammalian or insect expression systems to ensure proper post-translational modifications. This soluble form retains ligand-binding capacity and is utilized in research to study IL-31 signaling mechanisms, screen therapeutic inhibitors, or develop diagnostic tools. Its production typically involves cloning the IL31RA gene segment encoding the ectodomain into expression vectors, followed by purification via affinity tags (e.g., His-tag) and validation through ligand-binding assays (e.g., ELISA, SPR).
Therapeutic interest in IL31RA stems from its role as a druggable target for inflammatory skin diseases. Monoclonal antibodies or small molecules blocking IL-31/IL31RA interaction have shown promise in clinical trials, such as nemolizumab, an anti-IL31RA antibody reducing pruritus in atopic dermatitis. Recombinant IL31RA protein also serves as a decoy receptor to neutralize IL-31 activity *in vitro* or *in vivo*, aiding preclinical studies. Additionally, it supports biomarker discovery and mechanistic studies linking IL-31 to neuroimmune crosstalk, fibrosis, and cancer-associated itch.
In summary, IL31RA recombinant protein is a vital tool for elucidating IL-31 biology and advancing therapies for IL-31-driven pathologies.
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