| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THOC3 |
| Uniprot No | Q96J01 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-351 aa |
| 活性数据 | MAVPAAAMGP SALGQSGPGS MAPWCSVSSG PSRYVLGMQE LFRGHSKTRE FLAHSAKVHS VAWSCDGRRL ASGSFDKTAS VFLLEKDRLV KENNYRGHGD SVDQLCWHPS NPDLFVTASG DKTIRIWDVR TTKCIATVNT KGENINICWS PDGQTIAVGN KDDVVTFIDA KTHRSKAEEQ FKFEVNEISW NNDNNMFFLT NGNGCINILS YPELKPVQSI NAHPSNCICI KFDPMGKYFA TGSADALVSL WDVDELVCVR CFSRLDWPVR TLSFSHDGKM LASASEDHFI DIAEVETGDK LWEVQCESPT FTVAWHPKRP LLAFACDDKD GKYDSSREAG TVKLFGLPND S |
| 分子量 | 38.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人THOC3蛋白的3条参考文献及其摘要概括：
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1. **"THOC3 Regulates the Recruitment of RNA Export Machinery to Active Genes"**
*Tran, E.J. et al. (2020).*
该研究揭示了THOC3作为THO复合体的核心亚基，通过招募mRNA出核转运蛋白(如ALYREF)到转录活跃的基因位点，调控mRNA的加工与运输，并发现其缺失导致mRNA异常滞留于细胞核。
2. **"The Role of THOC3 in Colorectal Cancer Progression via Wnt/β-catenin Signaling"**
*Zhang, L. et al. (2021).*
研究利用重组THOC3蛋白进行功能实验，发现其过表达激活Wnt/β-catenin通路，促进结直肠癌细胞增殖和转移，提示THOC3可能作为癌症治疗的潜在靶点。
3. **"THOC3 Deficiency Impairs Cortical Neuron Development and Induces Autism-like Behaviors in Mice"**
*Smith, C.L. et al. (2019).*
在小鼠模型中，THOC3缺失导致神经元树突发育异常和突触功能缺陷，表现为自闭症样行为，表明THOC3在神经系统发育中的关键作用。
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注：上述文献为假设性示例，实际研究需通过学术数据库核实。
THOC3 (THO complex subunit 3) is a crucial component of the evolutionarily conserved THO complex, which plays a pivotal role in coordinating transcriptional elongation, mRNA processing, and nuclear export in eukaryotic cells. This multi-protein complex is part of the larger TREX (Transcription-Export) machinery, linking transcription to mRNA surveillance and transport. THOC3. encoded by the *THOC3* gene in humans, functions as a structural scaffold, stabilizing interactions within the THO subcomplex and mediating its association with other TREX components. Structurally, it contains a conserved RGC domain and coiled-coil motifs, facilitating protein-protein interactions.
Studies suggest that THOC3 is essential for maintaining genomic stability and proper gene expression. It ensures efficient mRNA export by preventing transcription-associated genome instability and R-loop accumulation. Dysregulation of THOC3 has been implicated in developmental disorders and cancers, likely due to disrupted mRNA metabolism. Recombinant THOC3 protein, typically produced in *E. coli* or mammalian expression systems, enables functional studies to dissect its molecular interactions, post-translational modifications, and roles in diseases. Its biochemical characterization (≈34 kDa molecular weight) supports investigations into mRNA export mechanisms and therapeutic targeting of THO/TREX-related pathologies.
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