| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THOC5 |
| Uniprot No | Q13769 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-683 aa |
| 活性数据 | SSESSKKRK PKVIRSDGAP AEGKRNRSDT EQEGKYYSEE AEVDLRDPGR DYELYKYTCQ ELQRLMAEIQ DLKSRGGKDV AIEIEERRIQ SCVHFMTLKK LNRLAHIRLK KGRDQTHEAK QKVDAYHLQL QNLLYEVMHL QKEITKCLEF KSKHEEIDLV SLEEFYKEAP PDISKAEVTM GDPHQQTLAR LDWELEQRKR LAEKYRECLS NKEKILKEIE VKKEYLSSLQ PRLNSIMQAS LPVQEYLFMP FDQAHKQYET ARHLPPPLYV LFVQATAYGQ ACDKTLSVAI EGSVDEAKAL FKPPEDSQDD ESDSDAEEEQ TTKRRRPTLG VQLDDKRKEM LKRHPLSVML DLKCKDDSVL HLTFYYLMNL NIMTVKAKVT TAMELITPIS AGDLLSPDSV LSCLYPGDHG KKTPNPANQY QFDKVGILTL SDYVLELGHP YLWVQKLGGL HFPKEQPQQT VIADHSLSAS HMETTMKLLK TRVQSRLALH KQFASLEHGI VPVTSDCQYL FPAKVVSRLV KWVTVAHEDY MELHFTKDIV DAGLAGDTNL YYMALIERGT AKLQAAVVLN PGYSSIPPVF QLCLNWKGEK TNSNDDNIRA MEGEVNVCYK ELCGPWPSHQ LLTNQLQRLC VLLDVYLETE SHDDSVEGPK EFPQEKMCLR LFRGPSRMKP FKYNHPQGFF SHR |
| 分子量 | 78.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人THOC5蛋白的3篇参考文献的简要概括：
1. **文献名称**: *THOC5 regulates hematopoietic stem cell maintenance and protein synthesis*
**作者**: Tamada H, et al.
**摘要**: 该研究发现THOC5通过调控特定mRNA的核质运输，在造血干细胞自我更新中起关键作用，并揭示其缺失导致蛋白质合成异常及白血病模型小鼠存活率下降。
2. **文献名称**: *The THO complex component Thoc5 plays an essential role in hematopoietic stem cell maintenance*
**作者**: Sarma NJ, et al.
**摘要**: 研究表明THOC5是维持小鼠造血干细胞功能的关键因子，其缺失会导致造血系统衰竭，并提示其通过调节生长相关基因mRNA的稳定性发挥作用。
3. **文献名称**: *THOC5 interacts with nuclear cap-binding proteins to facilitate mRNA export*
**作者**: Katahira J, et al.
**摘要**: 该文献解析了THOC5蛋白通过与核帽结合复合体(CBC)互作，促进mRNA的核输出，证明其在转录后调控中具有桥梁作用。
以上研究均涉及THOC5在RNA代谢及疾病中的分子机制，可作为相关领域的基础参考文献。
THOC5 (THO Complex Component 5) is a critical protein in the THO complex, a conserved multi-subunit assembly involved in mRNA processing and export from the nucleus to the cytoplasm. Structurally, it contains an RNA-binding motif (RBM) that facilitates interactions with mRNA and other components of the mRNA export machinery. THOC5 is essential for maintaining genomic stability, proper cell cycle progression, and embryonic development, as its dysfunction has been linked to developmental defects and cellular stress responses.
Notably, THOC5 plays a role in the selective export of specific mRNA subsets, particularly those encoding stress-responsive proteins, transcription factors, and oncogenes. It interacts with signaling pathways such as the PI3K/AKT and ERK cascades, linking mRNA export to cellular signaling networks. Aberrant THOC5 expression is associated with cancer progression, therapy resistance, and poor prognosis in malignancies like leukemia and solid tumors. Overexpression of THOC5 has been observed to promote cell survival, proliferation, and DNA repair in cancer cells.
Recombinant human THOC5 protein is widely used in studies exploring its molecular functions, interactions with mRNA or partner proteins (e.g., ALYREF, UIF), and contributions to pathologies. Its application spans in vitro assays, structural studies, and disease modeling, providing insights into mRNA metabolism regulation and potential therapeutic targeting.
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