| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMb1 |
| Uniprot No | P07942 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1533-1782aa |
| 氨基酸序列 | MPSTPQQLQNLTEDIRERVESLSQVEVILQHSAADIARAEMLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIESETAASEETLFNASQRISELERNVEELKRKAAQNSGEAEYIEKVVYTVKQSAEDVKKTLDGELDEKYKKVENLIAKKTEESADARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRSLLKDISQKVAVY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAMB1(层粘连蛋白β1链)重组蛋白研究的示例参考文献(注：文献信息为示例，建议通过学术数据库检索真实文献)：
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1. **文献名称**: "Recombinant LAMB1 promotes epithelial cell adhesion and suppresses tumor metastasis"
**作者**: Smith A, et al.
**摘要**: 研究利用哺乳动物细胞表达系统成功制备重组LAMB1蛋白，证实其通过调控整合素信号通路抑制癌细胞迁移，在体外模型中降低肿瘤侵袭能力。
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2. **文献名称**: "Structural and functional analysis of laminin β1 subunit in neural regeneration"
**作者**: Chen L, et al.
**摘要**: 通过大肠杆菌表达系统获得高纯度重组LAMB1片段，发现其能促进神经元轴突生长，为神经损伤修复提供潜在分子工具。
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3. **文献名称**: "LAMB1 recombinant protein enhances stem cell pluripotency in 3D culture systems"
**作者**: Tanaka K, et al.
**摘要**: 开发基于重组LAMB1的仿生细胞培养基质，证明其可维持多能干细胞自我更新，优于传统培养体系，应用于类器官构建。
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4. **文献名称**: "Interaction between recombinant LAMB1 and α6β4 integrin in diabetic wound healing"
**作者**: Gupta R, et al.
**摘要**: 研究重组LAMB1与整合素α6β4的相互作用机制，发现其通过激活FAK通路加速糖尿病小鼠模型皮肤创面愈合。
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建议通过PubMed、Google Scholar等平台以关键词"recombinant LAMB1 protein"或"laminin beta1 subunit recombinant"检索最新文献。
Laminin beta-1 (LAMb1) is a critical subunit of laminins, a family of high-molecular-weight glycoproteins that are fundamental components of the extracellular matrix (ECM). Laminins play essential roles in maintaining tissue architecture, cellular adhesion, differentiation, and signaling. As a β-chain subunit, LAMb1 combines with α and γ subunits to form trimeric laminin isoforms (e.g., laminin-111. laminin-521), which are pivotal in basement membrane formation and tissue development. These heterotrimers interact with cell surface receptors such as integrins and dystroglycans, mediating cell-ECM communication and influencing processes like cell migration, proliferation, and survival.
Recombinant LAMb1 proteins are engineered using biotechnological platforms (e.g., mammalian expression systems) to produce purified, functional subunits for research and therapeutic applications. These proteins retain key structural domains, including binding sites for ECM components and receptors, enabling their use in studying laminin assembly, cell behavior, and disease mechanisms. For instance, LAMb1 dysfunction is linked to pathologies like muscular dystrophy, kidney disorders, and cancer metastasis, making the recombinant protein a valuable tool for modeling diseases or screening therapeutics.
In regenerative medicine, recombinant LAMb1 is explored for coating synthetic scaffolds to enhance biocompatibility or promote stem cell adhesion in tissue engineering. Its role in neuronal development has also spurred interest in neural repair strategies. Compared to native laminins, recombinant forms offer better batch consistency, reduced immunogenicity, and customizable modifications (e.g., tagged versions for tracking). Ongoing research continues to uncover its potential in 3D cell culture systems, organoid development, and targeted drug delivery, underscoring its versatility in bridging structural biology and translational medicine.
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