| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DOCK4 |
| Uniprot No | Q8N1I0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOCK4重组蛋白的3篇参考文献，涵盖结构、功能及疾病相关研究：
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1. **标题**：*Structural insights into the DOCK4 protein and its interaction with Rho GTPases*
**作者**：Smith A, et al.
**摘要**：通过X射线晶体学解析了DOCK4重组蛋白的C端结构域，揭示了其与Rho家族GTP酶(如Cdc42)结合的分子机制，为DOCK4调控细胞骨架重组的机制提供结构基础。
2. **标题**：*DOCK4 regulates neuronal development via recombinant protein-mediated signaling pathways*
**作者**：Chen L, et al.
**摘要**：研究利用重组DOCK4蛋白在体外神经元模型中的功能实验，证明其通过激活Rac1信号通路促进轴突生长，并发现DOCK4缺失导致突触形成异常。
3. **标题**：*DOCK4重组蛋白在乳腺癌转移中的功能及临床意义*
**作者**：Wang Y, et al.
**摘要**：通过表达纯化的人源DOCK4重组蛋白，发现其抑制乳腺癌细胞迁移和侵袭，机制涉及下调EMT通路，且DOCK4低表达与患者预后不良相关。
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注：以上文献为示例，实际研究中建议通过PubMed或Web of Science以“DOCK4 recombinant protein”等关键词检索最新论文。部分研究可能需结合DOCK家族其他成员(如DOCK1/DOCK2)的功能类推。
DOCK4 (Dedicator of Cytokinesis 4) is a member of the DOCK protein family, which functions as guanine nucleotide exchange factors (GEFs) for Rho GTPases, a class of signaling molecules regulating cytoskeletal dynamics, cell migration, and intracellular trafficking. Specifically, DOCK4 activates Rac1 and/or Cdc42 by catalyzing the exchange of GDP for GTP, thereby modulating downstream pathways involved in cell adhesion, polarity, and membrane protrusion. The DOCK family is characterized by the presence of a conserved DOCKER (or CZH2) domain that mediates interactions with GTPases, alongside other structural motifs such as SH3 domains and potential membrane-binding regions.
DOCK4 has garnered attention for its role in developmental and pathological processes. Studies link it to neurodevelopment, where it influences axon guidance and synaptic plasticity, and cancer, where dysregulation contributes to tumor invasion and metastasis. For instance, DOCK4 interacts with the ELMO (Engulfment and Cell Motility) protein complex to promote Rac1 activation, facilitating cell migration in cancer cells. Genetic alterations in the DOCK4 locus, including deletions or mutations, have been associated with neurodevelopmental disorders (e.g., autism spectrum disorders) and certain cancers, highlighting its clinical relevance.
Recombinant DOCK4 protein, produced via heterologous expression systems (e.g., E. coli, mammalian cells), enables functional and structural studies. Its purified form is utilized to investigate enzymatic activity, protein-protein interactions (e.g., with GTPases or ELMO), and signaling mechanisms in vitro. Additionally, recombinant DOCK4 serves as a tool for drug discovery, particularly in targeting aberrant Rho GTPase signaling in diseases. Despite progress, the precise regulatory mechanisms and tissue-specific roles of DOCK4 remain under active exploration, emphasizing its complexity in cellular signaling networks.
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