| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKCd |
| Uniprot No | Q05655 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-676aa |
| 氨基酸序列 | MAPFLRIAFNSYELGSLQAEDEANQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDVDCKQSMRSEDEAKFPTMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTIFQKERFNIDMPHRFKVHNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLCGINQKLLAEALNQVTQRASRRSDSASSEPVGIYQGFEKKTGVAGEDMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED |
| 预测分子量 | 81.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PKCδ(蛋白激酶C δ型)重组蛋白的参考文献，包含文献名称、作者及摘要概述：
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1. **文献名称**：*"Expression and purification of active protein kinase Cδ using a baculovirus system"*
**作者**：Kiley SC, Parker PJ
**摘要**：该研究利用杆状病毒表达系统在昆虫细胞中成功表达并纯化出具有催化活性的重组PKCδ蛋白。通过体外激酶实验验证其磷酸化底物能力，为后续PKCδ功能研究提供可靠工具。
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2. **文献名称**：*"Structural insights into the regulatory mechanism of PKCδ by its C2 domain"*
**作者**：Leventis PA, Chou WH, Gagnon J
**摘要**：本研究通过重组表达人源PKCδ的C2结构域，结合X射线晶体学解析其三维结构，揭示了钙离子依赖性膜结合的结构基础，阐明该结构域在PKCδ激活中的调控作用。
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3. **文献名称**：*"Recombinant PKCδ induces apoptosis in cancer cells through caspase-3 activation"*
**作者**：Reyland ME, Anderson SM
**摘要**：作者在大肠杆菌中表达并纯化重组PKCδ蛋白，发现其过表达能直接激活caspase-3通路，诱导肿瘤细胞凋亡，提示PKCδ可作为癌症治疗的潜在靶点。
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这些文献分别从重组蛋白制备、结构功能解析及疾病机制角度探讨了PKCδ的作用。如需具体文献链接或补充更多研究，可进一步提供关键词或研究方向。
Protein kinase C delta (PKCδ), a member of the serine/threonine-specific protein kinase C (PKC) family, plays critical roles in cellular signaling pathways regulating apoptosis, proliferation, differentiation, and stress responses. Belonging to the novel PKC subfamily (nPKC), PKCδ requires diacylglycerol (DAG) and phospholipids for activation but is independent of calcium signaling. Structurally, it contains a regulatory domain with tandem C1 domains for DAG/membrane binding and a catalytic domain with kinase activity. Dysregulation of PKCδ is implicated in cancer, neurodegenerative disorders, and immune diseases, making it a therapeutic target of interest.
Recombinant PKCδ proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional enzymes for research and drug discovery. These proteins retain key post-translational modifications (e.g., phosphorylation) when expressed in eukaryotic systems, ensuring biological activity. Tags like His or GST are often added for purification and detection. Researchers use recombinant PKCδ to study its role in pathways such as DNA damage response, oxidative stress, and B-cell receptor signaling. For example, PKCδ activation promotes apoptosis in cancer cells, while its inhibition is explored in chronic lymphocytic leukemia. Additionally, structural studies using recombinant PKCδ aid in designing isoform-specific inhibitors, addressing challenges in PKC-targeted therapies due to family-wide homology. Its dual roles as a tumor suppressor or promoter, depending on context, underscore the need for precise mechanistic studies facilitated by recombinant tools.
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