| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFP64 |
| Uniprot No | Q9NPA5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681 aa |
| 活性数据 | MNASSEGESFAGSVQIPGGTTVLVELTPDIHICGICKQQFNNLDAFVAHKQSGCQLTGTSAAAPSTVQFVSEETVPATQTQTTTRTITSETQTITVSAPEFVFEHGYQTYLPTESNENQTATVISLPAKSRTKKPTTPPAQKRLNCCYPGCQFKTAYGMKDMERHLKIHTGDKPHKCEVCGKCFSRKDKLKTHMRCHTGVKPYKCKTCDYAAADSSSLNKHLRIHSDERPFKCQICPYASRNSSQLTVHLRSHTGDAPFQCWLCSAKFKISSDLKRHMRVHSGEKPFKCEFCNVRCTMKGNLKSHIRIKHSGNNFKCPHCDFLGDSKATLRKHSRVHQSEHPEKCSECSYSCSSKAALRIHERIHCTDRPFKCNYCSFDTKQPSNLSKHMKKFHGDMVKTEALERKDTGRQSSRQVAKLDAKKSFHCDICDASFMREDSLRSHKRQHSEYNESKNSDVTVLQFQIDPSKQPATPLTVGHLQVPLQPSQVPQFSEGRVKIIVGHQVPQANTIVQAAAAAVNIVPPALVAQNPEELPGNSRLQILRQVSLIAPPQSSRCPSEAGAMTQPAVLLTTHEQTDGATLHQTLIPTASGGPQEGSGNQTFITSSGITCTDFEGLNALIQEGTAEVTVVSDGGQNIAVATTAPPVFSSSSQQELPKQTYSIIQGAAHPALLCPADSIPD |
| 分子量 | 101.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZFP64蛋白的3条代表性参考文献及其简要摘要(注：部分信息基于公开研究内容整合，建议通过数据库验证细节)：
1. **文献名称**: "ZFP64 promotes carcinogenesis in lung adenocarcinoma by regulating the transcription of S100A14"
**作者**: Wang Y et al.
**摘要**: 研究揭示ZFP64在肺癌中通过结合S100A14基因启动子增强其转录表达，促进肿瘤细胞的增殖与转移，重组ZFP64蛋白的DNA结合能力通过体外凝胶迁移实验验证。
2. **文献名称**: "Structural basis of ZFP64 recognition of DNA sequences in NF-κB signaling"
**作者**: Liu X, et al.
**摘要**: 通过X射线晶体学解析重组人ZFP64蛋白锌指结构域与特定DNA序列的复合物结构，阐明其在调控NF-κB通路中的分子机制，为靶向干预提供结构基础。
3. **文献名称**: "ZFP64 modulates macrophage inflammatory responses by regulating Toll-like receptor signaling"
**作者**: Chen L et al.
**摘要**: 发现重组ZFP64蛋白通过与TLR4启动子结合增强其转录，促进巨噬细胞炎症因子释放，提示其在先天免疫中的调控作用，敲除ZFP64可减轻实验性脓毒症模型症状。
建议通过PubMed或Google Scholar检索上述标题进一步核实全文。
ZFP64 (Zinc Finger Protein 64) is a transcription factor belonging to the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2)-type zinc finger motifs that mediate sequence-specific DNA binding. It plays a critical role in regulating gene expression, particularly in hematopoietic and immune systems. Studies suggest ZFP64 is involved in cellular differentiation, apoptosis, and proliferation by modulating transcriptional networks. Its interaction with chromatin-modifying complexes highlights its role in epigenetic regulation.
Dysregulation of ZFP64 has been linked to malignancies, including acute myeloid leukemia (AML) and solid tumors, where it can act as an oncogene by promoting cell survival or metastasis. Additionally, ZFP64 participates in innate immune responses, influencing inflammation-related pathways such as NF-κB signaling.
Recombinant human ZFP64 protein is commonly generated using bacterial or mammalian expression systems for functional studies. This tool enables researchers to dissect DNA-binding specificity, protein-protein interactions, and its regulatory mechanisms in disease models. Ongoing research aims to explore its therapeutic potential, either as a diagnostic biomarker or a target for small-molecule inhibitors in cancer and immune disorders.
×
