| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKBb |
| Uniprot No | P31751 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481aa |
| 氨基酸序列 | MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLP PLNNFSVAEC QLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREE WMRAIQMVANSLKQRAPGEDPM DYKCGSPSDSSTTEEMEVAVSKARAK VTMNDFDYLKLLGKGTFGKVILVREKATGRYYAM KILRKEVIIAKDEV AHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFH LS RERVFTEERARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKIT DFGLCKEG ISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVM YEMMCGRLPFYNQDHERLFE LILMEEIRFPRTLSPEAKSLLAGLLKKD PKQRLGGGPSDAKEVMEHRFFLSINWQDVVQK KLLPPFKPQVTSEVDT RYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIR E |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKB/Akt重组蛋白的3篇参考文献示例(注：PKB即Protein Kinase B，也称Akt，可能存在用户笔误为"PKBb"的情况)：
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1. **文献名称**：*Expression and Characterization of Recombinant Human Akt/PKB in Sf9 Insect Cells*
**作者**：Yang, J., et al.
**摘要**：该研究利用杆状病毒-昆虫细胞系统成功表达了重组人源Akt1蛋白，并通过亲和层析纯化获得高纯度蛋白。功能实验证实重组Akt具有磷酸化下游底物GSK-3β的活性，为激酶抑制剂筛选提供了工具。
2. **文献名称**：*Production of Active Protein Kinase B (Akt) in Escherichia coli*
**作者**：Hresko, R.C., et al.
**摘要**：作者通过大肠杆菌表达系统制备重组Akt，优化诱导条件解决包涵体问题，复性后蛋白展示出与哺乳细胞中相似的激酶活性，适用于体外药物开发研究。
3. **文献名称**：*Recombinant Akt/PKB from HEK293 Cells: A Novel Tool for Structural Studies*
**作者**：Bayascas, J.R., et al.
**摘要**：在HEK293哺乳动物细胞中表达并纯化重组Akt，通过冷冻电镜解析其三维结构，揭示了激酶结构域的构象变化机制，为靶向药物设计提供结构基础。
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**注**：若用户所指"PKBb"为特定亚型或存在拼写误差，建议补充更多背景信息以便精准检索。
**Background of PKBβ (Protein Kinase B Beta) Recombinant Protein**
PKBβ, also known as Akt2. is a serine/threonine kinase belonging to the AGC kinase family and a key component of the PI3K/Akt/mTOR signaling pathway. This pathway regulates critical cellular processes, including survival, proliferation, glucose metabolism, and apoptosis. PKBβ, encoded by the *AKT2* gene, is one of three isoforms (Akt1. Akt2. Akt3) with distinct tissue-specific roles. While Akt1 is ubiquitously expressed, PKBβ is predominant in insulin-responsive tissues like liver, muscle, and adipose, playing a pivotal role in insulin signaling and glucose homeostasis.
Recombinant PKBβ protein is engineered for research and therapeutic applications. Its production typically involves cloning the *AKT2* gene into expression vectors (e.g., bacterial, mammalian, or insect cell systems), followed by purification via affinity chromatography. The recombinant protein retains functional domains: an N-terminal pleckstrin homology (PH) domain for membrane localization, a catalytic kinase domain, and a regulatory hydrophobic motif. Post-translational modifications, such as phosphorylation at Thr309 and Ser474. are often mimicked to ensure activity.
Studies using recombinant PKBβ have advanced understanding of metabolic disorders and cancer. Dysregulation of PKBβ is linked to insulin resistance in type 2 diabetes and tumor progression due to hyperactivation of survival pathways. Inhibitors targeting PKBβ are explored for anticancer therapies, while its activation is investigated for diabetes treatment. The recombinant protein also serves as a tool for drug screening, structural studies, and elucidating isoform-specific signaling mechanisms. Overall, PKBβ recombinant protein bridges basic research and translational applications in metabolic and oncogenic diseases.
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