| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFYVE16 |
| Uniprot No | Q7Z3T8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-809 aa |
| 活性数据 | MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQECVNSCASSETSYGTNESSLNKKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMGRCSKPICDLISDMGNLVHATNSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDHDSDTVREQQNDTSSELQNREIGGIKELGIKVDTTLSDSYNYSGTENLKDKKIFNQLESIVDFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKEEVDVAVITAAECLKEEGKTSALTCSLPKNEDLCLNDSNSRDENFKLPDFSFQEDKTVIKQSAQEDSKSLDLKDNDVIQDSSSALHVSSKDVPSSLSCLPASGSMCGSLIESKARGDFLPQHEHKDNIQDAVTIHEEIQNSVVLGGEPFKENDLLKQEKCKSILLQSLIEGMEDRKIDPDQTVIRAESLDGGDTSSTVVESQEGLSGTHVPESSDCCEGFINTFSSNDMDGQDLDYFNIDEGAKSGPLISDAELDAFLTEQYLQTTNIKSFEENVNDSKSQMNQIDMKGLDDGNINNIYFNAEAGAIGESHGINIICETVDKQNTIENGLSLGEKSTIPVQQGLPTSKSEITNQLSVSDINSQSVGGARPKQLFSLPSRTRSSKDLNKPDVPDTIESEPSTADTVVPITCAIDSTADPQVSFNSNYIDIESNSEGGSSFVTANEDSVPENTCKEGLVLGQKQPTWVPDSEAPNCMNCQVKFTFTKRRHHCRACGKVFCGVCCNRKCKLQYLEKEARVCVVCYETISKGEC |
| 分子量 | 114.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZFYVE16蛋白的3篇参考文献及其摘要内容：
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1. **文献名称**: *ZFYVE16 regulates autophagy by mediating phosphatidylinositol 3-phosphate synthesis from endosomes*
**作者**: Zhang Y et al.
**摘要**: 该研究解析了ZFYVE16蛋白在自噬体形成中的作用，发现其通过结合内体膜上的磷脂酰肌醇3-磷酸(PI3P)，调控自噬相关蛋白的募集。重组人ZFYVE16的体外实验表明，其C端结构域突变会显著抑制自噬通量。
2. **文献名称**: *The role of ZFYVE16 in cancer cell migration and invasion through endosomal trafficking*
**作者**: López-Domènech S et al.
**摘要**: 研究表明，ZFYVE16在多种癌细胞中高表达，并通过调控内吞运输途径影响细胞迁移。利用重组ZFYVE16蛋白的功能实验证实，其缺失会破坏EGFR等生长因子受体的回收，从而抑制肿瘤侵袭。
3. **文献名称**: *ZFYVE16 modulates neurodegeneration via selective autophagy pathways*
**作者**: Chen L et al.
**摘要**: 文章探讨了ZFYVE16在神经退行性疾病中的功能，发现其通过促进病理性蛋白聚集体的自噬性降解发挥保护作用。重组ZFYVE16的过表达可显著减少神经元中的毒性蛋白沉积。
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这些文献涵盖了ZFYVE16在自噬、癌症及神经退行性疾病中的分子机制研究，具体方法涉及重组蛋白的体外功能验证及疾病模型分析。
**Background of Recombinant Human ZFYVE16 Protein**
ZFYVE16 (Zinc Finger FYVE-Type Containing 16), also known as Endofin or Hrs-FYVE, is a ubiquitously expressed protein that plays a critical role in intracellular membrane trafficking, signal transduction, and autophagy regulation. It belongs to the FYVE domain protein family, characterized by a conserved FYVE domain that binds phosphatidylinositol 3-phosphate (PI3P) on endosomal membranes. This interaction facilitates its localization to early endosomes, where it mediates vesicle tethering and cargo sorting.
ZFYVE16 functions as a scaffold protein, interacting with components of the endosomal sorting complex required for transport (ESCRT) machinery and regulating epidermal growth factor receptor (EGFR) degradation. It also modulates transforming growth factor-β (TGF-β) signaling by influencing Smad4 trafficking and activity. Dysregulation of ZFYVE16 has been implicated in cancer progression, immune response, and neurodegenerative disorders.
Recombinant human ZFYVE16 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for studying its molecular mechanisms, structural interactions, and therapeutic potential. Its applications span in vitro binding assays, enzymatic studies, and pathway analysis, providing insights into cellular homeostasis and disease pathogenesis.
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