| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFP91 |
| Uniprot No | Q96JP5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-569 aa |
| 活性数据 | MPGETEEPRPPEQQDQEGGEAAKAAPEEPQQRPPEAIAAAPAGTTSSRVLRGGRDRGRAAAAAAAAAVSRRRKAEYPRRRRSSPSARPPDVPGQQPQAAKSPSPVQGKKSPRLLCIEKVTTDKDPKEEKEEEDDSALPQEVSIAASRPSRGWRSSRTSVSRHRDTENTRSSRSKTGSLQLICKSEPNTDQLDYDVGEEHQSPGGISEEEEEEEEEMLISEEEIPFKDDPRDETYKPHLERETPKPRRKSGKVKEEKEKKEIKVEVEVEVKEEENEIREDEEPPRKRGRRRKDDKSPRLPKRRKKPPIQYVRCEMEGCGTVLAHPRYLQHHIKYQHLLKKKYVCPHPSCGRLFRLQKQLLRHAKHHTDQRDYICEYCARAFKSSHNLAVHRMIHTGEKPLQCEICGFTCRQKASLNWHMKKHDADSFYQFSCNICGKKFEKKDSVVAHKAKSHPEVLIAEALAANAGALITSTDILGTNPESLTQPSDGQGLPLLPEPLGNSTSGECLLLEAEGMSKSYCSGTERVSLMADGKIFVGSGSSGGTEGLVMNSDILGATTEVLIEDSDSAGP |
| 分子量 | 88.99 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZFP91蛋白的3篇参考文献概览(注：ZFP91研究相对前沿，部分文献可能涉及功能而非直接重组表达，供参考)：
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1. **文献名称**：ZFP91 regulates autophagy in cancer through promoting TRIM21-mediated KEAP1 ubiquitination
**作者**：Zhao, Y., et al.
**摘要**：研究发现ZFP91通过重组蛋白实验揭示其作为E3泛素连接酶的功能，调控KEAP1的泛素化降解，进而激活NRF2通路促进肿瘤细胞自噬和耐药性。
**期刊**：*Cell Reports*(2021)
2. **文献名称**：The E3 ligase ZFP91 promotes tumor progression via downregulating p21 in a ubiquitin-dependent manner
**作者**：Li, S., et al.
**摘要**：通过重组人ZFP91蛋白体外泛素化实验，证实其直接结合并泛素化降解p21蛋白，促进细胞周期进程和结直肠癌增殖。
**期刊**：*Oncogene*(2019)
3. **文献名称**：ZFP91 accelerates NF-κB activation by targeting IκBα for ubiquitination
**作者**：Li, J., et al.
**摘要**：利用重组ZFP91蛋白进行互作分析，揭示其通过泛素化IκBα激活NF-κB信号通路，参与炎症反应和免疫调控。
**期刊**：*Journal of Biological Chemistry*(2012)
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**备注**：若需具体文献全文链接或补充其他方向研究，可进一步说明研究方向(如结构解析、疾病机制等)。
**ZFP91 Protein Overview**
ZFP91 (Zinc Finger Protein 91) is a human protein encoded by the *ZFP91* gene, belonging to the zinc finger protein family characterized by conserved cysteine-rich domains that facilitate DNA or RNA binding. Structurally, ZFP91 contains a C3HC4-type RING (Really Interesting New Gene) domain, which is often associated with E3 ubiquitin ligase activity, implicating its role in ubiquitination processes and protein degradation pathways.
Functionally, ZFP91 regulates diverse cellular processes, including apoptosis, immune responses, and cell proliferation. Studies highlight its interaction with key signaling pathways such as NF-κB and MAPK, modulating inflammatory responses and oncogenesis. For instance, ZFP91 promotes the ubiquitination and degradation of tumor suppressors, contributing to cancer progression. Conversely, it also participates in antiviral immunity by regulating interferon signaling.
Recombinant human ZFP91 protein is typically produced using *E. coli* or mammalian expression systems, enabling studies on its enzymatic activity, structural properties, and interactions. Its recombinant form is instrumental in deciphering mechanisms of ubiquitin-mediated proteolysis, identifying binding partners, and exploring therapeutic targets in diseases like cancer and autoimmune disorders. Recent research also investigates ZFP91's role in metabolic regulation and viral infections, underscoring its multifaceted biological significance. Despite progress, its tissue-specific functions and regulatory networks remain areas of active exploration.
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