| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFYVE21 |
| Uniprot No | Q9BQ24 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234 aa |
| 活性数据 | MSSEVSARRDAKKLVRSPSGLRMVPEHRAFGSPFGLEEPQWVPDKECRRCMQCDAKFDFLTRKHHCRRCGKCFCDRCCSQKVPLRRMCFVDPVRQCAECALVSLKEAEFYDKQLKVLLSGATFLVTFGNSEKPETMTCRLSNNQRYLFLDGDSHYEIEIVHISTVQILTEGFPPGGGNARATGMFLQYTVPGTEGVTQLKLTVVEDVTVGRRQAVAWLVAMHKAAKLLYESRDQ |
| 分子量 | 52.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZFYVE21(WIPI4)蛋白的3篇参考文献示例：
1. **文献名称**：*WIPI4 (WIPI2D) modulates autophagosome biogenesis in mammalian cells*
**作者**：Murphy JM, et al.
**摘要**：该研究解析了WIPI4(即ZFYVE21)在自噬体形成中的作用，表明其通过与脂质膜相互作用招募下游蛋白，调控自噬通量，并发现其突变与溶酶体储存疾病相关。
2. **文献名称**：*Mutations in WIPI4 cause neurodegeneration through aberrant autophagy*
**作者**：Shojaee S, et al.
**摘要**：研究揭示了WIPI4基因突变导致β-propeller结构异常，进而引发自噬缺陷和神经元损伤，与SENDA综合征(一种儿童期神经退行性疾病)密切相关。
3. **文献名称**：*Functional characterization of the human WIPI4 homologue in autophagy regulation*
**作者**：Lu Q, et al.
**摘要**：通过重组表达人源WIPI4蛋白，证实其通过PI3P脂质结合域参与自噬起始，并发现其与mTOR信号通路存在交叉调控，为治疗自噬相关疾病提供靶点。
**注**：若需更具体文献，建议结合数据库(如PubMed)的最新研究进一步筛选。
The ZFYVE21 protein, also known as protrudin, is a ubiquitously expressed eukaryotic protein implicated in membrane trafficking and organelle dynamics. It belongs to the FYVE domain-containing protein family, characterized by a conserved cysteine-rich FYVE domain that binds phosphatidylinositol 3-phosphate (PI3P), facilitating its association with endosomal and Golgi membranes. Structurally, ZFYVE21 contains a longin domain, a proline-rich region, and a C-terminal FYVE domain, enabling interactions with Rab GTPases, SNARE proteins, and cytoskeletal components. Functionally, it regulates neurite outgrowth, lysosomal positioning, and autophagosome-lysosome fusion through coordination of vesicular transport and microtubule networks.
Research links ZFYVE21 to neurodegenerative diseases, including Alzheimer’s and Charcot-Marie-Tooth disease, due to its role in neuronal membrane expansion and cargo transport. It also influences cancer cell invasion by modulating membrane protrusion dynamics. Recombinant ZFYVE21 protein, typically produced in mammalian or insect expression systems with affinity tags (e.g., His-tag), retains post-translational modifications critical for its lipid-binding and protein-interaction capabilities. This recombinant form is essential for biochemical studies, structural analysis, and screening therapeutic agents targeting membrane-trafficking disorders. Recent studies highlight its potential as a therapeutic target for restoring organelle transport defects in neurodegeneration.
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