| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFYVE9 |
| Uniprot No | O95405 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-762 aa |
| 活性数据 | MENYFQAEAYNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPPSHRLSFNPTLASVNESAVSNESQPQLKVFSLAHSAPLTTEEEDHCANGQDCNLNPEIATMWIDENAVAEDQLIKRNYSWDDQCSAVEVGEKKCGNLACLPDEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQSLMDAFSCSLDNENRQTDQFSFSINESTEKDMNSEKQMDPLNRPKTEGRSVNHLCPTSSDSLASVCSPSQLKDDGSIGRDPSMSAITSLTVDSVISSQGTDGCPAVKKQENYIPDEDLTGKISSPRTDLGSPNSFSHMSEGILMKKEPAEESTTEESLRSGLPLLLKPDMPNGSGRNNDCERCSDCLVPNEVRADENEGYEHEETLGTTEFLNMTEHFSESQDMTNWKLTKLNEMNDSQVNEEKEKFLQISQPEDTNGDSGGQCVGLADAGLDLKGTCISESEECDFSTVIDTPAANYLSNGCDSYGMQDPGVSFVPKTLPSKEDSVTEEKEIEESKSECYSNIYEQRGNEATEGSGLLLNSTGDLMKKNYLHNFCSQVPSVLGQSSPKVVASLPSISVPFGGARPKQPSNLKLQIPKPLSDHLQNDFPANSGNNTKNKNDILGKAKLGENSATNVCSPSLGNISNVDTNGEHLESYEAEISTRPCLALAPDSPDNDLRAGQFGISARKPFTTLGEVAPVWVPDSQAPNCMKCEARFTFTKRRHHCRACGKVFCASCCSLKCKLLYMDRKEARVCVICHSVLMNGKY |
| 分子量 | 110.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZFYVE9(SARIP)蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*ZFYVE9 (SARIP) regulates endosomal trafficking to mediate TGF-β signaling*
**作者**：Hayes, S., et al.
**摘要**：研究揭示了ZFYVE9在TGF-β信号中的功能，通过重组蛋白实验证明其通过与Smad2/Smad7复合物相互作用，调控受体在内体中的运输，从而影响信号传导效率。
2. **文献名称**：*Expression and purification of recombinant human ZFYVE9 for structural studies*
**作者**：Hu, L., et al.
**摘要**：报道了在大肠杆菌中高效表达并纯化重组人ZFYVE9蛋白的方法，通过核磁共振(NMR)初步分析了其FYVE结构域的锌离子结合特性。
3. **文献名称**：*ZFYVE9 modulates vesicle fusion by bridging RAB11 and SNARE complexes*
**作者**：Miyauchi, K., et al.
**摘要**：利用重组ZFYVE9蛋白进行体外膜结合实验，发现其通过连接RAB11阳性囊泡与SNARE蛋白，促进内体膜融合，支持细胞极性迁移。
(注：以上文献为示例，实际引用请核对原始论文信息)
ZFYVE9 (Zinc Finger FYVE-Type Containing 9), also known as SARAF (Store-Operated Calcium Entry-Associated Regulatory Factor), is a transmembrane protein encoded by the ZFYVE9 gene in humans. It plays a critical role in regulating cellular calcium homeostasis, particularly through its interaction with STIM1 and ORAI1 proteins in store-operated calcium entry (SOCE) pathways. By modulating SOCE, ZFYVE9 influences diverse physiological processes, including immune response, muscle contraction, and neurotransmitter release. Additionally, it participates in membrane repair mechanisms by facilitating the removal of damaged plasma membrane regions.
The recombinant human ZFYVE9 protein is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography. Its structure includes a conserved FYVE domain, which binds phosphatidylinositol 3-phosphate, and a transmembrane domain critical for its function in endoplasmic reticulum-plasma membrane junctions. Researchers utilize recombinant ZFYVE9 to study calcium signaling abnormalities linked to cardiovascular diseases, immune disorders, and neurodegenerative conditions. Its role in cancer progression, particularly in metastasis and therapy resistance, is also under investigation. As a tool, it aids in elucidating molecular mechanisms of calcium-dependent cellular responses and potential therapeutic targeting.
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