| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZKSCAN5 |
| Uniprot No | Q9Y2L8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-839 aa |
| 活性数据 | MIMTESREVIDLDPPAETSQEQEDLFIVKVEEEDCTWMQEYNPPTFETFYQRFRHFQYHEASGPREALSQLRVLCCEWLRPELHTKEQILELLVLEQFLTILPEEFQPWVREHHPESGEEAVAVIENIQRELEERRQQIVACPDVLPRKMATPGAVQESCSPHPLTVDTQPEQAPQKPRLLEENALPVLQVPSLPLKDSQELTASLLSTGSQKLVKIEEVADVAVSFILEEWGHLDQSQKSLYRDDRKENYGSITSMGYESRDNMELIVKQISDDSESHWVAPEHTERSVPQDPDFAEVSDLKGMVQRWQVNPTVGKSRQNPSQKRDLDAITDISPKQSTHGERGHRCSDCGKFFLQASNFIQHRRIHTGEKPFKCGECGKSYNQRVHLTQHQRVHTGEKPYKCQVCGKAFRVSSHLVQHHSVHSGERPYGCNECGKNFGRHSHLIEHLKRHFREKSQRCSDKRSKNTKLSVKKKISEYSEADMELSGKTQRNVSQVQDFGEGCEFQGKLDRKQGIPMKEILGQPSSKRMNYSEVPYVHKKSSTGERPHKCNECGKSFIQSAHLIQHQRIHTGEKPFRCEECGKSYNQRVHLTQHQRVHTGEKPYTCPLCGKAFRVRSHLVQHQSVHSGERPFKCNECGKGFGRRSHLAGHLRLHSREKSHQCRECGEIFFQYVSLIEHQVLHMGQKNEKNGICEEAYSWNLTVIEDKKIELQEQPYQCDICGKAFGYSSDLIQHYRTHTAEKPYQCDICRENVGQCSHTKQHQKIYSSTKSHQCHECGRGFTLKSHLNQHQGIHTGEKPFQCKECGMNFSWSCSLFKHLRSHERTDPINTLSVEGSLL |
| 分子量 | 123.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZKSCAN5蛋白的模拟参考文献示例(仅供参考，非真实文献)：
---
1. **文献名称**：*ZKSCAN5 regulates chromatin remodeling and gene transcription via interaction with histone modifiers*
**作者**：Smith J, et al.
**摘要**：本研究报道了重组ZKSCAN5蛋白的体外表达与纯化，并发现其通过锌指结构域与组蛋白甲基转移酶相互作用，参与调控靶基因的染色质状态和转录活性。
---
2. **文献名称**：*Recombinant ZKSCAN5 promotes tumor cell proliferation through epigenetic regulation of oncogenes*
**作者**：Li X, et al.
**摘要**：通过构建重组ZKSCAN5蛋白，研究者发现其能够结合特定基因启动子区域，抑制肿瘤抑制因子的表达，从而促进结直肠癌细胞增殖，提示其在癌症中的潜在作用。
---
3. **文献名称**：*Structural analysis of the zinc finger domains in human ZKSCAN5 protein*
**作者**：Wang R, et al.
**摘要**：利用X射线晶体学解析了重组ZKSCAN5蛋白的锌指结构域三维结构，揭示了其与DNA结合的分子机制，为开发靶向转录因子的药物提供了结构基础。
---
**注**：以上文献为示例，实际文献需通过数据库(如PubMed、Web of Science)检索确认。
ZKSCAN5. also known as zinc finger with SCAN domain-containing protein 5. is a member of the SCAN family of transcription factors characterized by a conserved SCAN domain (SRE-ZBP, CTfin51. AW-1. and Number18). This domain mediates protein-protein interactions, particularly homodimerization or heterodimerization with other SCAN-containing proteins. The human ZKSCAN5 protein additionally contains multiple C2H2-type zinc finger motifs, enabling sequence-specific DNA binding to regulate target gene expression. It is implicated in transcriptional repression or activation, depending on cellular context and interacting partners.
Recombinant ZKSCAN5 protein is typically produced using expression systems like *E. coli* or mammalian cells, followed by affinity purification for functional studies. Its recombinant form facilitates investigations into its role in chromatin remodeling, cell differentiation, and disease pathways. Research highlights its involvement in cancer progression, where it modulates genes linked to proliferation and metastasis, and in neurodegenerative conditions. Notably, ZKSCAN5 may interact with epigenetic modifiers, suggesting its integration into broader regulatory networks. Studies using recombinant protein have also explored its potential as a diagnostic marker or therapeutic target. However, its precise biological mechanisms remain under active investigation, necessitating further structural and functional analyses to clarify its physiological and pathological significance.
×
