| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZIM2 |
| Uniprot No | Q9NZV7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-527 aa |
| 活性数据 | MYQPEDDNNSDVTSDDDMTRNRRESSPPHSVHSFSGDRDWDRRGRSRDMEPRDRWSHTRNPRSRMPPRDLSLPVVAKTSFEMDREDDRDSRAYESRSQDAESYQNVVDLAEDRKPHNTIQDNMENYRKLLSLGFLAQDSVPAEKRNTEMLDNLPSAGSQFPDFKHLGTFLVFEELVTFEDVLVDFSPEELSSLSAAQRNLYREVMLENYRNLVSLGHQFSKPDIISRLEEEESYAMETDSRHTVICQGESHDDPLEPHQGNQEKLLTPITMNDPKTLTPERSYGSDEFERSSNLSKQSKDPLGKDPQEGTAPGICTSPQSASQENKHNRCEFCKRTFSTQVALRRHERIHTGKKPYECKQCAEAFYLMPHLNRHQKTHSGRKTSGCNEGRKPSVQCANLCERVRIHSQEDYFECFQCGKAFLQNVHLLQHLKAHEAARVLPPGLSHSKTYLIRYQRKHDYVGERACQCCDCGRVFSRNSYLIQHYRTHTQERPYQCQLCGKCFGRPSYLTQHYQLHSQEKTVECDHC |
| 分子量 | 87.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZIM2蛋白的3篇示例参考文献(注：部分信息为虚构示例，实际文献需根据具体研究验证)：
1. **文献名称**：*ZIM2 regulates embryonic stem cell pluripotency via histone modification interactions*
**作者**：Li, X.; Zhang, Y.; Zhou, H.
**摘要**：本研究利用重组人ZIM2蛋白，揭示了其通过结合组蛋白甲基转移酶复合物(如PRC2)调控胚胎干细胞多能性的分子机制。通过体外重组表达ZIM2蛋白，证实其直接参与染色质重塑过程。
2. **文献名称**：*Expression and purification of recombinant human ZIM2 in a mammalian system for functional analysis*
**作者**：Wang, R.; Kim, J.; Tanaka, M.
**摘要**：开发了一种基于HEK293细胞的重组人ZIM2蛋白表达与纯化方法，并验证其与p53蛋白的相互作用，提示ZIM2在肿瘤抑制中的潜在功能。
3. **文献名称**：*Structural insights into the zinc finger domain of ZIM2 and its DNA-binding specificity*
**作者**：Gupta, S.; Müller, C.W.; Lee, S.
**摘要**：通过重组表达ZIM2蛋白的锌指结构域，结合X射线晶体学分析，阐明了ZIM2识别特定DNA序列的结构基础，为其在基因转录调控中的作用提供依据。
4. **文献名称**：*ZIM2 promotes osteogenic differentiation through BMP-Smad signaling*
**作者**：Chen, L.; Ito, S.; Park, K.
**摘要**：体外实验表明，重组人ZIM2蛋白通过增强BMP信号通路中Smad蛋白的磷酸化，正向调控间充质干细胞的成骨分化。
**注意**：以上文献为模拟示例，实际研究中需查询具体数据库(如PubMed、Web of Science)获取真实文献。
ZIM2 (Zinc Finger Imprinted 2) protein, encoded by the ZIM2 gene in humans, is a member of the Krüppel-associated box (KRAB) zinc finger protein family. It is a maternally expressed imprinted gene located within the human chromosome 19q13.43 imprinted cluster, which also includes the H19/IGF2 locus. ZIM2 contains a conserved C2H2-type zinc finger domain, suggesting its role as a transcription factor involved in DNA binding and gene regulation. Its imprinting status – preferential expression from the maternal allele – links it to epigenetic mechanisms governing development and cellular differentiation.
Studies associate ZIM2 with genomic imprinting disorders and cancer. Abnormal ZIM2 expression has been observed in certain tumors, potentially contributing to dysregulated cell proliferation. The protein may interact with chromatin-modifying complexes to mediate transcriptional repression, though its exact molecular targets remain under investigation. Recombinant ZIM2 protein is typically produced in bacterial (e.g., E. coli) or mammalian expression systems with epitope tags for purification and detection. This engineered protein enables functional studies, including DNA-binding assays, protein interaction analyses, and investigations into its role in imprinting control. Research on ZIM2 continues to explore its therapeutic potential as a biomarker or target in imprinting-related diseases and oncogenesis.
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