| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZHX2 |
| Uniprot No | Q9Y6X8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-837aa |
| 活性数据 | MASKRKSTTPCMVRTSQVVEQDVPEEVDRAKEKGIGTPQPDVAKDSWAAELENSSKENEVIEVKSMGESQSKKLQGGYECKYCPYSTQNLNEFTEHVDMQHPNVILNPLYVCAECNFTTKKYDSLSDHNSKFHPGEANFKLKLIKRNNQTVLEQSIETTNHVVSITTSGPGTGDSDSGISVSKTPIMKPGKPKADAKKVPKKPEEITPENHVEGTARLVTDTAEILSRLGGVELLQDTLGHVMPSVQLPPNINLVPKVPVPLNTTKYNSALDTNATMINSFNKFPYPTQAELSWLTAASKHPEEHIRIWFATQRLKHGISWSPEEVEEARKKMFNGTIQSVPPTITVLPAQLAPTKVTQPILQTALPCQILGQTSLVLTQVTSGSTTVSCSPITLAVAGVTNHGQKRPLVTPQAAPEPKRPHIAQVPEPPPKVANPPLTPASDRKKTKEQIAHLKASFLQSQFPDDAEVYRLIEVTGLARSEIKKWFSDHRYRCQRGIVHITSESLAKDQLAIAASRHGRTYHAYPDFAPQKFKEKTQGQVKILEDSFLKSSFPTQAELDRLRVETKLSRREIDSWFSERRKLRDSMEQAVLDSMGSGKKGQDVGAPNGALSRLDQLSGAQLTSSLPSPSPAIAKSQEQVHLLRSTFARTQWPTPQEYDQLAAKTGLVRTEIVRWFKENRCLLKTGTVKWMEQYQHQPMADDHGYDAVARKATKPMAESPKNGGDVVPQYYKDPKKLCEEDLEKLVTRVKVGSEPAKDCLPAKPSEATSDRSEGSSRDGQGSDENEESSVVDYVEVTVGEEDAISDRSDSWSQAAAEGVSELAESDSDCVPAEAGQA |
| 分子量 | 92,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**: "ZHX2 inhibits SREBP1c-mediated de novo lipogenesis by recruiting HDAC3 to the promoter"
**作者**: Zhang Y, et al.
**摘要**: 研究揭示了重组人ZHX2蛋白在调控脂质合成中的作用，通过招募组蛋白去acetyl酶HDAC3抑制SREBP1c的转录活性，降低脂肪生成相关基因表达，为代谢性疾病提供机制解释。
2. **文献名称**: "ZHX2 suppresses hepatocellular carcinoma initiation by repressing the transcription of TERT"
**作者**: Wang C, et al.
**摘要**: 本文利用重组ZHX2蛋白验证其直接结合人端粒酶逆转录酶(TERT)基因启动子，抑制肝细胞癌发生，提出ZHX2作为肝癌的潜在抑癌因子。
3. **文献名称**: "Structural and functional analysis of the ZHX2-DREAM complex in transcriptional repression"
**作者**: Li H, et al.
**摘要**: 通过重组ZHX2蛋白体外实验，解析其与DREAM复合物互作的分子机制，阐明ZHX2通过招募该复合物抑制细胞周期相关基因，参与肿瘤抑制通路。
4. **文献名称**: "Recombinant ZHX2 protein interactions with NF-YA regulate cancer stemness genes"
**作者**: Kim J, et al.
**摘要**: 研究利用重组ZHX2蛋白证明其与NF-YA形成复合物，抑制干细胞特性基因(如SOX2、OCT4)，为靶向肿瘤干细胞提供依据。
注：以上为虚构文献，实际研究中建议通过PubMed、Google Scholar等平台以“ZHX2 recombinant protein”或“ZHX2 function”等关键词检索真实文献。
Zinc fingers and homeoboxes protein 2 (ZHX2) is a member of the ZHX family, characterized by the presence of two zinc finger motifs and five homeodomains. First identified in the early 2000s, ZHX2 is a nuclear transcription repressor that regulates gene expression through interactions with DNA or protein partners. It forms heterodimers with ZHX1 or ZHX3 and often associates with transcriptional regulators like NF-YA to modulate target genes involved in cell proliferation, differentiation, and apoptosis. ZHX2 is widely expressed in tissues, with high levels observed in the liver, kidney, and brain.
Research highlights ZHX2’s dual role in cancer, acting as either a tumor suppressor or promoter depending on context. In hepatocellular carcinoma, ZHX2 downregulation correlates with tumor progression by derepressing oncogenes like c-Myc. Conversely, its overexpression in breast or prostate cancer may enhance metastasis. Beyond oncology, ZHX2 regulates metabolic pathways, including lipid metabolism, and is implicated in neurodevelopment and immune responses. Structural studies reveal its zinc fingers mediate DNA binding, while homeodomains facilitate protein interactions. Post-translational modifications, such as phosphorylation, fine-tune its activity.
Despite its functional diversity, ZHX2’s mechanistic details remain unclear, prompting ongoing studies to explore its diagnostic or therapeutic potential in diseases.
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