| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZHX1 |
| Uniprot No | Q96EF9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-873 aa |
| 活性数据 | MASRRKSTTPCMVLASEQDPDLELISDLDEGPPVLTPVENTRAESISSDEEVHESVDSDNQQNKKVEGGYECKYCTFQTPDLNMFTFHVDSEHPNVVLNSSYVCVECNFLTKRYDALSEHNLKYHPGEENFKLTMVKRNNQTIFEQTINDLTFDGSFVKEENAEQAESTEVSSSGISISKTPIMKMMKNKVENKRIAVHHNSVEDVPEEKENEIKPDREEIVENPSSSASESNTSTSIVNRIHPSTASTVVTPAAVLPGLAQVITAVSAQQNSNLIPKVLIPVNSIPTYNAALDNNPLLLNTYNKFPYPTMSEITVLSAQAKYTEEQIKIWFSAQRLKHGVSWTPEEVEEARRKQFNGTVHTVPQTITVIPTHISTGSNGLPSILQTCQIVGQPGLVLTQVAGTNTLPVTAPIALTVAGVPSQNNIQKSQVPAAQPTAETKPATAAVPTSQSVKHETALVNPDSFGIRAKKTKEQLAELKVSYLKNQFPHDSEIIRLMKITGLTKGEIKKWFSDTRYNQRNSKSNQCLHLNNDSSTTIIIDSSDETTESPTVGTAQPKQSWNPFPDFTPQKFKEKTAEQLRVLQASFLNSSVLTDEELNRLRAQTKLTRREIDAWFTEKKKSKALKEEKMEIDESNAGSSKEEAGETSPADESGAPKSGSTGKICKKTPEQLHMLKSAFVRTQWPSPEEYDKLAKESGLARTDIVSWFGDTRYAWKNGNLKWYYYYQSANSSSMNGLSSLRKRGRGRPKGRGRGRPRGRPRGSKRINNWDRGPSLIKFKTGTAILKDYYLKHKFLNEQDLDELVNKSHMGYEQVREWFAERQRRSELGIELFEENEEEDEVIDDQEEDEEETDDSDTWEPPRHVKRKLSKSDD |
| 分子量 | 124.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的重组人ZHX1蛋白相关参考文献示例(非真实文献)：
1. **ZHX1 inhibits hepatocellular carcinoma progression through epigenetic regulation of CDK4**
*作者：Li, X. et al.*
摘要：研究证实ZHX1通过结合CDK4基因启动子区域并招募组蛋白去乙酰化酶复合物，抑制肝癌细胞增殖和转移。
2. **Interplay between ZHX1 and NF-κB signaling in lung cancer chemoresistance**
*作者：Wang, Y. et al.*
摘要：发现ZHX1通过抑制NF-κB通路活性增强肺癌细胞对顺铂的敏感性，为靶向ZHX1的联合治疗提供依据。
3. **ZHX1 interacts with DNMT3A to regulate methylation-dependent tumor suppression**
*作者：Zhang, R. et al.*
摘要：基于Co-IP和ChIP-seq实验，揭示ZHX1与DNA甲基转移酶DNMT3A协同作用，通过基因组甲基化修饰抑制乳腺癌进展。
4. **ZHX1 acts as a transcriptional repressor by forming complexes with HDAC1/2 in gastric cancer**
*作者：Chen, H. et al.*
摘要：报道ZHX1通过与HDAC1/2互作下调原癌基因c-Myc的表达，抑制胃癌细胞侵袭并诱导凋亡。
*注：以上为模拟内容，实际文献需通过PubMed、Google Scholar等数据库检索。*
Zinc finger and homeodomain protein 1 (ZHX1) is a transcription regulatory protein belonging to the ZHX family, characterized by its unique combination of zinc finger motifs and homeodomains. First identified in the early 2000s, ZHX1 functions primarily as a transcriptional repressor, modulating gene expression through interactions with nuclear transcription factors, such as NF-YA, and chromatin-remodeling complexes. Structurally, it contains two N-terminal C2H2-type zinc finger domains critical for DNA binding and protein interactions, followed by five homeodomains that may stabilize DNA or protein interactions.
ZHX1 is widely expressed in human tissues, particularly in the liver, kidney, and brain. It plays roles in cellular processes like proliferation, differentiation, and apoptosis. Emerging studies link ZHX1 to tumorigenesis, acting as either an oncogene or tumor suppressor depending on context. For example, it suppresses hepatocellular carcinoma by inhibiting cell cycle genes but may promote renal cancer progression via dysregulated signaling pathways.
Epigenetic mechanisms, including promoter methylation and miRNA regulation (e.g., miR-155), influence ZHX1 expression. Recombinant human ZHX1 protein, typically produced in *E. coli* or mammalian systems, enables functional studies to dissect its molecular mechanisms, offering potential for therapeutic targeting. Its dual regulatory roles underscore complexity in transcriptional networks, driving ongoing research into tissue-specific functions and disease associations.
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