| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZHX3 |
| Uniprot No | Q9H4I2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-969 aa |
| 活性数据 | MASKRKSTTPCMIPVKTVVLQDASMEAQPAETLPEGPQQDLPPEASAASSEAAQNPSSTDGSTLANGHRSTLDGYLYSCKYCDFRSHDMTQFVGHMNSEHTDFNKDPTFVCSGCSFLAKTPEGLSLHNATCHSGEASFVWNVAKPDNHVVVEQSIPESTSTPDLAGEPSAEGADGQAEIIITKTPIMKIMKGKAEAKKIHTLKENVPSQPVGEALPKLSTGEMEVREGDHSFINGAVPVSQASASSAKNPHAANGPLIGTVPVLPAGIAQFLSLQQQPPVHAQHHVHQPLPTAKALPKVMIPLSSIPTYNAAMDSNSFLKNSFHKFPYPTKAELCYLTVVTKYPEEQLKIWFTAQRLKQGISWSPEEIEDARKKMFNTVIQSVPQPTITVLNTPLVASAGNVQHLIQAALPGHVVGQPEGTGGGLLVTQPLMANGLQATSSPLPLTVTSVPKQPGVAPINTVCSNTTSAVKVVNAAQSLLTACPSITSQAFLDASIYKNKKSHEQLSALKGSFCRNQFPGQSEVEHLTKVTGLSTREVRKWFSDRRYHCRNLKGSRAMIPGDHSSIIIDSVPEVSFSPSSKVPEVTCIPTTATLATHPSAKRQSWHQTPDFTPTKYKERAPEQLRALESSFAQNPLPLDEEMDRLRSETKMTRREIDSWFSERRKKVNAEETKKAEENASQEEEEAAEDEGGEEDLASELRVSGENGSLEMPSSHILAERKVSPIKINLKNLRVTEANGRNEIPGLGACDPEDDESNKLAEQLPGKVSCKKTAQQRHLLRQLFVQTQWPSNQDYDSIMAQTGLPRPEVVRWFGDSRYALKNGQLKWYEDYKRGNFPPGLLVIAPGNRELLQDYYMTHKMLYEEDLQNLCDKTQMSSQQKQTEFDLINVKDWPVWETACHVEEPNPTLCCHMPFPCPAAGHLGELPESSQTAQSLPLPSACPPPSKQQARWGSHQFFLPQCRTFPLPSNG |
| 分子量 | 132.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZHX3蛋白的3篇参考文献(模拟示例，非真实文献)：
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1. **文献名称**：*Structural Basis for ZHX3-Mediated Transcriptional Repression in Hepatocellular Carcinoma*
**作者**：Liu, X. et al.
**摘要**：本研究通过体外表达并纯化重组人ZHX3蛋白，解析了其锌指结构域与靶DNA结合的晶体结构。实验表明，ZHX3通过特异性识别DNA保守序列，招募HDAC复合物抑制基因转录，为肝癌中ZHX3的抑癌机制提供分子依据。
2. **文献名称**：*ZHX3 Suppresses Breast Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition*
**作者**：Wang, Y. et al.
**摘要**：通过哺乳动物细胞系表达重组ZHX3蛋白，研究发现其通过抑制SNAIL和TWIST等EMT相关基因的转录活性，阻断乳腺癌细胞侵袭和转移。体内实验证实，ZHX3过表达显著降低肿瘤肺转移发生率。
3. **文献名称**：*Recombinant ZHX3 Protein Attenuates Renal Fibrosis via Modulating TGF-β/Smad Signaling*
**作者**：Zhang, L. et al.
**摘要**：利用大肠杆菌表达系统制备高纯度重组ZHX3蛋白，发现其能直接结合Smad3蛋白，抑制TGF-β信号转导，减少肾纤维化模型小鼠的胶原沉积。该研究提出ZHX3作为肾脏疾病的潜在治疗靶点。
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以上内容基于领域内典型研究方向模拟生成，实际文献需通过学术数据库检索验证。
**Background of Recombinant Human ZHX3 Protein**
Zinc fingers and homeoboxes protein 3 (ZHX3), also known as HDRP, is a member of the ZHX family of transcriptional repressors, which includes ZHX1 and ZHX2. These proteins are characterized by N-terminal tandem C2H2-type zinc finger motifs and C-terminal homeodomains, enabling DNA binding and protein-protein interactions. ZHX3 is ubiquitously expressed in human tissues and plays regulatory roles in cell proliferation, differentiation, and tumorigenesis. It functions as a transcriptional repressor by interacting with nuclear transcription factors, such as NF-YA, and modulating the activity of target genes involved in cell cycle control and apoptosis.
Recombinant human ZHX3 protein is produced via *in vitro* expression systems (e.g., *E. coli* or mammalian cells) to study its structural and functional properties. Its recombinant form allows researchers to explore molecular mechanisms underlying ZHX3’s role in diseases, particularly cancers (e.g., hepatocellular carcinoma, breast cancer), where ZHX3 dysregulation is linked to tumor suppression or progression. Additionally, ZHX3 interacts with viral proteins, suggesting a role in viral pathogenesis. Studies using recombinant ZHX3 have also elucidated its homodimerization capability and post-translational modifications, which influence its stability and activity. This protein serves as a valuable tool for developing therapeutic strategies targeting ZHX3-associated pathways.
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