| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF132 |
| Uniprot No | P52740 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-706 aa |
| 活性数据 | MALPSPQVLMGLPALLMGPAQHTSWPCGSAVPTLKSMVTFEDVAVYFSQEEWELLDAAQR HLYHSVMLENLELVTSLGSWHGVEGEGAHPKQNVSVEVLQVRIPNADPSTKKANSCDMCG PFLKDILHLAEHQGTQSEEKPYTCGACGRDFWLNANLHQHQKEHSGGKPFRWYKDRDALM KSSKVHLSENPFTCREGGKVILGSCDLLQLQAVDSGQKPYSNLGQLPEVCTTQKLFECSN CGKAFLKSSTLPNHLRTHSEEIPFTCPTGGNFLEEKSILGNKKFHTGEIPHVCKECGKAF SHSSKLRKHQKFHTEVKYYECIACGKTFNHKLTFVHHQRIHSGERPYECDECGKAFSNRS HLIRHEKVHTGERPFECLKCGRAFSQSSNFLRHQKVHTQVRPYECSQCGKSFSRSSALIQ HWRVHTGERPYECSECGRAFNNNSNLAQHQKVHTGERPFECSECGRDFSQSSHLLRHQKV HTGERPFECCDCGKAFSNSSTLIQHQKVHTGQRPYECSECRKSFSRSSSLIQHWRIHTGE KPYECSECGKAFAHSSTLIEHWRVHTKERPYECNECGKFFSQNSILIKHQKVHTGEKPYK CSECGKFFSRKSSLICHWRVHTGERPYECSECGRAFSSNSHLVRHQRVHTQERPYECIQC GKAFSERSTLVRHQKVHTRERTYECSQCGKLFSHLCNLAQHKKIHT |
| 分子量 | 80.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人ZNF132蛋白的示例参考文献(部分内容可能为虚构，建议通过学术数据库验证)：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant Human ZNF132 Protein in Cancer Cell Lines*
**作者**: Zhang L, et al.
**摘要**: 本研究利用哺乳动物表达系统成功表达并纯化了重组人ZNF132蛋白，并发现其在体外抑制结肠癌细胞增殖。实验表明ZNF132可能通过调控Wnt/β-catenin信号通路发挥抑癌作用。
2. **文献名称**: *Structural Characterization of the Zinc Finger Domains in Human ZNF132 Protein*
**作者**: Smith J, et al.
**摘要**: 通过X射线晶体学解析了ZNF132的锌指结构域三维结构，揭示了其与特定DNA序列结合的分子机制。重组蛋白的纯化为结构分析提供了高纯度样品。
3. **文献名称**: *ZNF132 Promotes Neuronal Differentiation via Epigenetic Regulation*
**作者**: Tanaka K, et al.
**摘要**: 发现重组ZNF132蛋白可结合神经分化相关基因启动子区域，并通过招募组蛋白修饰酶HDAC1调控表观遗传状态，诱导小鼠神经干细胞分化。
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**注**：以上文献为示例性内容，实际研究中ZNF132相关文献可能较少，建议结合“ZNF132”“重组蛋白”“锌指蛋白”等关键词在PubMed、Google Scholar等平台检索最新成果。部分研究可能侧重ZNF家族其他成员(如ZNF143、ZNF148等)，需注意区分。
Zinc finger protein 132 (ZNF132) is a member of the zinc finger protein family, characterized by conserved C2H2-type zinc finger domains that mediate sequence-specific DNA binding and participate in transcriptional regulation. As a Krüppel-associated box (KRAB)-containing zinc finger protein, ZNF132 is speculated to function in chromatin remodeling and epigenetic gene silencing by recruiting co-repressor complexes. The human ZNF132 gene, located on chromosome 19. encodes a protein predominantly expressed in epithelial tissues, though its precise physiological roles remain understudied.
Recombinant human ZNF132 protein is engineered using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to enable functional studies, as low endogenous expression and technical limitations hinder isolation of native protein. This recombinant tool facilitates research into ZNF132's potential involvement in cellular processes like differentiation, proliferation, and apoptosis. Emerging evidence links dysregulated ZNF132 expression to cancers, including gastric and colorectal cancers, where it may act as a tumor suppressor through promoter hypermethylation or interaction with oncogenic pathways. However, mechanistic insights remain sparse, prompting ongoing investigations into its regulatory networks and disease associations. The protein’s structure-function relationships, particularly how its zinc finger motifs mediate target gene recognition, are key research focuses to decipher its biological and pathological relevance.
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