| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF148 |
| Uniprot No | Q9UQR1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-794 aa |
| 活性数据 | MNIDDKLEGL FLKCGGIDEM QSSRTMVVMG GVSGQSTVSG ELQDSVLQDR SMPHQEILAA DEVLQESEMR QQDMISHDEL MVHEETVKND EEQMETHERL PQGLQYALNV PISVKQEITF TDVSEQLMRD KKQIREPVDL QKKKKRKQRS PAKILTINED GSLGLKTPKS HVCEHCNAAF RTNYHLQRHV FIHTGEKPFQ CSQCDMRFIQ KYLLQRHEKI HTGEKPFRCD ECGMRFIQKY HMERHKRTHS GEKPYQCEYC LQYFSRTDRV LKHKRMCHEN HDKKLNRCAI KGGLLTSEED SGFSTSPKDN SLPKKKRQKT EKKSSGMDKE SALDKSDLKK DKNDYLPLYS SSTKVKDEYM VAEYAVEMPH SSVGGSHLED ASGEIHPPKL VLKKINSKRS LKQPLEQNQT ISPLSTYEES KVSKYAFELV DKQALLDSEG NADIDQVDNL QEGPSKPVHS STNYDDAMQF LKKKRYLQAA SNNSREYALN VGTIASQPSV TQAAVASVID ESTTASILES QALNVEIKSN HDKNVIPDEV LQTLLDHYSH KANGQHEISF SVADTEVTSS ISINSSEVPE VTPSENVGSS SQASSSDKAN MLQEYSKFLQ QALDRTSQND AYLNSPSLNF VTDNQTLPNQ PAFSSIDKQV YATMPINSFR SGMNSPLRTT PDKSHFGLIV GDSQHSFPFS GDETNHASAT STQDFLDQVT SQKKAEAQPV HQAYQMSSFE QPFRAPYHGS RAGIATQFST ANGQVNLRGP GTSAEFSEFP LVNVNDNRAG MTSSPDATTG QTFG |
| 分子量 | 88.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF148蛋白的3篇代表性文献摘要：
1. **"ZNF148 interacts with p53 and enhances its DNA binding and transcriptional activation in tumor suppression"**
*作者：Smith, J. et al. (2019)*
摘要：研究发现ZNF148通过直接结合p53蛋白，增强其DNA结合能力，促进肿瘤抑制相关基因的转录，揭示了ZNF148-p53互作在癌细胞凋亡调控中的关键作用。
2. **"Expression and purification of recombinant human ZNF148 for structural analysis"**
*作者：Chen, L. et al. (2020)*
摘要：成功在大肠杆菌系统中表达并纯化重组人ZNF148蛋白，通过X射线晶体学解析其锌指结构域的三维结构，为研究其DNA结合机制提供基础。
3. **"ZNF148 promotes TGF-β signaling and regulates metastasis in colorectal cancer"**
*作者：Wang, Y. et al. (2021)*
摘要：发现ZNF148通过激活TGF-β通路促进结直肠癌细胞上皮间质转化(EMT)，重组蛋白实验证实其直接调控靶基因SNAI1表达，提示其作为癌症治疗新靶点潜力。
注：以上文献为假设性示例，实际文献需通过PubMed等学术数据库检索获取最新研究。
Zinc finger protein 148 (ZNF148), also known as ZBP-89 or BERF-1. is a member of the Krüppel-type zinc finger transcription factor family. It binds to GC-rich DNA sequences through its conserved zinc finger domains, regulating the expression of target genes involved in cell proliferation, differentiation, and apoptosis. ZNF148 interacts with chromatin-modifying complexes and other transcription regulators, such as histone deacetylases (HDACs) or β-catenin, to modulate transcriptional activity. Studies suggest its dual roles in tumorigenesis, acting as a tumor suppressor or oncogene depending on cellular context. For example, it induces cell cycle arrest by activating p21 in colorectal cancer but promotes growth in gastric cancer through cooperation with β-catenin.
Recombinant human ZNF148 protein is typically produced in Escherichia coli or mammalian expression systems, often fused with tags (e.g., His-tag) for purification. It enables in vitro studies of DNA-protein interactions, structural analyses, and functional assays to dissect its molecular mechanisms. Researchers also utilize it to explore therapeutic strategies targeting ZNF148-associated pathways in diseases like cancer, fibrosis, and neurodegenerative disorders. Its modular structure, including the N-terminal KRAB domain and C-terminal DNA-binding zinc fingers, makes it a focus for understanding gene regulation networks.
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