| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF205 |
| Uniprot No | O95201 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-554 aa |
| 活性数据 | MSADGGGIQD TQDKETPPEV PDRGHPHQEM PSKLGEAVPS GDTQESLHIK MEPEEPHSEG ASQEDGAQGA WGWAPLSHGS KEKALFLPGG ALPSPRIPVL SREGRTRDRQ MAAALLTAWS QMPVTFEDVA LYLSREEWGR LDHTQQNFYR DVLQKKNGLS LGFPFSRPFW APQAHGKGEA SGSSRQAGDE KEWRGACTGA VEVGQRVQTS SVAALGNVKP FRTRAGRVQW GVPQCAQEAA CGRSSGPAKD SGQPAEPDRT PDAAPPDPSP TEPQEYRVPE KPNEEEKGAP ESGEEGLAPD SEVGRKSYRC EQCGKGFSWH SHLVTHRRTH TGEKPYACTD CGKRFGRSSH LIQHQIIHTG EKPYTCPACR KSFSHHSTLI QHQRIHTGEK PYVCDRCAKR FTRRSDLVTH QGTHTGAKPH KCPICAKCFT QSSALVTHQR THTGVKPYPC PECGKCFSQR SNLIAHNRTH TGEKPYHCLD CGKSFSHSSH LTAHQRTHRG VRPYACPLCG KSFSRRSNLH RHEKIHTTGP KALAMLMLGA AAAGALATPP PAPT |
| 分子量 | 60.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇模拟生成的重组人ZNF205蛋白相关参考文献示例(注意：实际文献需通过PubMed/Google Scholar等验证)：
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1. **文献名称**：ZNF205在肺癌细胞增殖中的调控作用及重组蛋白制备
**作者**：Zhang L., et al.
**摘要**：本研究成功通过大肠杆菌系统表达了重组人ZNF205蛋白，证明其通过结合特定DNA序列抑制肺癌细胞周期进展，揭示了其作为肿瘤抑制因子的潜力。
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2. **文献名称**：ZNF205锌指结构域与基因组靶点相互作用的体外分析
**作者**：Wang Y., et al.
**摘要**：利用重组ZNF205蛋白进行体外EMSA实验，确定了其锌指结构域优先识别"GCCCT"基序，为解析其在胚胎发育中的转录调控机制提供依据。
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3. **文献名称**：CRISPR/Cas9介导的ZNF205敲除对神经干细胞分化的影响
**作者**：Chen X., et al.
**摘要**：结合重组ZNF205蛋白回补实验，证实该蛋白通过调控Notch信号通路促进神经干细胞向神经元分化，提示其在中枢神经系统疾病中的潜在应用价值。
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**重要提醒**：
以上文献为模拟示例，实际研究请通过权威数据库检索(如PMID编号)。建议使用关键词“ZNF205”或“zinc finger protein 205 recombinant human”在PubMed中获取真实文献信息。部分相关研究可能被归类在锌指蛋白家族(例如KRAB-ZFP亚族)的功能分析中。
Zinc finger protein 205 (ZNF205), a member of the zinc finger protein family, is characterized by its conserved C2H2-type zinc finger motifs, which enable sequence-specific DNA or RNA binding. Predominantly expressed in human tissues, ZNF205 is hypothesized to function as a transcription regulator, influencing gene expression programs involved in cellular differentiation, proliferation, or apoptosis. Its structural organization includes multiple tandem zinc finger repeats, typically associated with nucleic acid interaction and protein-DNA complex formation.
Recombinant human ZNF205 protein is produced via molecular cloning, often in prokaryotic (e.g., *E. coli*) or eukaryotic expression systems, ensuring proper folding and post-translational modifications for functional studies. This engineered protein serves as a critical tool to unravel ZNF205's biological roles, such as its potential involvement in embryogenesis, tissue development, and disease pathways. Emerging research links ZNF205 dysregulation to cancers and genetic disorders, though mechanistic insights remain limited. Current applications include in vitro binding assays, chromatin immunoprecipitation, and cellular localization studies to map its regulatory targets. Further investigations aim to explore its therapeutic potential, diagnostic relevance, or utility in synthetic biology frameworks. The recombinant form accelerates research by providing a purified, standardized reagent for structural and functional characterization.
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