| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF18 |
| Uniprot No | P17022 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 316-586 aa |
| 活性数据 | QRTHLVQHQRIHTGEKPYTCNECGKAFSQRGHFMEHQKIHTGEKPFKCDECDKTFTRSTHLTQHQKIHTGEKTYKCNECGKAFNGPSTFIRHHMIHTGEKPYECNECGKAFSQHSNLTQHQKTHTGEKPYDCAECGKSFSYWSSLAQHLKIHTGEKPYKCNECGKAFSYCSSLTQHRRIHTREKPFECSECGKAFSYLSNLNQHQKTHTQEKAYECKECGKAFIRSSSLAKHERIHTGEKPYQCHECGKTFSYGSSLIQHRKIHTGERPY |
| 分子量 | 31 kDa |
| 蛋白标签 | His-DHFR tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为虚构示例(因实际文献可能较少，建议通过学术数据库验证)：
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1. **"Cloning and Expression Analysis of Human ZNF18 Protein in Prokaryotic Systems"**
*Zhang, L. et al.*
研究报道了ZNF18基因的原核表达系统构建及重组蛋白纯化，证明其具有结合特定DNA序列的活性，为功能研究奠定基础。
2. **"ZNF18 as a Potential Regulator in Stem Cell Differentiation"**
*Kim, S. & Patel, R.*
摘要提出ZNF18通过调控靶基因转录影响胚胎干细胞分化，其重组蛋白的过表达实验显示与细胞周期调控相关通路有关。
3. **"Structural Characterization of Recombinant ZNF18 Zinc Finger Domains"**
*Gómez, M.T. et al.*
该文献通过X射线晶体学解析ZNF18锌指结构域的三维构象，揭示其与DNA结合的关键氨基酸残基及潜在功能位点。
4. **"ZNF18 Interactions with Chromatin Remodeling Complexes"**
*Chen, X. et al.*
研究表明重组ZNF18蛋白在体外与组蛋白修饰酶复合物结合，暗示其在表观遗传调控中可能起到桥梁作用。
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注：上述内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索确认。
ZNF18 (Zinc Finger Protein 18), also known as HTF10 or ZNF157. is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KRAB-ZFP) family. These proteins are characterized by tandem arrays of C2H2-type zinc finger motifs, which mediate sequence-specific DNA binding, and an N-terminal KRAB domain that often functions as a transcriptional repression module by recruiting chromatin-modifying complexes. ZNF18 is encoded by a human gene located on chromosome 16q22.1 and is believed to play roles in gene regulation, cellular differentiation, and proliferation. Its expression has been detected in various tissues, with notable activity in hematopoietic and immune systems.
Recombinant human ZNF18 protein is artificially expressed in laboratory systems (e.g., E. coli, mammalian cells) for functional studies. Research suggests its involvement in chromatin remodeling, regulation of target gene networks, and potential links to hematological malignancies or autoimmune disorders. Structural studies focus on its zinc finger domains' DNA-binding specificity and KRAB-mediated protein interactions. Current applications include investigating its role in transcriptional regulation pathways and developing potential diagnostic or therapeutic strategies for ZNF18-associated diseases. Ongoing research aims to clarify its precise physiological targets and pathological mechanisms in human cancers and immune dysregulation.
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