| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF185 |
| Uniprot No | O15231 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-689 aa |
| 活性数据 | MSISALGGRT KGKPLPPGEE ERNNVLKQMK VRTTLKGDKS WITKQDESEG RTIELPSGRS RATSFSSAGE VPKPRPPSTR APTGYIIRGV FTKPIDSSSQ PQQQFPKANG TPKSAASLVR TANAGPPRPS SSGYKMTTED YKKLAPYNIR RSSTSGDTEE EEEEEVVPFS SDEQKRRSEA ASGVLRRTAP REHSYVLSAA KKSTGPTQET QAPFIAKRVE VVEEDGPSEK SQDPPALARS TPGSNSADGG RTKASRAIWI ECLPSMPSPA GSQELSSRGE EIVRLQILTP RAGLRLVAPD VEGMRSSPGN KDKEAPCSRE LQRDLAGEEA FRAPNTDAAR SSAQLSDGNV GSGATGSRPE GLAAVDIGSE RGSSSATSVS AVPADRKSNS TAAQEDAKAD PKGALADYEG KDVATRVGEA WQERPGAPRG GQGDPAVPAQ QPADPSTPER QSSPSGSEQL VRRESCGSSV LTDFEGKDVA TKVGEAWQDR PGAPRGGQGD PAVPTQQPAD PSTPEQQNSP SGSEQFVRRE SCTSRVRSPS SCMVTVTVTA TSEQPHIYIP APASELDSSS TTKGILFVKE YVNASEVSSG KPVSARYSNV SSIEDSFAME KKPPCGSTPY SERTTGGICT YCNREIRDCP KITLEHLGIC CHEYCFKCGI CSKPMGDLLD QIFIHRDTIH CGKCYEKLF |
| 分子量 | 73.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF185蛋白的参考文献示例(注：内容为虚构示例，如需真实文献请通过学术数据库检索)：
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1. **文献名称**: *ZNF185 suppresses prostate cancer progression by inhibiting cell migration through regulation of EMT-related markers*
**作者**: Chen L, et al.
**摘要**: 本研究揭示了ZNF185在前列腺癌中的抑癌功能。通过体外实验发现，过表达ZNF185可显著抑制肿瘤细胞的迁移和侵袭，并下调上皮-间质转化(EMT)标志物(如Snail和Vimentin)。机制分析表明，ZNF185可能通过整合素信号通路调控细胞粘附。
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2. **文献名称**: *The role of ZNF185 in ovarian cancer: Impacts on apoptosis and chemoresistance*
**作者**: Wang Y, et al.
**摘要**: 该研究探讨了ZNF185在卵巢癌中的表达与化疗敏感性的关系。实验表明，ZNF185低表达与患者不良预后相关。RNA干扰敲低ZNF185后，癌细胞对顺铂的耐药性增强，凋亡减少，提示ZNF185可能通过调控BCL-2家族蛋白影响线粒体凋亡途径。
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3. **文献名称**: *ZNF185 interacts with cytoskeletal proteins and modulates hepatocellular carcinoma metastasis*
**作者**: Gupta R, et al.
**摘要**: 本研究通过免疫共沉淀技术发现ZNF185与α-actinin等细胞骨架蛋白直接互作。在肝癌模型中，ZNF185的缺失导致细胞运动能力增强，可能通过破坏细胞骨架稳定性促进转移。动物实验进一步证实了ZNF185对肝内转移的抑制作用。
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4. **文献名称**: *Epigenetic silencing of ZNF185 in breast cancer and its functional recovery by recombinant protein delivery*
**作者**: Kim S, et al.
**摘要**: 文章报道了重组人ZNF185蛋白在乳腺癌细胞中的功能恢复实验。DNA甲基化导致ZNF185在乳腺癌中表达沉默，而外源性重组ZNF185蛋白处理可显著抑制细胞增殖并诱导G1期阻滞，为基于ZNF185的蛋白疗法提供了理论依据。
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如需真实文献，建议通过PubMed或Web of Science使用关键词“ZNF185”“cancer”“recombinant protein”等检索。
Zinc finger protein 185 (ZNF185), a member of the LIM domain-containing zinc finger protein family, is encoded by the ZNF185 gene located on human chromosome Xq28. Characterized by its unique LIM domain—a double zinc finger motif—ZNF185 is implicated in cytoskeletal organization, cell adhesion, and signaling pathways. It is highly expressed in tissues like the testes, prostate, and certain epithelial cells, suggesting roles in cellular differentiation and tissue homeostasis. Studies highlight its interaction with integrins and other cell surface receptors, facilitating communication between the extracellular matrix and intracellular signaling cascades. Dysregulation of ZNF185 has been linked to cancer progression, particularly in prostate and bladder cancers, where its downregulation correlates with tumor invasiveness and poor prognosis. As a tumor suppressor candidate, it may inhibit metastasis by modulating cell motility and cytoskeletal dynamics. Recombinant ZNF185 protein, typically produced via bacterial or mammalian expression systems, is utilized to study its molecular functions, including DNA damage responses and stress signaling. Its recombinant form also holds potential for therapeutic exploration, such as targeting cancer pathways or restoring cellular adhesion defects. Ongoing research aims to clarify its precise mechanisms and clinical relevance in disease contexts.
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