| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF17 |
| Uniprot No | P17021 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-662 aa |
| 活性数据 | MNLTEDYMVF EDVAIHFSQE EWGILNDVQR HLHSDVMLEN FALLSSVGCW HGAKDEEAPS KQCVSVGVSQ VTTLKPALST QKAQPCETCS SLLKDILHLA EHDGTHPKRT AKLYLHQKEH LREKLTRSDE GRPSFVNDSV HLAKRNLTCM QGGKDFTGDS DLQQQALHSG WKPHRDTHGV EAFQSGQNNY SCTQCGKDFC HQHTLFEHQK IHTEERPYEC SECGKLFRYN SDLIKHQRNH TGERPYKCSE CGKAFSLKYN VVQHQKIHTG ERPYECSECG KAFLRKSHLL QHQRIHTRPR PYVCSECGKA FLTQAHLVGH QKIHTGERPY GCNECGKYFM YSSALIRHQK VHTGERPFYC CECGKFFMDS CTLIIHQRVH TGEKPYECNE CGKFFRYRST LIRHQKVHTG EKPYECSECG KFFMDTSTLI IHQRVHTGEK PYECNKCGKF FRYCFTLNRH QRVHSGERPY ECSECGKFFV DSCTLKSHQR VHTGERPFEC SICGKSFRCR STLDTHQRIH TGERPYECSE CGKFFRHNSN HIRHRRNHFG ERSFECTECG RVFSQNSHLI RHQKVHTRER TYKCSKCGKF FMDSSTLISH ERVHTGEKPY ECSECGKVFR YNSSLIKHRR IHTGERPYQC SECGRVFNQN SHLIQHQKVH TR |
| 分子量 | 77.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF17蛋白的模拟参考文献示例(注：以下内容为基于学术常识的示例，实际文献需通过PubMed、Google Scholar等平台查询)：
### 1. **"Cloning and Functional Characterization of Recombinant Human ZNF17 Protein"**
*作者：Lee, S. et al.*
**摘要**：研究报道了ZNF17的克隆及在大肠杆菌中的重组表达，通过质谱和凝胶迁移实验确认其DNA结合活性，并发现其可能通过KRAB结构域招募转录抑制复合物调控靶基因表达。
### 2. **"Structural Insights into the Zinc Finger Domains of ZNF17"**
*作者：Zhang, Y. & Wang, M.*
**摘要**：利用X射线晶体学解析ZNF17的锌指结构域，揭示其特异性DNA结合模体，并探讨其与染色质重塑蛋白的相互作用机制。
### 3. **"ZNF17 Modulates Cell Proliferation in Colorectal Cancer via Epigenetic Regulation"**
*作者：Chen, H. et al.*
**摘要**：研究发现重组ZNF17在结直肠癌细胞中过表达可抑制细胞增殖，机制涉及靶基因启动子的甲基化修饰及组蛋白去乙酰化酶(HDAC)的募集。
### 4. **"Expression Optimization of Recombinant ZNF17 in Mammalian Cells for Functional Studies"**
*作者：Gupta, R. & Patel, K.*
**摘要**：优化了ZNF17在HEK293细胞中的重组表达条件，证实其核定位特性，并通过ChIP-seq鉴定其在基因组中的结合位点，提示其在发育调控中的作用。
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建议通过学术数据库输入关键词 **"ZNF17"、"recombinant ZNF17"、"zinc finger protein 17 function"** 获取真实文献。
Recombinant human ZNF17 protein is a synthetically produced version of the zinc finger protein 17. encoded by the *ZNF17* gene in humans. As a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZFP) family, ZNF17 features a conserved N-terminal KRAB domain and multiple C-terminal C2H2-type zinc finger motifs. The KRAB domain mediates protein-protein interactions, often recruiting chromatin-modifying complexes to repress transcription, while the zinc finger domains enable sequence-specific DNA binding. ZNF17 is implicated in transcriptional regulation, particularly in gene silencing via epigenetic mechanisms, such as histone deacetylation or methylation. Studies suggest its involvement in developmental processes, cellular differentiation, and maintenance of stem cell pluripotency. Dysregulation of ZNF17 has been observed in certain cancers, though its role remains controversial—it may act as a tumor suppressor or oncogene depending on cellular context. For instance, aberrant expression correlates with prostate cancer progression and neural malignancies. Recombinant ZNF17 is typically generated using bacterial or mammalian expression systems for in vitro studies, enabling mechanistic exploration of its DNA-binding specificity, interaction partners, and regulatory networks. Despite progress, its precise biological functions and disease relevance require further elucidation, highlighting the importance of ongoing structural and functional research.
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