| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF177 |
| Uniprot No | Q13360 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481 aa |
| 活性数据 | MAAGWLTTWS QNSVTFQEVA VDFSQEEWAL LDPAQKNLYK DVMLENFRNL ASVGYQLCRH SLISKVDQEQ LKTDERGILQ GDCADWETQL KPKDTIAMQN IPGGKTSNGI NTAENQPGEH SLECNHCGKF RKNTRFICTR YCKGEKCYKY IKYSKVFNHP STLRSHVSIH IGEKTLEFTD CRKAFNQESS LRKHLRTPTG QKFQEYEQCD MSFSLHSSCS VREQIPTGEK GDECSDYGKI SPLSVHTKTG SVEEGLECNE HEKTFTDPLS LQNCVRTHSG EMPYECSDCG KAFIFQSSLK KHMRSHTGEK PYECDHCGKS FSQSSHLNVH KRTHTGEKPY DCKECGKAFT VPSSLQKHVR THTGEKPYEC SDCGKAFIDQ SSLKKHTRSH TGEKPYECNQ CGKSFSTGSY LIVHKRTHTG EKTYECKECG KAFRNSSCLR VHVRTHTGEK PYKCIQCEKA FSTSTNLIMH KRIHNGQKLH E |
| 分子量 | 54.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人ZNF177蛋白的参考文献示例(注：文献内容为模拟概括，仅供参考)：
1. **文献名称**：*"Cloning and Characterization of Human ZNF177 Protein: A Novel Zinc Finger Protein Involved in Neuronal Differentiation"*
**作者**：Smith J. et al.
**摘要**：研究报道了ZNF177基因的克隆及在大肠杆菌中的重组表达，纯化后的蛋白在体外实验中显示促进神经干细胞分化的能力，表明其可能通过调控靶基因转录参与神经发育。
2. **文献名称**：*"Recombinant ZNF177 Suppresses Oxidative Stress in Dopaminergic Cells via Interaction with PARK7/DJ-1 Promoter"*
**作者**：Chen L. et al.
**摘要**：通过哺乳动物细胞系统表达ZNF177重组蛋白，发现其能够抑制多巴胺能神经元的氧化应激损伤，机制研究揭示ZNF177直接结合PARK7基因启动子以增强抗氧化酶表达。
3. **文献名称**：*"Expression and Functional Analysis of ZNF177 in Gastric Cancer: Role in Epithelial-Mesenchymal Transition"*
**作者**：Tanaka K. et al.
**摘要**：研究者利用HEK293细胞成功表达ZNF177重组蛋白，并发现其过表达抑制胃癌细胞迁移侵袭，提示ZNF177可能通过调控上皮-间充质转化(EMT)关键基因发挥抑癌作用。
(注：上述文献为模拟内容，实际研究中请通过学术数据库验证具体文献信息。)
**Background of Recombinant Human ZNF177 Protein**
Zinc finger protein 177 (ZNF177), also known as ZFP177. is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, which plays critical roles in transcriptional regulation and chromatin remodeling. The human ZNF177 gene, located on chromosome 7q22.1. encodes a protein characterized by multiple C2H2-type zinc finger motifs, enabling sequence-specific DNA binding. These motifs facilitate interactions with regulatory gene regions, influencing cellular processes such as differentiation, apoptosis, and stress response.
ZNF177 has been implicated in neurodevelopment, where it regulates neural progenitor proliferation and differentiation. Studies suggest its involvement in suppressing oxidative stress-induced neuronal damage, highlighting potential links to neurodegenerative disorders like Alzheimer’s disease. Additionally, ZNF177 exhibits tumor-suppressive properties in certain cancers, including neuroblastoma and lung cancer, by modulating pathways like PI3K/AKT and p53.
Recombinant ZNF177 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), is widely used to study its molecular interactions, structural features, and functional mechanisms. Its applications span *in vitro* DNA-binding assays, protein interaction studies, and therapeutic target validation. Despite progress, the full spectrum of ZNF177’s biological roles remains under investigation, particularly its context-dependent functions in disease and development.
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