| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF154 |
| Uniprot No | Q13106 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437 aa |
| 活性数据 | MAAATLRTPT QGTVTFEDVA VHFSWEEWGL LDEAQRCLYR DVMLENLALL TSLDVHHQKQ HLGEKHFRSN VGRALFVKTC TFHVSGEPST CREVGKDFLA KLGFLHQQAA HTGEQSNSKS DGGAISHRGK THYNCGEHTK AFSGKHTLVQ QQRTLTTERC YICSECGKSF SKSYSLNDHW RLHTGEKPYE CRECGKSFRQ SSSLIQHRRV HTAVRPHECD ECGKLFSNKS NLIKHRRVHT GERPYECSEC GKSFSQRSAL LQHRGVHTGE RPYECSECGK FFTYHSSLIK HQKVHSGSRP YECSECGKSF SQNSSLIEHH RVHTGERPYK CSECGKSFSQ RSALLQHRGV HTGERPYECS ECGKFFPYSS SLRKHQRVHT GSRPYECSEC GKSFTQNSGL IKHRRVHTGE KPYECTECGK SFSHNSSLIK HQRIHSR |
| 分子量 | 49.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF154蛋白的3篇模拟参考文献示例(基于研究领域常见方向，非真实文献)：
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1. **文献名称**: "Expression and purification of recombinant human ZNF154 in *E. coli* for structural studies"
**作者**: Zhang L, et al.
**摘要**: 研究报道了在大肠杆菌系统中表达重组人ZNF154蛋白的优化方法，通过His标签纯化获得高纯度蛋白，并利用圆二色谱分析其锌指结构域构象，为后续DNA结合功能研究提供基础。
2. **文献名称**: "ZNF154 promotes DNA methylation in human cancer cells via recruitment of DNMT3A"
**作者**: Wang Y, et al.
**摘要**: 研究发现重组ZNF154蛋白在体外增强DNA甲基转移酶DNMT3A的活性，通过染色质免疫共沉淀(ChIP)验证其在癌细胞中靶向特定基因启动子区域并促进异常甲基化，提示其在表观遗传调控中的作用。
3. **文献名称**: "Functional characterization of ZNF154 mutations in genetic disorders"
**作者**: Gupta R, et al.
**摘要**: 通过体外表达突变型重组ZNF154蛋白，分析其对DNA结合能力的丧失及其与发育异常疾病的关联，揭示了ZNF154锌指结构域关键氨基酸的功能重要性。
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**说明**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台以“ZNF154 recombinant protein”“ZNF154 expression”等关键词检索真实文献。
Zinc finger protein 154 (ZNF154) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, characterized by tandem C2H2-type zinc finger motifs that mediate sequence-specific DNA binding. As a transcription factor, ZNF154 is postulated to regulate gene expression through epigenetic mechanisms, particularly by recruiting chromatin-modifying complexes to targeted genomic regions. It is ubiquitously expressed in human tissues and plays potential roles in cellular differentiation, development, and genome stability maintenance.
Recent studies highlight ZNF154's association with cancer biology. Hypermethylation of its promoter region has been observed in multiple cancers, including colorectal, ovarian, and cervical cancers, suggesting its utility as an epigenetic biomarker for early detection. Additionally, ZNF154 may function as a tumor suppressor, as its downregulation correlates with malignant progression and poor prognosis.
Recombinant human ZNF154 protein, typically produced in Escherichia coli or mammalian expression systems, retains DNA-binding activity and is widely used to investigate its molecular interactions, structural properties, and regulatory mechanisms. Researchers employ it in electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP), and functional studies to map DNA-binding sites and explore its role in gene silencing. Optimizing soluble expression and post-translational modifications (e.g., phosphorylation) remains critical for enhancing its stability and functional reproducibility in vitro.
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