| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF14 |
| Uniprot No | P17017 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-642 aa |
| 活性数据 | MDSVSFEDVA VNFTLEEWAL LDSSQKKLYE DVMQETFKNL VCLGKKWEDQ DIEDDHRNQG KNRRCHMVER LCESRRGSKC GETTSQMPNV NINKETFTGA KPHECSFCGR DFIHHSSLNR HMRSHTGQKP NEYQEYEKQP CKCKAVGKTF SYHHCFRKHE RTHTGVKPYE CKQCGKAFIY YQPFQRHERT HAGQKPYECK QCGKTFIYYQ SFQKHAHTGK KPYECKQCGK AFICYQSFQR HKRTHTGEKP YECKQCGKAF SCPTYFRTHE RTHTGEKPYK CKECGKAFSF LSSFRRHKRT HSGEKPYECK ECGKAFFYSA SFRAHVIIHT GARPYKCKEC GKAFNSSNSC RVHERTHIGE KPYECKRCGK SFSWSISLRL HERTHTGEKP YECKQCHKTF SFSSSLREHE TTHTGEKPYE CKQCGKTFSF SSSLQRHERT HNAEKPYECK QCGKAFRCSS YFRIHERSHT GEKPYECKQC GKVFIRSSSF RLHERTHTGE KPYECKLCGK TFSFSSSLRE HEKIHTGNKP FECKQCGKAF LRSSQIRLHE RTHTGEKPYQ CKQCGKAFIS SSKFRMHERT HTGEKPYRCK QCGKAFRFSS SVRIHERSHT GEKPYECKQC GKAFISSSHF RLHERTHMGE KV |
| 分子量 | 75.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF14蛋白的参考文献示例(注：ZNF14具体研究可能有限，以下内容为模拟示例)：
1. **"Expression and functional analysis of recombinant human ZNF14 in transcriptional regulation"**
- 作者：Lee, S. et al.
- 摘要：该研究通过大肠杆菌系统成功表达并纯化了重组人ZNF14蛋白，证实其在体外能够结合特定DNA序列，并通过荧光素酶报告基因实验揭示其抑制靶基因转录的活性。
2. **"Structural characterization of the zinc finger domain of ZNF14 and implications for protein-DNA interactions"**
- 作者：Martínez, R. & García, A.
- 摘要：利用X射线晶体学解析了重组ZNF14蛋白锌指结构域的三维结构，揭示了其与DNA结合的分子机制，并发现特定氨基酸残基对结合亲和力至关重要。
3. **"Role of ZNF14 in cellular differentiation: Insights from recombinant protein overexpression"**
- 作者：Chen, X. et al.
- 摘要：在HEK293细胞中过表达重组ZNF14蛋白后，观察到其通过调控Wnt信号通路相关基因表达，抑制细胞增殖并促进成骨分化。
4. **"High-yield production of recombinant human ZNF14 in insect cells and functional screening"**
- 作者：Wang, L. & Ito, T.
- 摘要：采用杆状病毒-昆虫细胞系统高效表达ZNF14蛋白，优化纯化工艺后验证了其在体外的核酸酶抗性及对染色质重塑复合物的潜在调控作用。
(注：以上文献及作者为虚拟示例，实际研究中请根据具体内容检索核实。)
**Background of Recombinant Human ZNF14 Protein**
Zinc finger protein 14 (ZNF14), a member of the Krüppel-associated box (KRAB) zinc finger protein (ZFP) family, is a transcription factor involved in gene regulation. These proteins are characterized by tandem arrays of Cys2-His2 zinc finger motifs that bind DNA, and a KRAB domain at the N-terminus, which mediates interactions with co-repressors to regulate transcriptional silencing. ZNF14. encoded by the *ZNF14* gene on human chromosome 19. is thought to play roles in cellular differentiation, development, and genome stability, particularly through repressing retrotransposons or modulating chromatin structure.
Recombinant ZNF14 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. It enables studies on DNA-binding specificity, protein-protein interactions, and mechanisms of transcriptional repression. Researchers utilize recombinant ZNF14 to explore its potential roles in diseases, including cancers or genetic disorders linked to dysregulated zinc finger proteins. Its structural and functional analysis also contributes to developing targeted therapies or synthetic transcriptional regulators.
Current research focuses on elucidating ZNF14’s target genes, interaction partners, and physiological impact, leveraging techniques like ChIP-seq, CRISPR, and *in vitro* binding assays. Despite limited direct data on ZNF14. its conserved domains suggest functional overlap with other KRAB-ZFPs, emphasizing its importance in epigenetic regulation and genome defense.
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