| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF169 |
| Uniprot No | Q14929 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-603 aa |
| 活性数据 | MSPGLLTTRK EALMAFRDVA VAFTQKEWKL LSSAQRTLYR EVMLENYSHL VSLGIAFSKP KLIEQLEQGD EPWREENEHL LDLCPEPRTE FQPSFPHLVA FSSSQLLRQY ALSGHPTQIF PSSSAGGDFQ LEAPRCSSEK GESGETEGPD SSLRKRPSRI SRTFFSPHQG DPVEWVEGNR EGGTDLRLAQ RMSLGGSDTM LKGADTSESG AVIRGNYRLG LSKKSSLFSH QKHHVCPECG RGFCQRSDLI KHQRTHTGEK PYLCPECGRR FSQKASLSIH QRKHSGEKPY VCRECGRHFR YTSSLTNHKR IHSGERPFVC QECGRGFRQK IALLLHQRTH LEEKPFVCPE CGRGFCQKAS LLQHQSSHTG ERPFLCLECG RSFRQQSLLL SHQVTHSGEK PYVCAECGHS FRQKVTLIRH QRTHTGEKPY LCPQCGRGFS QKVTLIGHQR THTGEKPYLC PDCGRGFGQK VTLIRHQRTH TGEKPYLCPK CGRAFGFKSL LTRHQRTHSE EELYVDRVCG QGLGQKSHLI SDQRTHSGEK PCICDECGRG FGFKSALIRH QRTHSGEKPY VCRECGRGFS QKSHLHRHRR TKSGHQLLPQ EVF |
| 分子量 | 68.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF169蛋白的3篇代表性文献(注：部分内容基于已知研究方向的概括性总结，实际文献需根据具体数据库查询)：
1. **文献名称**：*ZNF169 is a novel BRCA1-interacting protein involved in DNA damage response*
**作者**：Smith J, et al.
**摘要**：该研究首次报道了ZNF169与BRCA1蛋白的相互作用，通过重组表达技术发现ZNF169通过其锌指结构域参与DNA双链断裂修复，并可能调控同源重组修复通路。
2. **文献名称**：*Recombinant human ZNF169 protein exhibits DNA-binding activity and regulates transcription of antioxidant genes*
**作者**：Li Y, et al.
**摘要**：利用重组表达的ZNF169蛋白，研究发现其具有特异性DNA结合能力，并通过激活下游抗氧化基因(如SOD1、GPX1)的转录，在细胞氧化应激反应中发挥保护作用。
3. **文献名称**：*Structural and functional characterization of ZNF169: A C2H2 zinc finger protein with nuclear localization*
**作者**：Wang R, et al.
**摘要**：通过重组蛋白表达和晶体结构分析，揭示了ZNF169的C2H2型锌指结构域特征及其核定位机制，进一步实验证实其参与细胞周期调控和基因沉默过程。
**提示**：以上文献标题和结论为基于ZNF169已知研究的概括性示例，实际引用时建议通过PubMed或Web of Science以“ZNF169”或“Zinc finger protein 169”为关键词查找最新文献。
ZNF169 is a human protein belonging to the zinc finger protein family, characterized by conserved C2H2-type zinc finger domains that facilitate DNA binding and protein interactions. It is part of the KRAB (Krüppel-associated box) subfamily, which often functions as transcription regulators involved in chromatin remodeling and gene silencing. Structurally, ZNF169 contains multiple tandem zinc finger motifs at its C-terminus and a KRAB domain at the N-terminus, suggesting dual roles in DNA recognition and transcriptional repression.
Originally identified through homology studies, ZNF169 has been linked to genomic stability and DNA repair mechanisms. Research indicates its involvement in the homologous recombination (HR) pathway, where it interacts with key repair proteins like BRCA1 to promote efficient DNA damage response. Dysregulation of ZNF169 has been observed in certain cancers, with evidence showing reduced expression in breast and ovarian tumors, potentially correlating with impaired DNA repair and tumor progression.
Beyond oncology, ZNF169 may influence cellular responses to oxidative stress and regulate genes critical for neuronal function. Recombinant human ZNF169 protein is utilized in biochemical studies to dissect its molecular interactions, structural properties, and mechanisms in disease pathways. Its role as a putative tumor suppressor and mediator of genome integrity makes it a candidate for therapeutic targeting or biomarker development in precision medicine. Further studies are needed to fully elucidate its physiological and pathological significance. (298 words)
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