| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF174 |
| Uniprot No | Q15697 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-407 aa |
| 活性数据 | MAAKMEITLS SNTEASSKQE RHIIAKLEEK RGPPLQKNCP DPELCRQSFR RFCYQEVSGP QEALSQLRQL CRQWLQPELH TKEQILELLV MEQFLTILPP EIQARVRHRC PMSSKEIVTL VEDFHRASKK PKQWVAVCMQ GQKVLLEKTG SQLGEQELPD FQPQTPRRDL RESSPAEPSQ AGAYDRLSPH HWEKSPLLQE PTPKLAGTEA PRMRSDNKEN PQQEGAKGAK PCAVSAGRSK GNGLQNPEPR GANMSEPRLS RRQVSSPNAQ KPFAHYQRHC RVEYISSPLK SHPLRELKKS KGGKRSLSNR LQHLGHQPTR SAKKPYKCDD CGKSFTWNSE LKRHKRVHTG ERPYTCGECG NCFGRQSTLK LHQRIHTGEK PYQCGQCGKS FRQSSNLHQH HRLHHGD |
| 分子量 | 46.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF174蛋白的示例性参考文献(文献信息为假设性描述，若需真实文献请告知进一步检索)：
1. **文献名称**：*ZNF174 regulates apoptosis via transcriptional control of pro-apoptotic genes*
**作者**：Smith A, et al.
**摘要**：研究揭示了ZNF174通过结合特定DNA序列调控细胞凋亡相关基因(如BAX、CASP3)的转录，其在肿瘤细胞中的异常表达与化疗耐药性相关。
2. **文献名称**：*Structural analysis of recombinant human ZNF174 and its DNA-binding specificity*
**作者**：Chen L, et al.
**摘要**：报道了重组人ZNF174蛋白的晶体结构，阐明其锌指结构域与靶DNA结合的特异性模式，并验证其在表观遗传调控中的潜在作用。
3. **文献名称**：*ZNF174 interacts with HDAC1 to repress gene expression in neuronal differentiation*
**作者**：Wang Y, et al.
**摘要**：证明ZNF174与组蛋白去乙酰化酶HDAC1形成复合物，抑制神经分化相关基因的表达，提示其在神经发育中的调控功能。
4. **文献名称**：*Downregulation of ZNF174 in colorectal cancer and its tumor-suppressive role*
**作者**：Kim H, et al.
**摘要**：发现结直肠癌中ZNF174表达显著降低，通过体外实验证实其重组蛋白过表达可抑制肿瘤细胞增殖并诱导周期阻滞。
**备注**：上述文献为示例，实际研究中ZNF174相关论文较少，建议通过PubMed或Web of Science以“ZNF174”或“Zinc finger protein 174”为关键词检索最新进展。
ZNF174 (Zinc Finger Protein 174) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, which represents one of the largest groups of transcription regulators in mammals. This protein is characterized by multiple C2H2-type zinc finger motifs at its C-terminal region, enabling sequence-specific DNA binding, and an N-terminal KRAB domain that typically mediates transcriptional repression by recruiting chromatin-modifying complexes like histone deacetylases (HDACs). ZNF174 is encoded by the human gene located on chromosome 16q22.1 and is implicated in gene regulation, though its precise biological functions remain under investigation.
Studies suggest ZNF174 may act as a transcriptional repressor, influencing cell proliferation, differentiation, and apoptosis. Intriguingly, it has been linked to cancer development with conflicting roles reported. Some research associates ZNF174 promoter hypermethylation with reduced expression in hepatocellular carcinoma and colorectal cancer, hinting at tumor-suppressive properties. Conversely, other studies indicate its oncogenic potential in breast cancer by promoting cell invasion. Recombinant human ZNF174 protein, produced via bacterial or eukaryotic expression systems, facilitates in vitro studies to dissect its DNA-binding specificity, interaction partners (e.g., TRIM28/KAP1), and regulatory mechanisms. Its involvement in epigenetic silencing pathways positions it as a potential biomarker or therapeutic target, though further functional validation in disease models is needed.
×
