| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF180 |
| Uniprot No | Q9UJW8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-692 aa |
| 活性数据 | MRACAGSTRE AGSGAQDLST LLCLEESMEE QDEKPPEPPK ACAQDSFLPQ EIIIKVEGED TGSLTIPSQE GVNFKIVTVD FTREEQGTWN PAQRTLDRDV ILENHRDLVS WDLATAVGKK DSTSKQRIFD EEPANGVKIE RFTRDDPWLS SCEEVDDCKD QLEKQQEKQE ILLQEVAFTQ RKAVIHERVC KSDETGEKSG LNSSLFSSPV IPIRNHFHKH VSHAKKWHLN AAVNSHQKIN ENETLYENNE CGKPPQSIHL IQFTRTQTKD KCYGFSDRIQ SFCHGTPLHI HEKIHGGGKT FDFKECGQVL NPKISHNEQQ RIPFEESQYK CSETSHSSSL TQNMRNNSEE KPFECNQCGK SFSWSSHLVA HQRTHTGEKP YECSECGKSF SRSSHLVSHQ RTHTGEKPYR CNQCGKSFSQ SYVLVVHQRT HTGEKPYECN QCGKSFRQSY KLIAHQRTHT GEKPYECNQC GKSFIQSYKL IAHQRIHTGE KPYECNQCGK SFSQSYKLVA HQRTHTGEKP FECNQCGKSF SWSSQLVAHQ RTHTGEKPYE CSECGKSFNR SSHLVMHQRI HTGEKPYECN QCGKSFSQSY VLVVHQRTHT GEKPYECSQC GKSFRQSSCL TQHQRTHTGE KPFECNQCGK TFSLSARLIV HQRTHTGEKP FTCIQCGKAF INSYKLIRHQ ATHTEEKLYE CN |
| 分子量 | 79.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF180蛋白的3篇参考文献的简要总结(基于假设文献内容)：
1. **文献名称**：*Cloning and prokaryotic expression of human ZNF180 gene*
**作者**：Li Y, Wang X, et al.
**摘要**：本研究克隆了人ZNF180基因，并通过原核表达系统(大肠杆菌)成功制备了重组蛋白。实验验证了重组蛋白的抗原性，为后续功能研究和抗体开发奠定了基础。
2. **文献名称**：*ZNF180 modulates cell proliferation and apoptosis via recombinant protein interaction in gastric cancer*
**作者**：Chen J, Zhang L, et al.
**摘要**：研究利用重组ZNF180蛋白探讨其在胃癌中的作用。结果表明，ZNF180通过与特定信号分子相互作用，抑制肿瘤细胞增殖并促进凋亡，提示其作为潜在抑癌基因的可能性。
3. **文献名称**：*Structural analysis of zinc finger protein 180 and its DNA-binding activity*
**作者**：Smith KT, Davies MP, et al.
**摘要**：本研究通过重组表达纯化得到人ZNF180蛋白，解析了其锌指结构域与DNA结合的关键位点，证实ZNF180在基因转录调控中发挥直接作用。
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**备注**：以上文献为示例，实际研究中需通过数据库(PubMed、Web of Science)确认具体参考文献。若该蛋白研究较少，可扩展至锌指蛋白家族其他成员的重组表达策略或相关疾病机制研究。
Recombinant human Zinc Finger Protein 180 (ZNF180) is a genetically engineered version of a naturally occurring transcription factor belonging to the C2H2-type zinc finger protein family. Zinc finger proteins are characterized by finger-like structural domains stabilized by zinc ions, which enable sequence-specific DNA binding and regulation of gene expression. ZNF180 contains multiple tandem zinc finger motifs, typically involved in interactions with nucleic acids or proteins, suggesting roles in transcriptional regulation, chromatin remodeling, or RNA processing.
The native ZNF180 gene is expressed in various tissues, including liver, kidney, and heart, and is implicated in cellular processes such as differentiation, proliferation, and apoptosis. Studies associate ZNF180 dysregulation with diseases, particularly cancers, where it may act as a tumor suppressor or oncogene depending on context. Recombinant ZNF180 protein, produced in bacterial or mammalian expression systems, serves as a tool for investigating its molecular mechanisms, DNA-binding specificity, and interactions with signaling pathways.
Research on recombinant ZNF180 aims to elucidate its structural-functional relationships and therapeutic potential. Its conserved zinc finger domains may offer targets for drug development or gene therapy, particularly in malignancies linked to epigenetic or transcriptional abnormalities. Further studies are required to fully unravel its biological significance and clinical relevance.
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