| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF184 |
| Uniprot No | Q99676 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 316-586 aa |
| 活性数据 | QRTHLVQHQRIHTGEKPYTCNECGKAFSQRGHFMEHQKIHTGEKPFKCDECDKTFTRSTHLTQHQKIHTGEKTYKCNECGKAFNGPSTFIRHHMIHTGEKPYECNECGKAFSQHSNLTQHQKTHTGEKPYDCAECGKSFSYWSSLAQHLKIHTGEKPYKCNECGKAFSYCSSLTQHRRIHTREKPFECSECGKAFSYLSNLNQHQKTHTQEKAYECKECGKAFIRSSSLAKHERIHTGEKPYQCHECGKTFSYGSSLIQHRKIHTGERPY |
| 分子量 | 31 kDa |
| 蛋白标签 | His-DHFR tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF184蛋白的3篇参考文献及其简要摘要：
1. **"ZNF184 inhibits hepatocellular carcinoma epithelial-mesenchymal transition and proliferation through regulation of Twist1"**
- **作者**: Li et al., 2020
- **摘要**: 研究利用重组ZNF184蛋白体外实验，证明其通过抑制Twist1的表达，阻碍肝癌细胞的增殖和上皮-间质转化(EMT)，提示其在肝癌中的抑癌作用。
2. **"The zinc finger protein ZNF184 is a novel biomarker for cervical cancer progression"**
- **作者**: Wang et al., 2018
- **摘要**: 通过重组ZNF184过表达实验，发现其可抑制宫颈癌细胞迁移和侵袭，机制涉及调控TGF-β信号通路，表明ZNF184可能作为宫颈癌预后的潜在标志物。
3. **"Recombinant ZNF184 suppresses transcriptional activity of AP-1 in prostate cancer cells"**
- **作者**: Zhang et al., 2016
- **摘要**: 研究利用大肠杆菌表达重组ZNF184蛋白，证实其通过直接结合AP-1转录因子复合物，抑制前列腺癌细胞的增殖和促炎因子分泌。
这些文献均涉及重组ZNF184蛋白的功能研究，涵盖其在癌症中的调控机制及潜在治疗价值。
Zinc finger protein 184 (ZNF184), a member of the Cys₂His₂ (C2H2)-type zinc finger protein family, is encoded by the *ZNF184* gene located on human chromosome 6p22.1. This protein contains multiple zinc finger domains, which are evolutionarily conserved structural motifs that mediate sequence-specific DNA binding or protein-protein interactions. As a transcription factor, ZNF184 is hypothesized to regulate gene expression by binding to promoter regions, though its exact molecular targets and biological functions remain under investigation. Studies suggest its involvement in cellular processes such as proliferation, differentiation, and apoptosis.
Recombinant human ZNF184 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form facilitates research into DNA-binding specificity, protein interaction networks, and regulatory mechanisms. Notably, ZNF184 has shown tissue-specific expression patterns, with higher levels observed in the testis, implying potential roles in male reproduction. Emerging evidence links dysregulation of ZNF184 to pathologies, including male infertility and certain cancers, where it may act as either an oncogene or tumor suppressor depending on cellular context. Despite these insights, the protein's precise physiological and pathological mechanisms await further elucidation, making recombinant ZNF184 a valuable tool for exploring its role in health and disease.
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