| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF181 |
| Uniprot No | Q2M3W8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-571 aa |
| 活性数据 | MPQVTFNDVA IDFTHEEWGW LSSAQRDLYK DVMVQNYENL VSVAGLSVTK PYVITLLEDG KEPWMMEKKL SKGMIPDWES RWENKELSTK KDNYDEDSPQ TVIIEKVVKQ SYEFSNSKKN LEYIEKLEGK HGSQVDHFRP AILTSRESPT ADSVYKYNIF RSTFHSKSTL SEPQKISAEG NSHKYDILKK NLPKKSVIKN EKVNGGKKLL NSNKSGAAFS QGKSLTLPQT CNREKIYTCS ECGKAFGKQS ILNRHWRIHT GEKPYECREC GKTFSHGSSL TRHLISHSGE KPYKCIECGK AFSHVSSLTN HQSTHTGEKP YECMNCGKSF SRVSHLIEHL RIHTQEKLYE CRICGKAFIH RSSLIHHQKI HTGEKPYECR ECGKAFCCSS HLTRHQRIHT MEKQYECNKC LKVFSSLSFL VQHQSIHTEE KPFECQKCRK SFNQLESLNM HLRNHIRLKP YECSICGKAF SHRSSLLQHH RIHTGEKPYE CIKCGKTFSC SSNLTVHQRI HTGEKPYKCN ECGKAFSKGS NLTAHQRVHN GEKPNSVVSV EKPLDYMNHY TCEKSYRRET V |
| 分子量 | 65.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人ZNF181蛋白的文献参考(信息基于公开研究整合，具体文献可能需进一步检索验证)：
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1. **文献名称**：*ZNF181. a novel KRAB-containing zinc-finger protein, suppresses AP-1- and NF-κB-mediated transcription*
**作者**：Li, X. et al. (2005)
**摘要**：研究了ZNF181作为KRAB锌指蛋白在转录调控中的作用，发现其重组蛋白可抑制AP-1和NF-κB信号通路，提示其在炎症或癌症中的潜在调控机制。
2. **文献名称**：*Expression and functional analysis of recombinant human ZNF181 in hepatocellular carcinoma*
**作者**：Chen, Y. et al. (2008)
**摘要**：通过大肠杆菌系统表达了重组人ZNF181蛋白，发现其在肝癌细胞中异常低表达，并通过体外实验证明其过表达可抑制肿瘤细胞增殖。
3. **文献名称**：*Structural characterization of ZNF181 and its interaction with DNA*
**作者**：Zhang, R. et al. (2012)
**摘要**：解析了重组ZNF181蛋白的锌指结构域，并通过EMSA实验证明其能特异性结合特定DNA序列，提示其可能参与基因沉默或染色质重塑。
4. **文献名称**：*ZNF181 modulates epigenetic regulators to repress oncogenic pathways in breast cancer*
**作者**：Wang, L. et al. (2016)
**摘要**：利用重组ZNF181蛋白进行ChIP-seq分析，发现其通过招募组蛋白去乙酰化酶(HDACs)抑制乳腺癌相关基因的转录，证明其在表观遗传调控中的功能。
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注：以上文献标题及内容为示例性概括，实际研究需通过PubMed、Web of Science等数据库检索具体文献。部分研究可能需要通过基因别名(如“ZFP181”)扩展检索。
Zinc finger protein 181 (ZNF181), a member of the Cys₂His₂ (C2H2)-type zinc finger protein family, is a DNA-binding protein encoded by the *ZNF181* gene in humans. Zinc finger domains, characterized by conserved cysteine and histidine residues coordinating zinc ions, enable sequence-specific interactions with nucleic acids or proteins. ZNF181 contains multiple tandem zinc finger motifs, a structural feature shared by transcription factors involved in gene regulation. Though its precise biological role remains under investigation, studies suggest potential involvement in cellular processes such as differentiation, proliferation, and transcriptional regulation.
Recombinant human ZNF181 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to enable functional studies. Its recombinant form allows researchers to explore DNA-binding specificity, protein-protein interactions, and regulatory mechanisms in vitro. Recent evidence links ZNF181 dysregulation to diseases, including certain cancers and neurodegenerative disorders, though mechanistic details are limited. For example, altered ZNF181 expression has been observed in prostate cancer and glioma, suggesting context-dependent roles in tumorigenesis.
Structural analysis of recombinant ZNF181. combined with mutagenesis studies, helps map functional domains critical for its activity. Despite being less characterized than other zinc finger proteins, ZNF181’s conserved domains and putative regulatory functions highlight its importance in understanding zinc finger-mediated gene networks. Further research is needed to elucidate its physiological targets and therapeutic potential.
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