| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF182 |
| Uniprot No | P17025 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639 aa |
| 活性数据 | MTPASASGED SGSFYSWQKA KREQGLVTFE DVAVDFTQEE WQYLNPPQRT LYRDVMLETY SNLVFVGQQV TKPNLILKLE VEECPAEGKI PFWNFPEVCQ VDEQIERQHQ DDQDKCLLMQ VGFSDKKTII TKSARDCHEF GNILHLSTNL VASIQRPDKH ESFGNNMVDN LDLFSRSSAE NKYDNGCAKL FFHTEYEKTN PGMKPYGYKE CGKGLRRKKG LSLHQRIKNG EKPFECTACR KTFSKKSHLI VHWRTHTGEK PFGCTECGKA FSQKSQLIIH LRTHTGERPF ECPECGKAFR EKSTVIIHYR THTGEKPYEC NECGKAFTQK SNLIVHQKTH TGEKTYECTK CGESFIQKLD LIIHHSTHTG KKPHECNECK KTFSDKSTLI IHQRTHTGEK PHKCTECGKS FNEKSTLIVH QRTHTGEKPY ECDVCGKTFT QKSNLGVHQR THSGEKPFEC NECEKAFSQK SYLMLHQRGH TGEKPYECNE CEKAFSQKSY LIIHQRTHTE EKPYKCNECG KAFREKSKLI IHQRIHTGEK PYECPVCWKA FSQKSQLIIH QRTHTGEKPY ACTECGKAFR EKSTFTVHQR THTGEKPYKC TECGKAFTQK SNLIVHQRTH AGKKAHGRGH TRKSKFMAH |
| 分子量 | 73.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF182蛋白的3篇假设性参考文献示例(请注意，以下内容为模拟生成，实际文献需通过数据库验证)：
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1. **文献名称**: *Cloning and functional characterization of recombinant human ZNF182 protein*
**作者**: Lee, S., et al.
**摘要**: 研究报道了ZNF182基因的克隆及其在大肠杆菌中的重组表达与纯化。通过电泳迁移率实验(EMSA)证明其具有特异性DNA结合活性，并发现该蛋白在调控细胞增殖相关基因中可能发挥作用。
2. **文献名称**: *ZNF182 as a potential tumor suppressor in hepatocellular carcinoma*
**作者**: Chen, X., et al.
**摘要**: 研究表明ZNF182在肝癌组织中表达显著下调。通过体外重组ZNF182蛋白实验发现，其过表达可诱导肿瘤细胞凋亡并抑制迁移，提示其可能通过调控Wnt/β-catenin通路发挥抑癌作用。
3. **文献名称**: *Structural analysis of human ZNF182 zinc finger domains*
**作者**: Wang, Y., et al.
**摘要**: 利用X射线晶体学解析了ZNF182蛋白的锌指结构域，揭示了其与DNA结合的分子机制，为设计针对ZNF182功能异常相关疾病的干预策略提供了结构基础。
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建议通过PubMed、Web of Science等平台检索实际文献时结合关键词"ZNF182"、"recombinant protein"及"zinc finger"。若该蛋白研究较少，可扩展至锌指蛋白家族功能或相关疾病通路的文献。
Zinc finger protein 182 (ZNF182), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, is encoded by the ZNF182 gene located on human chromosome 9q34.3. This protein belongs to the C2H2-type zinc finger transcription factor superfamily, characterized by tandem arrays of zinc finger motifs that mediate sequence-specific DNA binding. The KRAB domain at its N-terminus is known to recruit transcriptional corepressors, suggesting a role in gene silencing or chromatin remodeling. While the biological functions of ZNF182 remain less characterized compared to other zinc finger proteins, studies suggest its potential involvement in transcriptional regulation, cellular differentiation, and tumorigenesis. Notably, abnormal expression patterns of ZNF182 have been observed in several cancers, including prostate and gastric cancers, implicating it as a possible biomarker or regulatory factor in oncogenesis. Recombinant ZNF182 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies, enabling researchers to investigate its DNA-binding specificity, protein interaction networks, and regulatory mechanisms. Current research focuses on elucidating its target genes, post-translational modifications, and potential roles in cellular stress responses or epigenetic regulation, providing insights into its pathophysiological relevance.
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