| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF254 |
| Uniprot No | O75437 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-659 aa |
| 活性数据 | MPGPPRSLEM GLLTFRDVAI EFSLEEWQHL DIAQQNLYRN VMLENYRNLA FLGIAVSKPD LITCLEQGKE PWNMKRHEMV DEPPGMCPHF AQDLWPEQGM EDSFQKAILR RYGKYGHENL QLRKGCKSVD EYKVNKEGYN GLNQCFTTAQ SKVFQCDKYL KVFYKFLNSN RPKIRHTEKK SFKCKKRVKL FCMLSHKTQH KSIYHREKSY KCKECGKTFN WSSTLTNHRK IYTEEKPYKC EEYNKSPKQL STLTTHEIIH AGEKLYKCEE CGEAFNRSSN LTTHKIIHTG EKPYKCEECG KAFIWSSTLT EHKKIHTRKK PYKCEECGKA FIWSSTLTRH KRMHTGEKPY KCEECGKAFS QSSTLTTHKI IHTGEKRYKC LECGKAFKQL STLTTHKIIH VGEKLYKCEE CGKGFNRSSN LTTHKIIHTG EKPYKCEECG KAFIWSSTLT KHKRIHTREK PYKCEECGKA FIWSSTLTRH KRMHTGEKPY KCEECGKSFS QSSTLTTHKI IHTGEKPYKC EECGKAFNWS STLTKHKIIH TEEKPYKCEK CGKAFKQSSI LTNHKRIHTG EKPYKCEECG KSFNRSSTFT KHKVIHTGVK PYKCEECGKA FFWSSTLTKH KRIHTGEQPY KWEKFGKAFN RSSHLTTDKI THWREILQV |
| 分子量 | 77.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF254蛋白的3条模拟参考文献，基于领域内相关研究的典型方向(注：文献为模拟示例，非真实存在)：
1. **文献名称**：Structural and Functional Characterization of ZNF254 as a Transcriptional Repressor
**作者**：Chen L, et al.
**摘要**：该研究利用重组ZNF254蛋白解析其KRAB结构域与KAP1蛋白的相互作用机制，证实ZNF254通过招募KAP1介导组蛋白H3K9三甲基化，调控靶基因沉默，揭示了其在表观遗传抑制中的功能。
2. **文献名称**：Genome-wide Binding Analysis of ZNF254 Reveals Roles in Heterochromatin Formation
**作者**：Wang Y, et al.
**摘要**：通过重组ZNF254蛋白的ChIP-seq实验，发现其优先结合基因启动子及重复元件区域，表明其参与异染色质形成和转座子沉默，为理解ZNF254在基因组稳定性中的作用提供证据。
3. **文献名称**：Expression and Purification of Recombinant ZNF254 in Mammalian Cells for Functional Studies
**作者**：Smith J, et al.
**摘要**：开发了一种基于HEK293细胞的重组ZNF254蛋白高效表达纯化系统，验证其核定位特性及DNA结合活性，为后续生化研究提供高质量蛋白制备方案。
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**备注**：以上为模拟生成内容，实际研究需查阅真实数据库(如PubMed)。ZNF254研究相对较少，建议拓展检索其所属的KRAB-ZFP家族文献或通过UniProt (ID: Q9UBG7) 关联引用论文。
ZNF254. also known as ORF19 or ZKSCAN8. is a human protein belonging to the zinc finger protein family, characterized by conserved C2H2-type zinc finger domains that facilitate nucleic acid binding. This protein is encoded by the ZNF254 gene located on chromosome 19q13.4. a region linked to several neurological disorders and cancers. Functionally, ZNF254 is hypothesized to act as a transcription factor, regulating gene expression through interactions with DNA or RNA. Studies suggest its involvement in cellular processes like differentiation, apoptosis, and chromatin remodeling, though its precise molecular mechanisms remain under investigation.
Interest in ZNF254 has grown due to its potential dual role in cancer. Some research indicates it may act as a tumor suppressor, with downregulation observed in colorectal cancer and gliomas, often associated with promoter hypermethylation. Conversely, other studies report its upregulation in specific malignancies, implying context-dependent oncogenic properties. Recombinant ZNF254 protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, followed by affinity chromatography purification. This engineered protein enables structural analysis, interaction studies, and functional assays to elucidate its roles in disease pathways. Current research focuses on clarifying its regulatory targets and therapeutic potential, particularly in cancers and neurodevelopmental disorders linked to 19q13.4 alterations.
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