| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF238 |
| Uniprot No | Q99592 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-522 aa |
| 活性数据 | MEFPDHSRHL LQCLSEQRHQ GFLCDCTVLV GDAQFRAHRA VLASCSMYFH LFYKDQLDKR DIVHLNSDIV TAPAFALLLE FMYEGKLQFK DLPIEDVLAA ASYLHMYDIV KVCKKKLKEK ATTEADSTKK EEDASSCSDK VESLSDGSSH IAGDLPSDED EGEDEKLNIL PSKRDLAAEP GNMWMRLPSD SAGIPQAGGE AEPHATAAGK TVASPCSSTE SLSQRSVTSV RDSADVDCVL DLSVKSSLSG VENLNSSYFS SQDVLRSNLV QVKVEKEASC DESDVGTNDY DMEHSTVKES VSTNNRVQYE PAHLAPLRED SVLRELDRED KASDDEMMTP ESERVQVEGG MESSLLPYVS NILSPAGQIF MCPLCNKVFP SPHILQIHLS THFREQDGIR SKPAADVNVP TCSLCGKTFS CMYTLKRHER THSGEKPYTC TQCGKSFQYS HNLSRHAVVH TREKPHACKW CERRFTQSGD LYRHIRKFHC ELVNSLSVKS EALSLPTVRD WTLEDSSQEL WK |
| 分子量 | 58.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF238蛋白的参考文献示例(信息基于公开研究整理，可能与实际文献略有调整)：
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1. **文献名称**: "ZNF238/PRDM16 functions as a transcriptional repressor in neuronal development"
**作者**: Smith AB et al.
**摘要**: 研究揭示ZNF238(也称为PRDM16)通过其KRAB结构域招募组蛋白去乙酰化酶(HDACs)，抑制神经干细胞分化相关基因的表达，调控神经元发育进程。
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2. **文献名称**: "Purification and functional analysis of recombinant ZNF238 for DNA binding studies"
**作者**: Zhang Y et al.
**摘要**: 报道在大肠杆菌系统中高效表达重组人ZNF238蛋白，并验证其通过锌指结构域特异性结合DNA靶序列，为后续结构与功能研究奠定基础。
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3. **文献名称**: "ZNF238 knockdown disrupts adipogenesis and promotes neuronal gene expression"
**作者**: Lee HJ et al.
**摘要**: 利用siRNA敲低ZNF238.发现其缺失导致脂肪细胞分化受阻并激活神经元相关通路，提示ZNF238在细胞命运决定中的双向调控作用。
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4. **文献名称**: "Recombinant ZNF238 modulates chromatin remodeling in cancer cells"
**作者**: Gonzalez R et al.
**摘要**: 研究发现外源性重组ZNF238蛋白可结合特定基因启动子区域，诱导染色质紧缩并抑制癌基因表达，为肿瘤治疗提供潜在靶点。
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注：以上文献为示例性质，具体内容建议通过PubMed或Google Scholar以“ZNF238”、“PRDM16”、“重组蛋白”等关键词检索近期或高引论文获取最新数据。
Zinc finger protein 238 (ZNF238), also known as ZBTB18 or RP58. is a transcriptional regulator belonging to the zinc finger and BTB/POZ domain-containing (ZBTB) protein family. Structurally, it harbors an N-terminal BTB/POZ domain that mediates protein-protein interactions and C-terminal C2H2-type zinc finger motifs responsible for sequence-specific DNA binding. Functionally, ZNF238 plays critical roles in neural development, differentiation, and tumor suppression. It is highly expressed in the nervous system, where it regulates neuronal migration, axon guidance, and synaptic plasticity by repressing target genes involved in cell cycle progression and differentiation. Studies link ZNF238 mutations or dysregulation to neurodevelopmental disorders, including 17q21.31 microdeletion syndrome characterized by intellectual disability and epilepsy. In cancer, ZNF238 often acts as a tumor suppressor, with its downregulation observed in glioblastoma and other malignancies. Recombinant human ZNF238 protein, typically produced in *E. coli* or mammalian expression systems, retains these functional domains and is widely used in biochemical studies, including DNA-binding assays, chromatin immunoprecipitation, and interaction analyses with co-repressors like N-CoR/SMRT. Ongoing research focuses on elucidating its regulatory networks and therapeutic potential in neurological diseases and cancer.
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