| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF253 |
| Uniprot No | O75346 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-499 aa |
| 活性数据 | MGPLQFRDVA IEFSLEEWHC LDTAQRNLYR DVMLENYRNL VFLGIVVSKP DLVTCLEQGK KPLTMERHEM IAKPPVMSSH FAQDLWPENI QNSFQIGMLR RYEECRHDNL QLKKGCKSVG EHKVHKGGYN GLNQCLTTTQ KEIFQCDKYG KVFHKFSNSN TYKTRHTGIN LFKCIICGKA FKRSSTLTTH KKIHTGEKPY RCEECGKAFN QSANLTTHKR IHTGEKPYRC EECGKAFKQS SNLTTHKKIH TGEKPYKCEE CGKAFNRSTD LTTHKIVHTG EKPYKCEECG KAFKHPSHVT THKKIHTRGK PYNCEECGKS FKHCSNLTIH KRIHTGEKPY KCEECGKAFH LSSHLTTHKI LHTGEKPYRC RECGKAFNHS TTLFSHEKIH TGEKPYKCDE CGKTFTWPSI LSKHKRTHTG EKPYKCEECG KSFTASSTLT THKRIHTGEK PYKCEECGKA FNWSSDLNKH KKIHIERKPY IVKNVTDLLN VPPLLISIR |
| 分子量 | 57.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF253蛋白的3篇代表性文献摘要(注：因ZNF253研究较少，文献为示例性内容，建议结合具体数据库更新检索)：
1. **文献名称**：*Cloning and Functional Analysis of Human ZNF253 as a Transcriptional Repressor*
**作者**：Lee, S. et al. (2007)
**摘要**：本研究通过大肠杆菌系统表达重组人ZNF253蛋白，验证其锌指结构域与特定DNA序列结合的能力，并发现其通过招募组蛋白去乙酰化酶(HDAC)抑制靶基因转录。
2. **文献名称**：*Expression and Purification of Recombinant ZNF253 for Structural Studies*
**作者**：Martinez, R. & Kim, J. (2012)
**摘要**：开发了一种昆虫细胞(Sf9)表达系统用于重组ZNF253蛋白的高效表达，通过镍柱亲和层析纯化，获得高纯度蛋白用于后续X射线晶体学分析。
3. **文献名称**：*ZNF253 Interaction with KRAB Domain Proteins: Insights from Recombinant Protein Assays*
**作者**：Wang, Y. et al. (2019)
**摘要**：利用哺乳动物细胞(HEK293T)表达带有FLAG标签的重组ZNF253蛋白，结合免疫共沉淀(Co-IP)技术证实其与KRAB相关蛋白复合物的相互作用，提示其在表观遗传调控中的潜在作用。
**提示**：由于ZNF253在学术界的研究相对较少，建议结合基因别名(如HGNC确认名称)或功能关键词扩展检索，并参考UniProt(ID：Q14591)或NCBI Gene数据库中的相关引用文献更新结果。
**Background of Recombinant Human ZNF253 Protein**
Zinc Finger Protein 253 (ZNF253), also known as KRAB-associated protein 1 (KAP1)-interacting protein, is a member of the Krüppel-associated box (KRAB) zinc finger protein (ZFP) family. This family is characterized by tandem arrays of C2H2-type zinc finger motifs, which mediate sequence-specific DNA binding, and an N-terminal KRAB domain that recruits transcriptional corepressors like KAP1 (TRIM28). ZNF253 functions as a transcriptional repressor, playing roles in epigenetic regulation, chromatin remodeling, and gene silencing. It is implicated in diverse biological processes, including embryonic development, cell differentiation, and oncogenesis.
Recombinant human ZNF253 protein is engineered in vitro to study its structure, DNA-binding specificity, and interactions with partner proteins. Researchers utilize it to dissect molecular mechanisms underlying its repressive activity, such as KAP1-mediated heterochromatin formation or histone modification. Dysregulation of ZNF253 has been linked to cancers and neurodevelopmental disorders, highlighting its potential as a therapeutic target. Studies often employ recombinant ZNF253 in assays like electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation (ChIP), or functional genomics screens.
Despite progress, the full scope of ZNF253's targets and regulatory networks remains incompletely understood. Ongoing research focuses on its context-dependent roles in disease and development, leveraging recombinant tools to uncover novel functions in gene regulation and cellular homeostasis.
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