| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF257 |
| Uniprot No | Q9Y2Q1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-563 aa |
| 活性数据 | MGPLTIRDVT VEFSLEEWHC LDTAQQNLYR DVMLENYRNL VFLGIAVSKP DLITCLEQGK EPCNMKRHEM VAKPPVMCSH IAEDLCPERD IKYFFQKVIL RRYDKCEHEN LQLRKGCKSV DECKVCKGGY NGLNQCLITT QSKMYQCDKY VKVFYKFSNS DRHKIRHTEK KTCKCKECGK SFCMLSQLTR HKRIHIRENS HKCEECGKAF NQSSALTRHK MTHTGEKPYK CEECGKAFNR SSHLTQHKVI HTREKPYKCE ECGKAFNRSS HITQHKRIHN REKPFKYDEC CKAFKWSSAL TTLTQHKRIH TGEKPYKCEE CGKAFNQSSA LTRHKMIHTG EKPFQCEECG KAFNRSSHLT QHKIIHTKEK PYKCEECGKA FNRSSHLTKH KRIHTREKAY KCDEYCKAFN WSSALTTLTQ HKIIHTGEKP YKCEECGKAF NRSSYLIRHK IIHTGEKPYK CEECGKAFNQ SSHLTQHKII HTGEKPYKCE ECGKAFNRSS HLSQHKIIHT GEKPYKCEEC GKPFNRFSYL TVHKRIHAGE NPNKYEECGK ACNHSSNLTK HNS |
| 分子量 | 65.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的参考文献示例(基于锌指蛋白研究领域的常见模式，ZNF257相关文献可能有限)：
1. **"Expression and Functional Characterization of Recombinant Human ZNF257 Protein in Eukaryotic Cells"**
*作者：Chen X, et al.*
摘要：研究利用哺乳动物表达系统成功制备重组人ZNF257蛋白，证实其通过锌指结构域结合特定DNA序列，调控下游靶基因的转录活性，可能参与细胞分化过程。
2. **"Purification and Structural Analysis of ZNF257 Recombinant Protein Using CRISPR/Cas9-Modified Cell Lines"**
*作者：Park H, et al.*
摘要：通过CRISPR技术构建稳定表达重组ZNF257的HEK293细胞系，优化纯化流程并利用质谱分析其翻译后修饰，揭示其磷酸化对DNA结合能力的影响。
3. **"ZNF257 Recombinant Protein Suppresses Tumor Growth via Epigenetic Regulation in Mouse Models"**
*作者：Li Y, et al.*
摘要：在大肠杆菌中表达带有GST标签的重组ZNF257蛋白，体内实验表明其通过招募组蛋白去乙酰化酶(HDACs)抑制肿瘤相关基因表达，具有抗癌潜力。
4. **"Development of a High-Throughput Assay for ZNF257-DNA Interaction Using Recombinant Protein"**
*作者：Müller T, et al.*
摘要：建立基于表面等离子共振(SPR)的检测方法，分析重组ZNF257蛋白与不同DNA元件的结合动力学，为锌指蛋白的工程化应用提供数据支持。
**注**：以上为模拟示例，实际文献可能有限。建议通过 **PubMed** 或 **Google Scholar** 使用关键词 "ZNF257 recombinant" / "ZNF257 protein function" 获取最新研究。若具体数据不足，可扩展至锌指蛋白家族(如ZNF256/ZNF263等)的研究作为参考。
Zinc finger protein 257 (ZNF257) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZFP) family, one of the largest transcription factor families in humans. Structurally, it contains multiple C2H2-type zinc finger motifs, which enable sequence-specific DNA binding, and a KRAB domain at the N-terminus that typically mediates transcriptional repression by recruiting chromatin-modifying complexes. ZNF257 is encoded by a gene located on human chromosome 19q13.43. a region associated with several disease-linked genetic variations. While its precise biological functions remain incompletely characterized, ZNF257 is hypothesized to regulate gene expression during development, cellular differentiation, or stress responses, as suggested by the conserved domain architecture shared across KRAB-ZFPs.
Recombinant ZNF257 protein, produced via expression systems like E. coli or mammalian cells, serves as a critical tool for studying its DNA-binding specificity, interaction partners, and regulatory mechanisms. Functional studies using recombinant variants (e.g., domain-deletion mutants) have begun to dissect structure-function relationships. However, challenges persist in mapping its endogenous target genes and understanding its role in pathological contexts, such as cancer or neurological disorders, where ZFPs often exhibit dysregulation. Current research aims to integrate proteomic, genomic, and CRISPR-based screening approaches to unravel ZNF257's physiological and pathological significance.
×
